Nitrogen Removal in Intermittently Aerated Biofilm Airlift Reactor
Publication: Journal of Environmental Engineering
Volume 124, Issue 3
Abstract
Denitrification was integrated in a nitrifying biofilm airlift suspension reactor by intermittent aeration. A total nitrogen-removal efficiency of 75% was reached at an aerobic ammonia load of 5 kgN ⋅ m−3 ⋅ d−1. Acetate was added to the reactor during the anoxic periods. The system was operated at sufficiently long anoxic hydraulic retention times to allow suspended growth of denitrifying heterotrophs. However, the heterotrophic biomass did not grow in suspension but grew as protuberances and layers on the nitrifying biofilms. Nitrification and denitrification occurred via nitrite as an intermediate, possibly because denitrifiers outcompeted the nitrite oxidizers. Because of the establishment of the “nitrite route,” a low average COD/N ratio of 2.6 kgCOD ⋅ was obtained. When denitrifying operation with acetate addition was started, initially almost all nitrogen removed was emitted as nitrous oxide. Subsequently, the nitrous oxide emission strongly decreased to almost zero in a period of 30 days, while the nitrogen-removal efficiency was maintained.
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References
1.
Abeling, U., and Seyfried, C. F. (1992). “Anaerobic-aerobic treatment of high-strength ammonium wastewater—nitrogen removal via nitrite.”Water Sci. and Technol., 26(5-6), 1007–1015.
2.
Alleman, J. E.(1984). “Elevated nitrite occurrence in biological wastewater treatment systems.”Water Sci. and Technol., 17(2/3), 409–419.
3.
Anthonisen, A. C., Loehr, R. C., Prakasam, T. B. S., and Srinath, E. G.(1976). “Inhibition of nitrification by ammonia and nitrous acid.”J. Water Pollution Control Fed., 48(5), 835–852.
4.
Bennemann, H. (1990). “Untersuchungen zur Reaktions- und Verfahrenstechnik von Nitrifikation und Denitrifikation im Drei-Phasen-Wirbelbettreaktor,” PhD dissertation, University of Paderborn, Germany.
5.
Chen, S.-K., Juaw, C.-K., and Cheng, S.-S.(1991). “Nitrification and denitrification of high-strength ammonium and nitrite wastewater with biofilm reactors.”Water Sci. Technol., 23(7/9), 1417–1425.
6.
Czepiel, P., Crill, P., and Harriss, R.(1995). “Nitrous oxide emissions from municipal wastewater treatment.”Envir. Sci. Technol., 29(9), 2352–2356.
7.
Furumai, H., and Rittmann, B. E. (1992). “Advanced modelling of mixed populations of heterotrophs and nitrifiers considering the formation and exchange of soluble microbial products.”Water Sci. and Technol., 26(3-4), 493–502.
8.
Garrido, J. M., Campos, J. L., Méndez, R., and Lema, J. M.(1997a). “Nitrous oxide production by nitrifying biofilms in a biofilm airlift suspension reactor.”Water Sci. and Technol., 36(1), 157–163.
9.
Garrido, J. M., van Benthum, W. A. J., van Loosdrecht, M. C. M., and Heijnen, J. J.(1997b). “Influence of dissolved oxygen concentration on nitrite accumulation in a biofilm airlift suspension reactor.”Biotechnol. Bioengrg., 53(3), 168–178.
10.
Graedel, T. E., and Crutzen, P. J. (1995). Atmosphere, climate, and change. Scientific American Library, New York, N.Y.
11.
Hao, O. J., and Huang, J.(1996). “Alternative aerobic-anoxic process for nitrogen removal: Process evaluation.”Water Envir. Res., 68(1), 83–93.
12.
Heijnen, J. J. (1996). “Scale-up aspects of immobilized cell reactors.”Immobilized cells: basics and applications. R. H. Wijffels, R. M. Buitelaar, C. Bucke, and J. Tramper, eds., Progress in Biotechnology 11, Elsevier Science Publishers, Amsterdam, The Netherlands, 497–504.
13.
Heijnen, S. J., Mulder, A., Weltevrede, R., Hols, P. H., and van Leeuwen, H. L. J. M.(1990). “Large-scale anaerobic/aerobic treatment of complex industrial wastewater using immobilized biomass in fluidized bed and air-life suspension reactors.”Chem. Engrg. Technol., 13(3), 202–208.
14.
Hochstein, L. I., Betlach, M., and Kritikos, G.(1984). “The effect of oxygen on denitrification during steady-state growth of Paracoccus halodenitrificans.”Archives of Microbiology, 137(1), 74–78.
15.
Hooper, A. B., Vannelli, T., Bergmann, D. J., and Arciero, D. M. (1997). “Enzymology of the oxidation of ammonia to nitrite by bacteria.”Antonie van Leeuwenhoek, 71(1-2), 59–67.
16.
Jetten, M. S. M., Logemann, S., Muyzer, G., Robertson, L. A., de Vries, S., van Loosdrecht, M. C. M., and Kuenen, J. G. (1997). “Novel principles in the microbial conversion of nitrogen compounds.”Antonie van Leeuwenhoek, 71(1-2), 75–93.
17.
Otte, S., Grobben, N. G., Robertson, L. A., Jetten, M. S. M., and Kuenen, J. G.(1996). “Nitrous oxide production by Alcaligenes faecalis under transient and dynamic aerobic and anaerobic conditions.”Appl. Envir. Microbiology, 62(7), 2421–2426.
18.
Tijhuis, L., Huisman, J. L., Hekkelman, H. D., van Loosdrecht, M. C. M., and Heijnen, J. J.(1995). “Formation of nitrifying biofilms on small suspended particles in airlift reactors.”Biotechnol. Bioengrg., 47(5), 585–595.
19.
Tijhuis, L., van Loosdrecht, M. C. M., and Heijnen, J. J.(1994). “Formation and growth of heterotrophic aerobic biofilms on small suspended particles in airlift reactors.”Biotechnol. Bioengrg., 44(5), 595–608.
20.
van Benthum, W. A. J., Derissen, B. P., van Loosdrecht, M. C. M., and Heijnen, J. J. (1998). “Nitrogen removal using nitrifying biofilm growth and denitrifying suspended growth in a biofilm airlift suspension reactor coupled with a chemostat.”Water Res.
21.
van Benthum, W. A. J., Garrido-Fernández, J. M., Tijhuis, L., van Loosdrecht, M. C. M., and Heijnen, J. J.(1996). “Formation and detachment of biofilms and granules in a nitrifying biofilm airlift suspension reactor.”Biotechnol. Prog., 12(6), 764–772.
22.
van Benthum, W. A. J., van Loosdrecht, M. C. M., and Heijnen, J. J.(1997a). “Control of heterotrophic layer formation on nitrifying biofilms in a biofilm airlift suspension reactor.”Biotechnol. Bioengrg., 53(4), 397–405.
23.
van Benthum, W. A. J., van Loosdrecht, M. C. M., and Heijnen, J. J.(1997b). “Process design for nitrogen removal using nitrifying biofilm and denitrifying suspended growth in a biofilm airlift suspension reactor.”Water Sci. and Technol., 36(1), 119–128.
24.
van Loosdrecht, M. C. M., and Heijnen, J. J.(1993). “Biofilm bioreactors for wastewater treatment.”TIBTECH, 11(4), 117–121.
25.
van Loosdrecht, M. C. M., Pot, M. A., and Heijnen, J. J.(1997). “Importance of bacterial storage polymers in bioprocesses.”Water Sci. and Technol., 35(1), 41–47.
26.
v. Münch, E., Lant, P., and Keller, J.(1996). “Simultaneous nitrification and denitrification in bench-scale sequencing batch reactors.”Water Res., 30(2), 277–284.
27.
von Schulthess, R., Wild, D., and Gujer, W.(1994). “Nitric and nitrous oxides from denitrifying activated sludge at low oxygen concentration.”Water Sci. and Technol., 30(6), 123–132.
28.
Wanner, O., and Reichert, P.(1996). “Mathematical modelling of mixed-culture biofilms.”Biotechnol. Bioengrg., 49(2), 172–184.
29.
Wiesmann, U. (1994). “Biological nitrogen removal from wastewater.”Advances in biochemical engineering biotechnology, A. Fiechter, ed., Vol. 51, Springer-Verlag, Berlin, Germany, 113–154.
30.
Zhang, T. C., Fu, Y.-C., and Bishop, P. L.(1995). “Competition for substrate and space in biofilms.”Water Envir. Res., 67(6), 992–1003.
31.
Zheng, H., Hanaki, K., and Matsuo, T.(1994). “Production of nitrous oxide gas during nitrification of wastewater.”Water Sci. and Technol., 30(6), 133–141.
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Copyright © 1998 American Society of Civil Engineers.
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Published online: Mar 1, 1998
Published in print: Mar 1998
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