TECHNICAL PAPERS
Sep 1, 1992

Bioleaching of Metals from Sewage Sludge by Sulfur‐Oxidizing Bacteria

Publication: Journal of Environmental Engineering
Volume 118, Issue 5

Abstract

Twenty‐three different sewage sludges were studied to adapt indigenous sulfur‐oxidizing microflora to solubilize a substantial part of trace metals from the sludges. The results showed that the oxidation of elemental sulfur into sulfuric acid with indigenous sludge thiobacilli causes a sludge acidification, lowering the pH below 2.0. This pH reduction solubilizes trace metals to a level compatible with recommended norms for agricultural use with the following metal solubilization efficiencies: Cd, 82±13%; Cr, 31±19%; Cu, 75±21%; Mn, 92±7%; Ni, 70±16%; Pb, 27±21%; and Zn, 85±10%. The adaptation of this microflora reduces the trace‐metal‐leaching period to 1.5 to five days, depending on the sludge used. The growth and metal‐leaching capacity of the adapted micro‐flora, which is a mixed culture of thiobacilli, was not significantly influenced by the sludge type (nondigested sludge, aerobically or anaerobically digested sludge) and by sludge total solids content.

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References

1.
Adamu, C. A., Bell, P. F., and Mulchi, C. (1989). “Residual metal concentrations in soils and leaf accumulations in Tobacco a decade following farmland application of municipal sludge.” Envir. Pollut., 56, 113–126.
2.
Ahonen, L., and Tuovinen, O. H. (1990). “Kinetics of sulfur oxidation at suboptimal temperatures.” Appl. Envir. Microbiology, 56(3), 560–562.
3.
Blais, J. F., Auclair, J. C., and Tyagi, R. D. (1992a). “Cooperation between two Thiobacillus strains for trace metals removal from municipal sludge.” Can. J. Microbiology., 38(3), 181–187.
4.
Blais, J. F., Tyagi, R. D., and Auclair, J. C. (1992b). “Indicator bacteria reduction in sewage sludge by a metal bioleaching process.” Water Res., 26(4), 487–495.
5.
Bosecker, K. (1986). “Bacterial metal recovery and detoxification of industrial waste.” Biotechn. Bioengrg. Symp., 16, 105–120.
6.
Bruce, A. M., and Davis, R. D. (1989). “Sewage sludge disposal: Current and future options.” Water Sci. Tech., 21(10/11), 1113–1128.
7.
Brune, D. C. (1989). “Sulfur oxidation by phototrophic bacteria.” Biochem. Biophys. Acta. 975, 189–221.
8.
Bryant, R. D., Costerton, J. W., and Laishley, E. J. (1984). “The role of Thiobacillus albertis glycocalyx in the adhesion of cells to elemental sulfur.” Can. J. Microbiology, 30(1), 81–90.
9.
Bryant, R. D., McGroarty, K. M., Costerton, J. W., and Laishley, E. J. (1983). “Isolation and characterization of a new acidophilic Thiobacillus species (T. albertis).” Can. J. Microbiology, 29(9), 1159–1170.
10.
Colmer, A. R., and Hinkle, M. E. (1947). “The role of microorganism in acid mine drainage.” Science, 106, 253–256.
11.
Couillard, D., and Mercier, G. (1990). “Bacterial leaching of heavy metals from sewage sludge‐bioreactors comparison.” Envir. Pollut., 66, 237–253.
12.
Couillard, D., and Mercier, G. (1991a). “Optimum residence time (in CSTR and airlift reactor) for bacterial leaching of metals from sewage sludge.” Water Res., 25(2), 211–218.
13.
Couillard, D., and Mercier, G. (1991b). “Procédé de solubilisation bioligique des métaux dans les bous anéerobies d'épuration: Filtrabilité, neutralisation et teneurs en N et P des boues traitées.” Can. J. Chem. Engrg, 69(6), 779–787 (in French).
14.
Davis, R. D. (1987). “Use of sewage sludge on land in the United Kingdom.” Water Sci. Technl., 19, 1–8.
15.
Flynn, F., Jalbert, J. M., Robert, J., St‐Yves, A., Terreault, A., and Trudel, G. (1987). Valorisation agricole des boues de stations d'épuration des eaux usées municipales, Guide de bonnes pratiques. Ministère de l'Environnement du Québec, Quebec City, Quebec (in French).
16.
“The 40 city study.” (1982). Fate of priority pollutants in publicy owned treatment works (EPA 440/1‐82‐303), Vol. 1, Industrial Tech. Div., Envir. Protection Agency, Washington, D.C.
17.
Hutchins, S. R., Davidson, M. S., Brierley, J. A., and Brierley, C. L. (1986). “Microorganisms in reclamation of metals.” Annual Rev. Microbiology, 40, 311–336.
18.
Hutchinson, M., Johnstone, K. I., and White, D. (1969). “Taxonomy of the genus Thiobacilhis: The outcome of numerical taxonomy applied to the group as a whole.” J. Gen. Microbiology, 57(3), 397–410.
19.
Janzen, H. H., and Bettany, J. R. (1987). “Oxidation of elemental sulfur under field conditions in central Saskatchewan.” Can. J. Soil Sci., 67(3), 609–618.
20.
Jenkins, R. L., Benjamin, J. S., Marvin, L. S., Rodger, B., Lo, M. P., and Huang, R. T. (1981). “Metal removal and recovery from municipal sludge.” J. Water Pollut. Control Fed., 53(1), 25–32.
21.
Kelly, D. P., and Harrison, A. P. (1988). “Genus Thiobacilhis.” Bergey's manual of determinative bacteriology,]. G. Holt, J. T. Staley, M. P. Bryant, and N. Pfennig eds., Williams & Wilkins Co., Baltimore, Md., Vol. 3, 1842–1858.
22.
Khalid, Z. M., and Malik, K. A. (1987). “Isolation and characterization of some acidophilic thiobacilli from sewage waters.” Pakistan J. Sci. Ind. Res., 30(12), 905–908.
23.
Kreye, W. C., King, P. H., and Randall, C. W. (1973). “Biological treatment of high thiosulfate industrial waste water.” Proc. 28th Ind. Waste Cottf., Purdue University, 537–547.
24.
Levine, M. B., Hall, A. T., Barrett, G. W., and Taylor, D. H. (1989). “Heavy metal concentrations during ten years of sludge treatment to an old‐field community.” J. Envir. Qual., 18(4), 411–418.
25.
Matin, A. (1978). “Organic nutrition of chemolithotrophic bacteria.” Annu. Rev. Microbiology, 32, 433–468.
26.
Mininni, G., and Santori, M. (1987). “Problems and perspectives of sludge utilization in agriculture.” Ecosystem Envir., 18, 291–311.
27.
“National sewage sludge survey; availability of information and data, and anticipated impacts on proposed regulations, proposed rule.” (1990). U.S. Federal Register, 55(218), 47209–47283.
28.
Sand, W. (1987). “Importance of hydrogen sulfide, thiosulfate, and methylmercaptan for growth of thiobacilli during simulation of concrete corrosion.” Appl. Envir. Microbiology, 53(7), 1645–1648.
29.
Scheltinga, H. M. J. (1987). “Sludge in agriculture: The European approach.” Water Sci. Techn., 19(8), 9–18.
30.
Schönborn, W., and Hartmann, H. (1978). “Bacterial leaching of metals from sewage sludge.” Eur. J. Apl. Microbiology and Biotech., 5, 305–313.
31.
“Sludge treatment and disposal—Process design manual.” (1979). EPA‐625/1‐79‐011, Ctr. for Envir. Res. Information, Envir. Protection Agency, Cincinnati, Ohio.
32.
Standards methods for examination of water and wastewaters. (1989). 17th Ed., Am. Public Health Assoc./Am. Water Works Assoc./Water Pollut. Control Fed., Washington, D.C.
33.
Sublette, K. L., and Sylvester, N. D. (1987). “Oxidation of hydrogen sulfide by Thiobacilhis denitrifleans: Desulfurization of natural gas.” Biotech. Bioengrg., 29, 249–257.
34.
Takakuwa, S., Fujimori, T., and Iwasaki, H. (1979). “Some properties of cell‐sulfur adheson in Thiobacilhis thiooxidans.” J. Gen. Appl. Microbiology, 25, 21–29.
35.
Tyagi, R. D., and Couillard, D. (1989). “Bacterial leaching of metals from sludge.” Wastewater treatment technology, Encyclopedia of environmental control technology, Vol. 3, P. E. Cheremisinoff, ed., Gulf Publishing Co., Houston, Tex., pp. 557–591.
36.
Tyagi, R. D., and Tran, F. T. (1991). “Microbial leading of metals from digested sewage sludge in continuous system.” Envir. Techn., 12(4), 303–312.
37.
Tyagi, R. D., Couillard, D., and Grenier, Y. (1991a). “Effects of medium composition on the bacterial leaching of metals from the digested sludge.” Envir. Pollut., 71, 57–67.
38.
Tyagi, R. D., Couillard, D., and Tran, F. T. (1988). “Heavy metals removal from anaerobically digested sludge by chemical and microbiological methods.” Envir. Pollut., 50, 295–316.
39.
Tyagi, R. D., Couillard, D., and Tran, F. T. (1990). “Studies on microbial leaching of heavy metals from municipal sludge.” Water Sci. Tech., 22(12), 229–238.
40.
Tyagi, R. D., Couillard, D., and Tran, F. T. (1991b). “Comparative study of bacterial leaching of metals from sewage sludge in continuous stirred tank and air‐lift reactors.” Proc. Biochem., 26, 47–54.
41.
Webber, M. D. (1988). “Contr⊚le de la concentration de métaux lourds dans les sols après épandage de boues d'égout municipales: l'approche canadienne.” Sci. Tech. Eau, 21(1), 45–51 (in French).
42.
Wong, L., and Henry, J. G. (1988). “Bacterial leaching of heavy metals from anaerobically digested sludge.” Biotreatment systems, D. L. Wise, ed., CRC Press Inc., Boca Raton, Fla., 125–169.
43.
Wozniak, D. J., and Huang, J. Y. C. (1982). “Variables affecting metal removal from sludge.” J. Water Pollut. Control Fed., 54(12), 1574–1580.

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Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 118Issue 5September 1992
Pages: 690 - 707

History

Published online: Sep 1, 1992
Published in print: Sep 1992

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Authors

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J. F. Blais
Assoc. Res., Université du Québec, Institut National de la Recherche Scientifique‐Eau, 2700 Rue Einstein, Sainte‐Foy, Quebec, Canada G1V 4C7
R. D. Tyagi
Prof. Université du Québec, Institut National de la Recherche Scientifique‐Eau, Sainte‐Foy, Quebec, Canada
J. C. Auclair
Prof. Université du Québec, Institut National de la Recherche Scientifique‐Eau, Sainte‐Foy, Quebec, Canada

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