INT‐Reduction Assays and Control of Sludge Bulking
Publication: Journal of Environmental Engineering
Volume 109, Issue 4
Abstract
Tetrazolium reduction assays, based on the reduction of 2‐(p‐iodophenyl)‐3‐(p‐nitrophenyl)‐5‐phenyl tetrazolium chloride (INT) to INT‐formazan by active bacterial electron transport systems, were used to differentiate between the physiological activities of filamentous and floc‐forming microorganisms in activated sludge following applications of chlorine. With proper dosage of chlorine, filaments (predominantly Sphaerotilus natans) were almost completely inactivated while floc‐forming microorganisms were only partially inhibited. Trends in the specific activity of S. atans anticipated similar trends in sludge volume index. Tetrazolium reduction assays were used in conjunction with a short term, static biassay to predict the chlorine dosage which controlled Sphaerotilus bulking in a bench scale, activated sludge system.
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Information & Authors
Information
Published In
Journal of Environmental Engineering
Volume 109 • Issue 4 • August 1983
Pages: 915 - 923
Copyright
Copyright © 1983 ASCE.
History
Published online: Aug 1, 1983
Published in print: Aug 1983
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