Technical Papers
Mar 28, 2024

Strength Investigation of Sand Treated by Enzyme-Induced Carbonate Precipitation Combined with Chitosan

Publication: International Journal of Geomechanics
Volume 24, Issue 6

Abstract

In this study, enzyme-induced carbonate precipitation (EICP) combined with chitosan curing technology was used to improve the mechanical properties of standard sand, and the curing effect of EICP combined with different chitosan contents was studied by macroscopic tests, such as the unconfined compressive strength test, direct shear test, and calcium carbonate content test, and microscopic tests, such as scanning electron microscope (SEM) and nuclear magnetic resonance (NMR). The results show that compared with the pure EICP treatment, the unconfined compressive strength, shear strength, and calcium carbonate content of the sand treated by EICP combined with chitosan were significantly improved, and increased first and then decreased with the increase of chitosan content, reaching the maximum value when the content is 1.5%. The calcium carbonate content is positively correlated with the strength, indicating that calcium carbonate crystals can effectively play a role in filling and cementation. After the incorporation of chitosan, the shape of calcium carbonate crystals is still mainly spherical, but the number and volume become larger. At the same time, the incorporation of chitosan can greatly reduce the proportion of large pores and medium pores, significantly increasing the proportion of small pores, which greatly improves the pore structure.

Get full access to this article

View all available purchase options and get full access to this article.

Data Availability Statement

The data used in this study are available from the corresponding author upon reasonable request.

Acknowledgments

This work was funded by the National Natural Science Foundation of China (No. 52268059) and the Jiangxi Provincial Natural Science Foundation General Project (No. 20232BAB204079).

References

Ahlafi, H., H. Moussout, F. Boukhlifi, M. Echetna, M. N. Bennani, and S. M. Slimane. 2014. “Kinetics of n-deacetylation of chitin extracted from shrimp shells collected from coastal area of Morocco.” Mediterr. J. Chem. 2: 503–513. https://doi.org/10.13171/mjc.2.3.2013.22.01.20.
Ayeldeen, M., A. Negm, M. El-Sawwaf, and M. Kitazume. 2017. “Enhancing mechanical behaviors of collapsible soil using two biopolymers.” J. Rock Mech. Geotech. Eng. 9 (2): 329–339. https://doi.org/10.1016/j.jrmge.2016.11.007.
Cheng, L., and M. A. Shahin. 2016. “Urease active bioslurry: A novel soil improvement approach based on microbially induced carbonate precipitation.” Can. Geotech. J. 53 (4): 1376–1385. https://doi.org/10.1139/cgj-2015-0635.
Cui, M., H. Xiong, J. Zheng, S. Lv, M. Cui, X. Fu, and S. Han. 2023. “Variable research on engineering characteristics of soybean urease reinforced sand.” KSCE J. Civ. Eng. 27 (8): 3313–3322. https://doi.org/10.1007/s12205-023-1959-y.
Cui, M.-J., H.-J. Lai, T. Hoang, and J. Chu. 2021. “One-phase-low-pH enzyme induced carbonate precipitation (EICP) method for soil improvement.” Acta Geotech. 16: 481–489. https://doi.org/10.1007/s11440-020-01043-2.
Cui, M.-J., H.-J. Lai, S.-F. Wu, and J. Chu. 2024. “Comparison of soil improvement methods using crude soybean enzyme, bacterial enzyme or bacteria-induced carbonate precipitation.” Géotechnique 74: 18–26. https://doi.org/10.1680/jgeot.21.00131.
Dilrukshi, R. A. N., and S. Kawasaki. 2019. “Effect of plant-derived urease-induced carbonate formation on the strength enhancement of sandy soil.” Ecol. Wisdom Inspired Restor. Eng. 5 (1): 93–108. https://doi.org/10.1007/978-981-13-0149-0_5.
Dong, J., and X. B. Liu. 2022. “Performance of traditional tabia improved by enzyme induced calcite precipitation technology.” J. Build. Mater. 25 (8): 853–859. https://doi.org/10.3969/j.issn.1007-9629.2022.08.012.
Gao, Y., J. He, X. Tang, and J. Chu. 2019. “Calcium carbonate precipitation catalyzed by soybean urease as an improvement method for fine-grained soil.” Soils Found. 59 (5): 1631–1637. https://doi.org/10.1016/j.sandf.2019.03.014.
Gao, Y.-F., H. Meng, J. He, Y.-S. Qi, and L. Hang. 2020. “Field trial on use of soybean crude extract for carbonate precipitation and wind erosion control of sandy soil.” J. Cent. South Univ. 27 (11): 3320–3333. https://doi.org/10.1007/s11771-020-4549-x.
GB (Guobiao Standards). 2019. Standard for geotechnical test method. GBT 50123-2019. Beijing: Planning Press.
GB (Guobiao Standards). 2021. Test method for strength of cement mortar (ISO method). GB/T 17671-2021. Beijing: Planning Press.
Hataf, N., P. Ghadir, and N. Ranjbar. 2018. “Investigation of soil stabilization using chitosan biopolymer.” J. Cleaner Prod. 170: 1493–1500. https://doi.org/10.1016/j.jclepro.2017.09.256.
Heidari, F., M. Razavi, M. E. Bahrololoom, R. Bazargan-Lari, D. Vashaee, H. Kotturi, and L. Tayebi. 2016. “Mechanical properties of natural chitosan/hydroxyapatite/magnetite nanocomposites for tissue engineering applications.” Mater. Sci. Eng., C 65: 338–344. https://doi.org/10.1016/j.msec.2016.04.039.
Imran, M. A., K. Nakashima, and S. Kawasaki. 2021. “Bio-mediated soil improvement using plant derived enzyme in addition to magnesium ion.” Crystals 11 (5): 516. https://doi.org/10.3390/cryst11050516.
Javadi, N., H. Khodadadi, N. Hamdan, and E. Kavazanjian. 2018. “EICP treatment of soil by using urease enzyme extracted from watermelon seeds.” In Vol. 296 of Proc., IFCEE 2018: Innovations in Ground Improvement for Soils, Pavements, and Subgrades, 115–124. Reston, VA: ASCE.
Kang, J., and R. A. Mclaughlin. 2020. “Polyacrylamide and chitosan biopolymer for flocculation and turbidity reduction in soil suspensions.” J. Polym. Environ. 28: 1335–1343. https://doi.org/10.1007/s10924-020-01682-2.
Khodadadi, T. H., N. Javadi, V. Krishnan, N. Hamdan, and E. Kavazanjian. 2020. “Crude urease extract for biocementation.” J. Mater. Civ. Eng. 32 (12): 04020374. https://doi.org/10.1061/(ASCE)MT.1943-5533.0003466.
Lai, H.-J., M.-J. Cui, and J. Chu. 2023. “Effect of pH on soil improvement using one-phase-low-pH MICP or EICP biocementation method.” Acta Geotech. 18 (6): 3259–3272. https://doi.org/10.1007/S11440-022-01759-3.
Lang, C. P., M. Ma, L. D. Qiu, Y. J. Yang, and M. D. Li. 2021. “Study on the improving effect of soybean urease induced calcium carbonate precipitation on the bearing capacity of sand ground: Based on results from static cone penetration tests.” Geol. J. China Univ. 27 (6): 784–788. https://doi.org/10.16108/j.issn1006-7493.2020212.
Lee, S., and J. Kim. 2020. “An experimental study on enzymatic-induced carbonate precipitation using yellow soybeans for soil stabilization.” KSCE J. Civ. Eng. 24 (7): 2026–2037. https://doi.org/10.1007/s12205-020-1659-9.
Li, S., C. Li, D. Yao, and S. Wang. 2020. “Feasibility of microbially induced carbonate precipitation and straw checkerboard barriers on desertification control and ecological restoration.” Ecol. Eng. 152: 105883. https://doi.org/10.1016/j.ecoleng.2020.105883.
Mohammadi, M., G. Habibagahi, and N. Hataf. 2021. “A bioinspired technique for improving the interaction between cohesive soil and geotextile reinforcements.” Int. J. Geosynth. Ground Eng. 7 (2): 34. https://doi.org/10.1007/s40891-021-00272-z.
Muhammed, A. S., K. A. Kassim, M. U. Zango, K. Ahmad, and J. Makinda. 2021. “Enhancing the strength of sandy soil through enzyme-induced calcite precipitation.” Int. J. Geosynth. Ground Eng. 7 (2): 45. https://doi.org/10.1007/s40891-021-00289-4.
Nam, I.-H., C.-M. Chon, K.-Y. Jung, S.-G. Choi, H. Choi, and S.-S. Park. 2015. “Calcite precipitation by ureolytic plant (Canavalia ensiformis) extracts as effective biomaterials.” KSCE J. Civ. Eng. 19 (6): 1620–1625. https://doi.org/10.1007/s12205-014-0558-3.
Orazzhanova, L. K., Z. S. Kassymova, B. K. Mussabayeva, and A. N. Klivenko. 2020. “Soil structuring in the presence of the chitosan–polyacrylic acid interpolymer complex.” Eurasian Soil Sci. 53: 1773–1781. https://doi.org/10.1134/S1064229320120091.
Park, S.-S., S.-G. Choi, and I.-H. Nam. 2014. “Effect of plant-induced calcite precipitation on the strength of sand.” J. Mater. Civ. Eng. 26: 06014017. https://doi.org/10.1061/(ASCE)MT.1943-5533.0001029.
Swain, K., M. Mahamaya, S. Alam, and S. K. Das. 2018. “Stabilization of dispersive soil using biopolymer.” Sustainable Civ. Infrastruct. 319: 132–147. https://doi.org/10.1007/978-3-319-61612-4_11.
Wei, L., S. X. Chai, Z. Liu, P. Wang, and F. Li. 2022. “Evaluation on compressive strength of fiber reinforced soil under freeze‒thaw cycles by scanning election microscopy and nuclear magnetic resonance.” Rock Soil Mech. 43 (S2): 163–170+182. https://doi.org/10.16285/j.rsm.2020.1739.
Whiffin, V. S. 2004. Microbial CaCO3 precipitation for the production of biocement. Perth West, Australia: Morduch Univ.
Wu, L. Y., L. C. Miao, X. H. Sun, R. F. Chen, and C. C. Wang. 2020. “Experimental study on sand solidification using plant-derived urease-induced calcium carbonate precipitation.” Chin. J. Geotech. Eng. 42 (4): 714–720. https://doi.org/10.11779/CJGE202004014.
Wu, L., L. Miao, X. Sun, and H. X. Wang. 2021. “Enzyme-Induced carbonate precipitation combined with polyvinyl alcohol to solidify Aeolian sand.” J. Mater. Civ. Eng. 33 (12): 04021373. https://doi.org/10.1061/(ASCE)MT.1943-5533.0004009.
Wu, Y. W., J. Hu, W. X. Zhang, S. C. Wang, and W. B. Liu. 2018. “Research status review of microbial induced carbonate precipitation technology in reinforcing sand.” Subgrade Eng. 15 (5): 6–11. https://doi.org/10.13379/j.issn.1003-8825.2018.05.02.
Xiao, Y., H. Chen, A. W. Stuedlein, T. M. Evans, J. Chu, L. Cheng, N. Jiang, H. Lin, H. Liu, and H. M. Aboel-Naga. 2020. “Restraint of particle breakage by biotreatment method.” J. Geotech. Geoenviron. Eng. 146 (11): 04020123. https://doi.org/10.1061/(ASCE)GT.1943-5606.0002384.
Xiao, Y., H. Li, J. Shi, J. Hu, L. Zhang, and H. Liu. 2023. “Effect of particle size on small strain stiffness of biotreated sands.” Transp. Geotech. 41: 101027. https://doi.org/10.1016/j.trgeo.2023.101027.
Yasuhara, H., D. Neupane, K. Hayashi, and M. Okamura. 2012. “Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation.” Soils Found. 52: 539–549. https://doi.org/10.1016/j.sandf.2012.05.011.
Zhang, J. W., Y. Han, H. L. Bian, X. S. Huang, X. J. Wang, and B. B. Li. 2020. “Experimental research on wind resistance of silty soil cemented by soybean urease induced calcium carbonate precipitation.” Ind. Constr. 50 (12): 19–24+118. https://doi.org/10.13204/j.gyjzG20021404.

Information & Authors

Information

Published In

Go to International Journal of Geomechanics
International Journal of Geomechanics
Volume 24Issue 6June 2024

History

Received: Aug 1, 2023
Accepted: Dec 19, 2023
Published online: Mar 28, 2024
Published in print: Jun 1, 2024
Discussion open until: Aug 28, 2024

Permissions

Request permissions for this article.

Authors

Affiliations

Associate Professor, School of Civil and Architectural Engineering, Nanchang Institute of Technology, Nanchang 330099, China; Jiangxi Provincial Engineering Research Center of the Special Reinforcement and Safety Monitoring Technology in Hydraulic and Civil Engineering, Nanchang 330099, China. Email: [email protected]
Huihui Xiong [email protected]
Postgraduate Researcher, School of Civil and Architectural Engineering, Nanchang Institute of Technology, Nanchang 330099, China (corresponding author). Email: [email protected]
Junjie Zheng [email protected]
Professor, School of Civil and Hydraulic Engineering, Huazhong Univ. of Science and Technology, Wuhan 430074, China. Email: [email protected]
Mingjuan Cui [email protected]
Associate Professor, College of Civil Engineering, Fuzhou Univ., Fuzhou 350116, China. Email: [email protected]
Postgraduate Researcher, Jiangsu Jianyuan Construction Co. Ltd., Suzhou 215000, China. Email: [email protected]
Shangyu Han [email protected]
Associate Professor, College of Civil Architecture, Nanchang Hangkong Univ., Nanchang 330063, China. Email: [email protected]

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share