Chapter
Apr 26, 2012
FISH-RiboSyn: A Rapid Approach for Determining the Specific Growth Rate of a Distinct Microbial Population in a Mixed Culture
Authors: Samuel J. DuPont, Matthew R. Cutter, and Peter G. Stroot [email protected]Author Affiliations
Publication: World Environmental and Water Resources Congress 2007: Restoring Our Natural Habitat
Abstract
Determining the specific growth rate of a distinct microbial population in a mixed culture or environmental sample has long been an elusive parameter which is integral to describing the function of microbes in their environment. FISH-RiboSyn, is an in situ method that utilizes fluorescence in situ hybridization (FISH) with specific primers that target the interior region of both pre16S rRNA and mature 16S rRNA. FISH-RiboSyn provides an expedient method for determining the specific growth rate of a distinct microbial population in a mixed culture sample. Preliminary results demonstrate the linear increasing nature of fluorescence intensity as a function of chloramphenicol exposure time in pure cultures of Acinetobacter calcoaceticus.
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World Environmental and Water Resources Congress 2007: Restoring Our Natural Habitat
Pages: 1 - 9
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© 2007 American Society of Civil Engineers.
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Published online: Apr 26, 2012
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Samuel J. DuPont
Department of Civil and Environmental Engineering, University of South Florida, 4202 East Fowler Avenue, ENB 118
Matthew R. Cutter
Department of Civil and Environmental Engineering, University of South Florida, 4202 East Fowler Avenue, ENB 118
Department of Civil and Environmental Engineering, University of South Florida, 4202 East Fowler Avenue, ENB 118. E-mail: [email protected]
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