A Theoritical Model Development — Effect of Initial Phenol Concentrations on Phenol Degradation by Using Pseudomonas putida Cells Immobilized on Calcium Alginate Matrix

Bio-kinetic model was developed for immobilized whole-cells using Briggs-Haldane equation incorporating the substrate inhibitory effects for Gaden Type — I fermentations. Model equations also include the effectiveness factor concept and the reaction contribution in liquid space as well as immobilization matrix, for exponential and equilibrium stage growth phases. The model equations were verified using batch experimental data obtained for a phenol degradation by Pseudomonas putida immobilized on calcium alginate matrix and the bio-kinetic parameters were determined. Batch immobilized whole-cells experimental data reveals that variation in the initial phenol concentration have significant effect on the time taken for degradation of phenol. Also effectiveness factor increases with increase in initial phenol concentration under exponential as well as equilibrium growth phases.