TECHNICAL PAPERS
Oct 1, 2004

Effect of Organic Loading Rate on Aerobic Granulation. I: Reactor Performance

This article has a reply.
VIEW THE REPLY
Publication: Journal of Environmental Engineering
Volume 130, Issue 10

Abstract

The effect of organic loading rate (OLR) on the aerobic granulation process was investigated using laboratory-scale sequential aerobic sludge blanket reactors (SASBRs). Reactors R1, R2, R3, and R4 were operated at OLRs of 1, 2, 4 and 8kg chemical oxygen demand (COD)/m3day, respectively. Aerobic granules could not be formed at the relatively low OLRs in R1 and R2. Stable aerobic granules were successfully cultivated at the mid-range OLR of 4kg COD/m3day tested in Reactor R3. These granules first appeared 14 days after startup and eventually grew to become the dominant form of biomass in R3. The granular biomass stabilized at a mixed liquor volatile suspended solids (MLVSS) concentration of 12,000mg/L, with a food-to-microorganism (F/M) ratio of 0.33kg COD/kg MLVSSday and a mean cell residence time of 31.1 days. Aerobic granules were first observed on Day 18 in Reactor R4, which operated at the highest OLR tested of 8kg COD/m3day. However, these granules were unstable and eventually washed out of R4. The best reactor performance was achieved in R3 with a COD removal rate of 99%, an observed yield coefficient (YOB) of 0.10mg MLVSS/mg COD, and a sludge volume index 24mL/g MLVSS. The volumetric specific oxygen utilization rate was highest in R3, at 356mg O2/Lh. An optimal choice of OLR was found to favor the cultivation and retention of well-settling granules and enhanced the overall ability of the reactor to remove COD. This study contributes to a better understanding of the role of OLR in aerobic granulation.

Get full access to this article

View all available purchase options and get full access to this article.

References

1.
American Public Health Association (APHA). (1998). Standard methods for the examination of water and wastewater, 20th Ed., Washington, D.C.
2.
Barbusinski, K., and Koscielniak, H. (1995). “Influence of substrate loading intensity on floc size in activated sludge process.” Water Res., 29(7), 1703–1710.
3.
Beeftink, H.H. ( 1987). “Anaerobic bacterial aggregates.” PhD thesis, Univ. of Amsterdam, Amsterdam, The Netherlands.
4.
Beun, J. J., Hendriks A., van Loodrecht, M. C. M., Morgenroth, E., Wilderer, P. A., and Heijnen, J. J. (1999). “Aerobic granulation in a sequencing batch reactor.” Water Res., 33(10), 2283–2290.
5.
Bossier, P., and Verstraete, W. (1996). “Triggers for microbial aggregation in activated sludge?” Appl. Microbiol. Biotechnol. 45, 1–6.
6.
Chang, H. T., Rittmann, B. E., Amar, D., Heim, R., Ehrlinger, O., and Lesty, Y. (1991). “Biofilm detachment mechanisms in a liquid fluidized bed.” Biotechnol. Bioeng., 38, 499–506.
7.
de Beer, D., van der Heuvel, J. C., and Oftengraf, S. P. P. (1993). “Microelectrode measurements in nitrifying aggregates.” Appl. Environ. Microbiol., 59, 573–579.
8.
Fukuzaki, S., Chang, Y. J., Nishio, N., and Nagai, S. (1991) “Characteristics of granule methanogenic sludge grown on lactate in an UASB reactor.” J. Ferment. Bioeng., 72(6), 465–l72.
9.
Heijinen, J. J., van Loosdrecht, M. C. M., Mulder, A., and Tijhuis, L. (1992). “Formation of biofilms in a bioflim airlift suspension reactor.” Water Sci. Technol., 26, 647–654.
10.
Hulshoff Pol, L. W., de Zeeuw, W. J., Velzeboer, C. T. M., and Lettinga, G. (1983). “Granulation in UASB reactors.” Water Sci. Technol., 15, 291–304.
11.
Jiang, H. L., Tay, J. H., and Tay, S. T. L. (2002). “Aggregation of immobilized activated sludge cells into aerobically grown microbial granules for the aerobic biodegradation of phenol.” Lett. Appl. Microbiol., 35, 439–45.
12.
Kugaprasatham, S., Nagaoka, H., and Ohgaki, S. (1992). “Effect of turbulence on nitrifying biofilm at non-limiting substrate conditions.” Water Res., 12, 1629–1638.
13.
Kwok, W. K., Picioreanu, C., Ong, S. L., van Loosdrecht, M. C. M., Ng,W. J., and Heijnen, J. J. (1998). “Influence of biomass production and detachment forces on biofilm structures in a biofilm airlift suspension reactor.” Biotechnol. Bioeng., 58, 400–407.
14.
Lettinga, G., Field, J., Lier, J., van Zeeman, G., and Hulshoff, Pol L. W. (1997). “Advanced anaerobic wastewater treatment in the near future.” Water Sci. Technol., 35(10), 5–12.
15.
Lettinga, G., van Velsen, A. F. M., Hobma, S. W., De Zeeuw, W., and Klapwijk, A. (1980). “Use of the upflow sludge blanket (USB) reactor concept for biological wastewater treatment, especially for anaerobic treatment.” Biotechnol. Bioeng., 22, 699–734.
16.
Li, D., and Ganczarczyk, J. J. (1988) “Flow through activated sludge flocs.” Water Res., 22, 789–792.
17.
Liao, B. Q., Allen, D. G., Droppo, I. G., Leppard, G. G., and Liss, S. N. (2001). “Surface properties of sludge and their role in bioflocculation and settleability.” Water Res., 35(2), 339–350.
18.
Magara, Y., Nambu, S., and Utosawa, K. (1976). “Biochemical and physical properties of activated–sludge on settling characteristics.” Water Res., 10, 71–77.
19.
Matsumoto A., and Noike T. (1991). “Effects of substrate composition and loading rate on methanogenic process in anaerobic fluidized bed systems.” Water Sci. Technol., 23, 1311–1317.
20.
Metcalf and Eddy, Inc. (1991). Wastewater engineering: Treatment, disposal, and reuse. 3rd rev. Ed., G. Tchobanoglous and F. L. Burton, eds. McGraw-Hill, New York.
21.
Mishima, K., and Nakamura, M. (1991). “Self-immobilization of aerobic activated sludge—A pilot study of the aerobic upflow sludge blanket process in municipal sewage treatment.” Water Sci. Technol., 23, 981–990.
22.
Morgan, J. W., Evison, L. M., and Forster, C. F. (1991). “Upflow sludge blanket reactors: The effect of biosupplements on performance and granulation.” J. Chem. Technol. Biotechnol., 52, 243–255.
23.
Morgenroth, E., Sherden T., van Loosdrecht, M. C. M., Heijnen, J. J., and Wilderer, P. A. (1997). “Aerobic granulation sludge in a sequencing batch reactor.” Water Res., 31(12), 3191–3194.
24.
Moy, B. Y. P., Tay, J. H., Toh, S. K., Liu, L., and Tay, S. T. L. (2002). “High organic loading influences the physical characteristics of aerobic sludge granules.” Lett. Appl. Microbiol., 34, 1–6.
25.
Námer, J., and Ganczarczyk, J. J. (1993). “Settling properties of digested sludge aggregates.” Water Res., 27, 1285–1294.
26.
Parker, D. S., Kaufman, W. J., and Jenkins, D. (1971). “Physical conditioning of the activated sludge floc.” J. Water Pollut. Control Fed., 43, 1817–1833.
27.
Rittman, B. E., and McCarty, P. L. (1980). “Model of steady-state-biofilin kinetics.” Biotechnol. Bioeng., 22, 2243–2357.
28.
Sezgin, M., Jenkins, D., and Parker, D. S. (1978). “A united theory of filamentous activated sludge bulking.” J. Water Pollut. Control Fed., 50, 362–381.
29.
Shin, H. S., Lim, K. H., and Park, H. S. (1992). “Effect of shear stress on granulation in oxygen aerobic upflow sludge reactors.” Water Sci. Technol., 26(3–4), 601–605.
30.
Takahashi, M., and Kyosai, S. (1991). “Pilot plant study on microaerobic self-granuled sludge process (multi-stage reversing flow bioreactor: MBR).” Water Sci. Technol., 23, 973–980.
31.
Tay, J. H., and Yan, Y. G. (1996). “Influence of substrate concentration on microbial selection and granulation during start-up of upflow anaerobic sludge blanket reactors.” Water Environ. Res., 68(7), 1140–1150.
32.
Tay, S. T. L., Ivanov, V., Yi, S., Zhuang, W. Q., and Tay., J. H. (2002). “Presence of anaerobic Bacteroides in aerobically grown microbial granules.” Microb. Ecol., 44(3), 278–285.
33.
Tay, J. H., Liu, Q. S., and Liu, Y. (2001). “The effects of shear force on the formation, structure and metabolism of aerobic granules.” Appl. Microbiol. Biotechnol. 57(1), 227.
34.
Tay, J.-H., Pan, S., He, Y., and Tay, S. T. L. (2004). “Effect of organic loading rate on aerobic granulation. II: Characteristics of aerobic granules.” J. Environ. Eng., 130(10), 1102–1109.
35.
Tijhuis, L., Hijmanman, B., van Loosdrecht, M. C. M., and Heijnen, J. J. (1996). “Influence of detachment, substrate loading and reactor scale on the formation of biofilnis in airlift reactors.” Appl. Microbiol. Biotechnol. 45, 7–17.
36.
Tijhuis, L., van Loosdrecht, M. C. M., and Heijnen, J. J. (1994). “Formation and growth of heterotrophic aerobic bioflims on small suspended particles in airlift reactors.” Biotechnol. Bioeng., 44, 595–608.
37.
Urbain, V., Block, J. C., and Manem, J. (1993). “Bioflocculation in activated-sludge: an analytic approach.” Water Res., 27(5), 829–838.
38.
van Benthum, W. A. J., Garrido-Fernández, J. M., Tijhuis, L., van Loosdrecht, M. C. M., and Heijnen, J. J. (1996). “Formation and detachment of biofilin and granules in a nitrifying airlift suspension reactor.” Biotechnol. Prog., 12, 764–772.
39.
van der Hoek, J.P. ( 1988). “Granulation of denitrifying sludge.” Granular anaerobic sludge, A. Lettinga et al., eds., Pol. Pudoc, Wageningen, The Netherlands, 203–210.
40.
van Loosdrecht, M. C. M., Tijhuis, L., Wihdieks, A. M. S., and Heijnen, J. J. (1995). “Population distribution in aerobic bioflims on small suspended particles.” Water Sci. Technol., 31, 163–171.
41.
Viera, M. J., Melo, L. F., and Pinheiro, M. M. (1993). “Bioflim formation: hydrodynamic effects on internal diffusion and structure.” Biofouling, 7, 67–80.
42.
Visser, F. A., van Lier, J. B., Macano, A. J. L., and de Macario, E. C. (1991). “Diversity and population dynamics of methanogenic bacteria in a granular consortium.” Appl. Environ. Microbiol., 57, 1728–1734.
43.
Watanabe, K., Miyashita, M., and Harayama, S. (2000). “Starvation improves survival of bacteria introduced into activated sludge.” Appl. Environ. Microbiol. 66, 3905–3910.
44.
Yan, Y. G. and Tay, J. H. (1997). “Characterisation of the granulation process during UASB start-up.” Water Res. 31(7), 1573–1580.
45.
Zahid, W. M., and Ganczarczyk, J. J. (1990). “Suspended solids in biological filter effluents.” Water Res., 24, 215–220.

Information & Authors

Information

Published In

Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 130Issue 10October 2004
Pages: 1094 - 1101

History

Published online: Oct 1, 2004
Published in print: Oct 2004

Permissions

Request permissions for this article.

Authors

Affiliations

Joo-Hwa Tay
Professor and Head, Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technical Univ., 50 Nanyang Ave., Singapore 639798.
Shun Pan
Project Officer, Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technical Univ., 50 Nanyang Ave., Singapore 639798.
Yanxin He
Research Associate, Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technical Univ., 50 Nanyang Ave., Singapore 639798.
Stephen Tiong Lee Tay
Associate Professor, Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technical Univ., 50 Nanyang Ave., Singapore 639798.

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share