Technical Papers
May 6, 2019

Effect of Organic Matter Content on Enzymatic Biocementation Process Applied to Coarse-Grained Soils

Publication: Journal of Materials in Civil Engineering
Volume 31, Issue 7

Abstract

This work studies the effect of the organic matter content on the efficiency of the biocementation process due to the precipitation of calcium carbonate (CaCO3) induced by the urease enzyme. Eight different mixtures of an organic soil and a clayed soil are analyzed, which correspond to a range of organic matter content from 0% to 11.3%. The impact of the biocementation process is examined in terms of the strength and stiffness obtained from unconfined compressive strength tests and complemented by measuring the amount of CaCO3 precipitated, pH value, X-ray diffraction (XRD), and scanning electron microscopy (SEM) tests with energy dispersive X-ray (EDX). In general, the biocementation induces an increase in the strength of the material and an increase in the brittleness of the stress-strain behavior. The results also show that the strength gain due to biocementation increases with the amount of CaCO3 precipitate, with greater significance when the content of CaCO3 is lower than 0.5%. The XRD and SEM/EDX tests are coherent with the amount of CaCO3, and both confirm the existence of the precipitation of CaCO3.

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Acknowledgments

This work was supported by FCT (Portuguese Foundation for Science and Technology), within ISISE, project UID/ECI/04029/2013.

References

Al Qabany, A., and K. Soga. 2013. “Effect of chemical treatment used in MICP on engineering properties of cemented soils.” Géotechnique 63 (4): 331–339. https://doi.org/10.1680/geot.SIP13.P.022.
Al-Thawadi, S. M. 2013. “Consolidation of sand particles by aggregates of calcite nanoparticles synthesized by ureolytic bacteria under non-sterile conditions.” J. Chem. Sci. Technol. 2 (3): 141–146.
ASTM. 2000. Standard practice for classification of soils for engineering purposes (unified soil classification system). ASTM D2487. West Conshohocken, PA: ASTM.
ASTM. 2005. Standard test method for unconfined compressive strength of cohesive soil. ASTM D2166. West Conshohocken, PA: ASTM.
Blakeley, R. L., and B. Zerner. 1984. “Jack bean urease: The first nickel enzyme.” J. Mol. Catal. 23 (2–3): 263–292. https://doi.org/10.1016/0304-5102(84)80014-0.
BSI (British Standards Institution). 1990. Methods of test for soils for civil engineering purposes: Chemical and electro-chemical testing. BS 1377-3. London: BSI.
Burbank, M., T. Weaver, T. Green, B. Williams, and R. Crawford. 2011. “Precipitation of calcite by indigenous microorganisms to strengthen liquefiable soils.” Geomicrobiol. J. 28 (4): 301–312. https://doi.org/10.1080/01490451.2010.499929.
Burbank, M., T. Weaver, R. Lewis, T. Williams, B. Williams, and R. Crawford. 2013. “Geotechnical tests of sands following bioinduced calcite precipitation catalyzed by indigenous bacteria.” J. Geotech. Geoenviron. Eng. 139 (6): 928–936. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000781.
Carmona, J. P. S. F., P. J. Venda Oliveira, L. J. L. Lemos, and A. M. G. Pedro. 2018. “Improvement of a sandy soil by enzymatic calcium carbonate precipitation.” ICE Geotech. Eng. 171 (1): 3–15. https://doi.org/10.1680/jgeen.16.00138.
CEN (European Committee for Standardization). 2000. Concrete. Part I: Specification, performance, production and conformity. EN 206-1. Brussels, Belgium: CEN.
Cheng, L., R. Cord-Ruwisch, and M. A. Shahin. 2013. “Cementation of sand soil by microbially induced calcite precipitation at various degrees of saturation.” Can. Geotech. J. 50 (1): 81–90. https://doi.org/10.1139/cgj-2012-0023.
Cheng, L., M. A. Shahin, and R. Cord-Ruwisch. 2014. “Bio-cementation of sandy soil using microbially induced carbonate precipitation for marine environments.” Géotechnique 64 (12): 1010–1013. https://doi.org/10.1680/geot.14.T.025.
Chou, C. W., E. A. Seagren, A. H. Aydilek, and M. Lai. 2011. “Biocalcification of sand through ureolysis.” J. Geotech. Geoenviron. Eng. 132 (17): 1179–1189. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000532.
Chu, J., V. Stabnikov, and V. Ivanov. 2012. “Microbially induced calcium carbonate precipitation on surface or in the bulk of soil.” Geomicrobiol. J. 29 (6): 544–549. https://doi.org/10.1080/01490451.2011.592929.
Correia, A. A. S. 2011. “Applicability of deep mixing technique to the soft soil of Baixo Mondego.” Ph.D. dissertation, Dept. of Civil Engineering, Univ. of Coimbra.
Dejong, J. T., B. M. Mortensen, B. C. Martinez, and D. C. Nelson. 2010. “Bio-mediated soil improvement.” Ecol. Eng. 36 (2): 197–210. https://doi.org/10.1016/j.ecoleng.2008.12.029.
DeJong, J. T., B. C. Martinez, T. R. Ginn, C. Hunt, D. Major, and B. Tanyu. 2014. “Development of a scaled repeated five-spot treatment model for examining microbial induced calcite precipitation feasibility in field applications.” Geotech. Test. J. 37 (3): 20130089. https://doi.org/10.1520/GTJ20130089.
Gomez, M. G., S. M. Dworatzek, B. C. Martinez, L. A. deVlaming, J. T. DeJong, C. E. Hunt, and D. W. Major. 2015. “Field-scale bio-cementation tests to improve sands.” Proc. Inst. Civ. Eng. Ground Improv. 168 (3): 206–216. https://doi.org/10.1680/grim.13.00052.
Hammes, F., and W. Verstraete. 2002. “Key roles of pH and calcium metabolism in microbial carbonate precipitation.” Rev. Environ. Sci Biotechnol. 1 (1): 3–7. https://doi.org/10.1023/A:1015135629155.
Ismail, M. A., H. A. Joer, M. F. Randolph, and A. Merit. 2002. “Cementation of porous materials using calcite.” Géotechnique 52 (5): 313–324. https://doi.org/10.1680/geot.52.5.313.38709.
Jiang, N. J., and K. Soga. 2016. “The applicability of microbially induced calcite precipitation (MICP) for internal erosion control in gravel-sand mixtures.” Geotechnique 67 (1): 42–55. https://doi.org/10.1680/jgeot.15.P.182.
Kalantary, F., and M. Kahani. 2015. “Evaluation of the ability to control biological precipitation to improve sandy soils.” Procedia Earth Planet. Sci. 15: 278–284. https://doi.org/10.1016/j.proeps.2015.08.067.
Li, W., W. S. Chen, P. P. Zhou, and L. J. Yu. 2013. “Influence of enzyme concentration on bio-sequestration of CO2 in carbonate form using bacterial carbonic anhydrase.” Chem. Eng. J. 232 (Oct): 149–156. https://doi.org/10.1016/j.cej.2013.07.069.
Lin, H., M. T. Suleiman, D. G. Brown, and E. Kavazanjian Jr. 2016. “Mechanical behavior of sands treated by microbially induced carbonate precipitation.” J. Geotech. Geoenviron. Eng. 142 (2): 04015066. https://doi.org/10.1061/(ASCE)GT.1943-5606.0001383.
Mahawish, A., A. Bouazza, and W. P. Gates. 2016. “Biogrouting coarse materials using soil-lift treatment strategy.” Can. Geotech. J. 53 (12): 2080–2085. https://doi.org/10.1139/cgj-2016-0167.
Montoya, B., J. DeJong, R. Boulanger, D. Wilson, R. Gerhard, A. Ganchenko, and J. Chou. 2012. “Liquefaction mitigation using microbial induced calcite precipitation.” In Proc., GeoCongress, 1918–1927. Reston, VA: ASCE.
Mortensen, B. M., M. J. Haber, J. T. DeJong, L. F. Caslake, and D. C. Nelson. 2011. “Effects of environmental factors on microbial induced calcium carbonate precipitation.” J. Appl. Microbiol. 111 (2): 338–349. https://doi.org/10.1111/j.1365-2672.2011.05065.x.
Mujah, D., and L. Cheng. 2016. “State-of-the-art review of biocementation by microbially induced calcite precipitation (MICP) dor soil stabilization.” Geomicrobiol. J. 34 (6): 524–537. https://doi.org/10.1080/01490451.2016.1225866.
Nemati, M., and G. Voordouw. 2003. “Modification of porous media permeability, using calcium carbonate produced enzymatically in situ.” Enzyme Microb. Technol. 33 (5): 635–642. https://doi.org/10.1016/S0141-0229(03)00191-1.
Neupane, D., H. Yasuhara, and N. Kinoshita. 2015a. “Evaluation of enzyme mediated calcite grouting as a possible improvement technique.” In Computer methods and recent advances in geomechanics, 1169–1172. London: Taylor & Francis.
Neupane, D., H. Yasuhara, N. Kinoshita, and T. Unno. 2013. “Applicability of enzymatic calcium carbonate precipitation as a soil-strengthening technique.” J. Geotech. Geoenviron. Eng. 139 (12): 2201–2211. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000959.
Neupane, D., H. Yasuhara, N. Kinoshita, and T. Unno. 2015b. “Distribution of mineralized carbonate and its quantification method in enzyme mediated calcite precipitation technique.” Soil Found. 55 (2): 447–457. https://doi.org/10.1016/j.sandf.2015.02.018.
Neves, J. P. G. 2018. Improvement of soils with enzymes. Analysis of the effect of the organic matter content. M.Sc. dissertation, Dept. of Civil Engineering, Univ. of Coimbra.
Rebata-Landa, V. 2007. Microbial activity in sediments: Effects on soil behavior. Ph.D. dissertation, School of Civil and Environmental Engineering, Georgia Institute of Technology.
Rebata-Landa, V., and J. C. Santamarina. 2006. “Mechanical limits to microbial activity in deep sediments.” Geochem. Geophys. Geosyst. 78 (11): 1–12. https://doi.org/10.1029/2006GC001355.
Stocks-Fisher, S., J. K. Galinat, and S. S. Bang. 1999. “Microbiological precipitation of CaCO3.” Soil Biol. Biochem. 31 (11): 1563–1571.
van Paassen, L. 2009. Biogrout: Ground improvement by microbially induced carbonate precipitation. Ph.D. thesis, Dept. of Biotechnology, Delft Univ. of Technology.
Van Paassen, L. A., R. Ghose, T. J. M. van der Linden, W. R. L. van der Star, and M. C. M. van Loosdrecht. 2010a. “Potential soil reinforcement by biological denitrification.” Ecol. Eng. 36 (2): 1721–1728. https://doi.org/10.1016/j.ecoleng.2009.03.026.
Van Paassen, L. A., R. Ghose, T. J. M. van der Linden, W. R. L. van der Star, and M. C. M. van Loosdrecht. 2010b. “Quantifying biomediated ground improvement by ureolysis: Large-scale biogrout experiment.” J. Geotech. Geoenviron. Eng. 136 (12): 1721–1728. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000382.
Venda Oliveira, P. J., A. A. S. Correia, and T. J. S. Lopes. 2014. “Effect of organic matter content and binder quantity on the uniaxial creep behaviour of an artificially stabilized soil.” J. Geotech. Geoenviron. Eng. 140 (9): 04014053. https://doi.org/10.1061/(ASCE)GT.1943-5606.0001158.
Venda Oliveira, P. J., A. A. S. Correia, J. M. N. P. C. Teles, and D. G. Custódio. 2016a. “Effect of fibre type on the compressive and tensile strength of a soft soil chemically stabilized.” Geosynth. Int. 23 (3): 171–182. https://doi.org/10.1680/jgein.15.00040.
Venda Oliveira, P. J., M. S. Costa, J. N. P. Costa, and M. F. Nobre. 2015. “Comparison of the ability of two bacteria to improve the behaviour of a sandy soil.” J. Mater. Civ. Eng. 27 (1): 06014025. https://doi.org/10.1061/(ASCE)MT.1943-5533.0001138.
Venda Oliveira, P. J., L. D. Freitas, and J. P. S. F. Carmona. 2016b. “Effect of soil type on the enzymatic calcium carbonate precipitation process used for soil improvement.” J. Mater. Civ. Eng. 29 (4): 04016263. https://doi.org/10.1061/(ASCE)MT.1943-5533.0001804.
Venda Oliveira, P. J., A. F. V. Vieira, and A. A. S. Correia. 2017. “Effect of organic matter content on creep mitigation by preloading.” ICE Geotech. Eng. 170 (4): 305–311. https://doi.org/10.1680/jgeen.16.00082.
Whiffin, V. S., L. A. van Paassen, and M. P. Harkes. 2007. “Microbial carbonate precipitation as a soil improvement technique.” Geomicrobiol. J. 24 (5): 417–423. https://doi.org/10.1080/01490450701436505.
Yasuhara, H., D. Neupane, K. Hayashi, and M. Okamura. 2012. “Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation.” Soil Found. 52 (3): 539–549. https://doi.org/10.1016/j.sandf.2012.05.011.

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Go to Journal of Materials in Civil Engineering
Journal of Materials in Civil Engineering
Volume 31Issue 7July 2019

History

Received: Jun 19, 2018
Accepted: Jan 30, 2019
Published online: May 6, 2019
Published in print: Jul 1, 2019
Discussion open until: Oct 6, 2019

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Paulo J. Venda Oliveira [email protected]
Associated Professor, Dept. of Civil Engineering, Univ. of Coimbra, R. Luís Reis Santos, 3030-788 Coimbra, Portugal (corresponding author). Email: [email protected]
João P. G. Neves [email protected]
Master’s Student, Dept. of Civil Engineering, Univ. of Coimbra, R. Luís Reis Santos, 3030-788 Coimbra, Portugal. Email: [email protected]

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