Comparison of the Ability of Two Bacteria to Improve the Behavior of Sandy Soil
Publication: Journal of Materials in Civil Engineering
Volume 27, Issue 1
Abstract
This paper presents a laboratory study of the ability of two bacteria to improve the geomechanical properties of a sandy soil by calcium carbonate precipitation. The performance of two bacteria (Sporosarcina pasteurii and Idiomarina insulisalsae) is compared, based on unconfined compressive strength and splitting tensile strength tests. The effects of curing time and the concentration of I. insulisalsae on the strengthening process are also analyzed. Although the optimum environmental conditions for the bacterial growth were not adopted, the potential to improve the geomechanical properties of natural sandy soils is shown by the results. The bacteria I. insuliasalsae is shown to be more efficient than S. pasteurii in strengthening the soil, and increases in the concentration of I. isuliasalsae induce an increase in the geomechanical properties. However, this effect is not proportional to the amount of bacteria, probably due to a lack of nutrient.
Get full access to this article
View all available purchase options and get full access to this article.
Acknowledgments
The authors would like to express their gratitude to the institutions that financially supported the research: CIEC, CICC, and FCT (PTDC/ECM/101875/2008).
References
Al Qabany, A., and Soga, K. (2013). “Effect of chemical treatment used in MICP on engineering properties of cemented soils.” Géotechnique, 63(4), 331–339.
ASTM. (2000). “Standard practice for classification of soils for engineering purposes (unified soil classification system).”, West Conshohocken, PA.
ASTM. (2003). “Standard test methods for laboratory compaction characteristics of soil using standard effort [ ()].”, West Conshohocken, PA.
ASTM. (2005a). “Standard test method for splitting tensile strength of intact rock core specimens.”, West Conshohocken, PA.
ASTM. (2005b). “Standard test method for unconfined compressive strength of cohesive soil.”, West Conshohocken, PA.
Borgne, S. L., Paniagua, D., and Duhalt, R. V. (2008). “Biodegradation of organic pollutants by halophilic bacteria and Archaea.” J. Mol. Microbiol. Biotechnol., 15(2–3), 74–92.
British Standards Institution (BSI). (1990). “Methods of test for soils for civil engineering purposes—Part 3: Chemical and electro-chemical tests.”, London.
Burbank, M., Weaver, T., Green, T., Williams, B., and Crawford, R. (2011). “Precipitation of calcite by indigenous microorganisms to strengthen liquefiable soils.” Geomicrobiol. J., 28(4), 301–312.
Burbank, M., Weaver, T., Lewis, R., Williams, T., Williams, B., and Crawford, R. (2013). “Geotechnical tests of sands following bioinduced calcite precipitation catalyzed by indigenous bacteria.” J. Geotech. Geoenviron. Eng., 928–936.
Chaturvedi, S., Chandra, R., and Rai, V. (2006). “Isolation and characterization of phragmites australis (L.) rhizosphere bacteria from contaminated site for bioremediation of colored distillery effluent.” Ecol. Eng., 27(3), 202–207.
Cheng, L., Cord-Ruwisch, R., and Shahin, M. A. (2013). “Cementation of sand soil by microbially induced calcite precipitation at various degrees of saturation.” Can. Geotech. J., 50(1), 81–90.
Chou, C. W., Seagren, E. A., Aydilek, A. H., and Lai, M. (2011). “Biocalcification of sand through ureolysis.” J. Geotech. Geoenviron. Eng., 1179–1189.
Chu, J., Stabnikov, V., and Ivanov, V. (2012). “Microbially induced calcium carbonate precipitation on surface or in the bulk of soil.” Geomicrobiol. J., 29(6), 544–549.
Costa, J. N. P. (2012). “Biological and chemical stabilization of soils.” M. Sc. dissertation, Univ. of Coimbra, Coimbra, Portugal (in Portuguese).
DeJong, J., Fritzges, M., and Nüsslein, K. (2006). “Microbially induced cementation to control sand response to undrained shear.” J. Geotech. Geoenviron. Eng., 1381–1392.
DeJong, J. T., Mortensen, B. M., Martinez, B. C., and Nelson, D. C. (2010). “Bio-mediated soil improvement.” Ecol. Eng., 36(2), 197–210.
Hammes, F., Seka, A., Knijf, S., and Verstraete, W. (2003). “A novel approach to calcium removal from calcium-rich industrial wastewater.” Water Res., 37(3), 699–704.
Hammes, F., and Verstraete, W. (2002). “Key roles of pH and calcium metabolism in microbial carbonate precipitation.” Rev. Environ. Sci. Biotechnol., 1(1), 3–7.
Inagaki, Y., Tsukamoto, M., Mori, H., Nkajima, S., Ssaki, T., and Kawasaki, S. (2011). “A centrifugal model test of microbial carbonate precipitation as liquefaction countermeasure.” Jpn. Geotech. J., 6(2), 157–167 (in Japanese).
Jroundi, F., Suaga, P. G., Lopez, C. J., Muñoz, M. T. G., and Vivas, M. A. F. (2012). “Stone-isolated carbonatogenic bacteria as inoculants in bioconsolidation treatments for historical limestone.” Sci. Total Environ., 425, 89–98.
Kim, H. K., Park, S. J., Han, J. I., and Lee, H. K. (2013). “Microbially mediated calcium carbonate precipitation on normal and lightweight concrete.” Constr. Build. Mater., 38, 1073–1082.
Lee, Y. N. (2003). “Calcite production by Bacillus amyloliquefaciens CMB01.” J. Microbiol., 41(4), 345–348.
Montoya, B., et al. (2012). “Liquefaction mitigation using microbial induced calcite precipitation.” GeoCongress 2012, Geo-Institute, ASCE, 1918–1927.
Mortensen, B. M., Haber, M. J., DeJong, J. T., Caslake, L. F., and Nelson, D. C. (2011). “Effects of environmental factors on microbial induced calcium carbonate precipitation.” J. Appl. Microbiol., 111(2), 338–349.
Muynck, W. D., Belie, N. D., and Verstraete, W. (2010). “Microbial carbonate precipitation in construction materials: A review.” Ecol. Eng., 36(2), 118–136.
Nemati, M., Greene, E. A., and Voordouw, G. (2005). “Permeability profile modification using bacterially formed calcium carbonate: Comparison with enzymic option.” Process Biochem., 40(2), 925–933.
Rong, H., Qian, C., and Li, L. (2012). “Influence of molding process on mechanical properties of sandstone cemented by microbe cement.” Constr. Build. Mater., 28(1), 238–243.
Shirakawa, M. A., Kaminishikawahara, K. K., John, V. M., Kahn, H., and Futai, M. M. (2011). “Sand bioconsolidation through the precipitation of calcium carbonate by two ureolytic bacteria.” Mater. Lett., 65(11), 1730–1733.
Taborda, M., Antunes, A., Tiago, I., Veríssimo, A., Nobre, M. F., and Costa, M. S. (2009). “Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina.” Syst. Appl. Microbiol., 32(6), 371–378.
Tittelboom, K. V., Belie, N. D., Muynck, W. D., and Verstraete, W. (2010). “Use of bacteria to repair cracks in concrete.” Cem. Concr. Res., 40(1), 157–166.
van Paassen, L. A., Ghose, R., van der Linden, T. J. M., van der Star, W. R. L., and van Loosdrecht, M. C. M. (2010). “Quantifying biomediated ground improvement by ureolysis: Large-scale biogrout experiment.” J. Geotech. Geoenviron. Eng., 1721–1728.
Webster, A., and May, E. (2006). “Bioremediation of weathered-building stone surfaces.” Trends Biotechnol., 24(6), 255–260.
Whiffin, V. S., van Paassen, L. A., and Harkes, M. P. (2007). “Microbial carbonate precipitation as a soil improvement technique.” Geomicrobiol. J., 24(5), 417–423.
Yoon, J. H., Lee, K. C., Weiss, N., Kho, Y. H., Kang, K. H., and Park, Y. H. (2001). “Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb. nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of the genus Sporosarcina.” Int. J. Syst. Evol. Microbiol., 51(3), 1079–1086.
Information & Authors
Information
Published In
Copyright
© 2014 American Society of Civil Engineers.
History
Received: Feb 26, 2014
Accepted: Jun 5, 2014
Published online: Jul 9, 2014
Discussion open until: Dec 9, 2014
Published in print: Jan 1, 2015
Authors
Metrics & Citations
Metrics
Citations
Download citation
If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.