Technical Papers
Jan 23, 2019

Impact of Organic Loading Rate and Earthworms on Dissolved Oxygen and Vermifiltration

Publication: Journal of Hazardous, Toxic, and Radioactive Waste
Volume 23, Issue 2

Abstract

In order to facilitate an affordable, sustainable, and technologically viable alternative to traditional brewery wastewater remediation technologies, vermifiltration was explored in this study. The impacts of high organic loading rates (OLRs) on removal of organics and nutrients from a vermifilter and their relationship with earthworm inoculation rates were the principal focus. The current study also investigated the role of dissolved oxygen (DO) in vermifiltration. To conduct this study, OLRs ranged 2.2511.25kgCOD/m3·d, while earthworm numbers varied 010,000  perm3. The average effluent DO was in the range 0.80.9  mg/L from biofilters and 1.52.1  mg/L from vermifilters. A chemical oxygen demand (COD) removal efficiency of 92%–96% and a NH4+-N conversion efficiency of 81%–85% were observed when OLRs ranged 2.253.15kgCOD/m3·d and earthworm density (EWD) ranged 5,00010,000·NO3-N generation was also observed to be in parity with NH4+-N conversion from the system. The impact of DO on COD removal, nitrification, and denitrification was found to be significant. A maximum total nitrogen (TN) removal efficiency of 24% was observed at an EWD of 10,000. Unlike nitrogen removal, phosphorous removal was mostly dependent on adsorption due to dolochar and fine soil particles produced by earthworms. Maximum removal efficiencies of 61% and 44% were observed for PO43-P and total phosphates (TP), respectively. The results obtained indicate that DO plays a crucial role in vermifiltration and that EWD and OLR play pivotal roles in maintaining DO in the effluents obtained from vermifiltration.

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Acknowledgments

The authors wish to thank the Department of Civil Engineering, School of Infrastructure, Indian Institute of Technology Bhubaneswar, India, for providing facilities for carrying out research work in the related area.

References

Aira, M., and J. Domínguez. 2009. “Microbial and nutrient stabilization of two animal manures after the transit through the gut of the earthworm Eisenia fetida.” J. Hazard. Mater. 161 (2–3): 1234–1238. https://doi.org/10.1016/j.jhazmat.2008.04.073.
Akhavan, M., P. T. Imhoff, A. S. Andres, and S. Finstrle. 2013. “Model evaluation of denitrification under rapid infiltration basin systems.” J. Contam. Hydrol. 152: 18–34. https://doi.org/10.1016/j.jconhyd.2013.05.007.
APHA, AWWA, and WPCF (American Public Health Association, American Water Works Association, and Water Pollution Control Federation). 2005. Standard methods for the examination of water and wastewater. 21st ed. Washington, DC: APHA, AWWA, and WPCE.
Arias, C. A., M. Del Bubba, and H. Brix. 2001. “Phosphorus removal by sands for use as media in subsurface flow constructed reed beds.” Water Res. 35 (5): 1159–1168. https://doi.org/10.1016/S0043-1354(00)00368-7.
Arora, S., and A. A. Kazmi. 2015. “The effect of seasonal temperature on pathogen removal efficacy of vermifilter for wastewater treatment.” Water Res. 74: 88–99. https://doi.org/10.1016/j.watres.2015.02.001.
Arora, S., A. Rajpal, R. Bhargava, V. Pruthi, A. Bhatia, and A. A. Kazmi. 2014a. “Antibacterial and enzymatic activity of microbial community during wastewater treatment by pilot scale vermifiltration system.” Bioresour. Technol. 166: 132–141. https://doi.org/10.1016/j.biortech.2014.05.041.
Arora, S., A. Rajpal, and A. A. Kazmi. 2016. “Antimicrobial activity of bacterial community for removal of pathogens during vermifiltration.” J. Environ. Eng. 142 (5): 04016012. https://doi.org/10.1061/(ASCE)EE.1943-7870.0001080.
Arora, S., A. Rajpal, T. Kumar, R. Bhargava, and A. A. Kazmi. 2014b. “Pathogen removal during wastewater treatment by vermifiltration.” Environ. Technol. 35 (19): 2493–2499. https://doi.org/10.1080/09593330.2014.911358.
Binet, F., L. Fayolle, and M. Pussard. 1998. “Significance of earthworms in stimulating soil microbial activity.” Biol. Fertil. Soils 27 (1): 79–84. https://doi.org/10.1007/s003740050403.
Brown, G. G. 1995. “How do earthworms affect microbial and faunal community diversity?” Plant Soil 170 (1): 209–231. https://doi.org/10.1007/BF02183068.
Dai, H., X. Yang, T. Dong, Y. Ke, and T. Wang. 2010. “Engineering application of MBR process to the treatment of beer brewing wastewater.” Mod. Appl. Sci. 4 (9): 103–109. https://doi.org/10.5539/mas.v4n9p103.
Del Bubba, M., C. A. Arias, and H. Brix. 2003. “Phosphorus adsorption maximum of sands for use as media in subsurface flow constructed reed beds as measured by the Langmuir isotherm.” Water Res. 37 (14): 3390–3400. https://doi.org/10.1016/S0043-1354(03)00231-8.
Dhadse, S., S. Satyanarayan, P. R. Chaudhari, and S. R. Wate. 2010. “Vermifilters: A tool for aerobic biological treatment of herbal pharmaceutical wastewater.” Water Sci. Technol. 61 (9): 2375–2380. https://doi.org/10.2166/wst.2010.523.
Fang, C. X., Z. Zheng, X. Z. Luo, and F. H. Guo. 2010. “Effect of hydraulic load on domestic wastewater treatment and removal mechanism of phosphorus in earthworm ecofilter.” Fresenius Environ. Bull. 19: 1099–1108.
Feng, Y., X. Wang, B. E. Logan, and H. Lee. 2008. “Brewery wastewater treatment using aircathode microbial fuel cells.” Appl. Microbiol. Biotechnol. 78 (5): 873–880. https://doi.org/10.1007/s00253-008-1360-2.
Gunadi, B., C. Blount, and C. A. Edwards. 2002. “The growth and fecundity of Eisenia fetida (Savigny) in cattle solids pre-composted for different periods.” Pedobiologia 46 (1): 15–23. https://doi.org/10.1078/0031-4056-00109.
Holenda, B., E. Domokos, A. R. Redey, and J. Fazakasb. 2008. “Dissolved oxygen control of the activated sludge wastewater treatment process using model predictive control.” Comput. Chem. Eng. 32 (6): 1270–1278. https://doi.org/10.1016/j.compchemeng.2007.06.008.
Jiang, L., et al. 2016. “The use of microbial-earthworm ecofilters for wastewater treatment with special attention to influencing factors in performance: A review.” Bioresour. Technol. 200: 999–1007. https://doi.org/10.1016/j.biortech.2015.11.011.
Krishnasamy, K., J. Nair, and R. J. Hughes. 2013. “Vermifiltration systems for liquid waste management: A review.” Int. J. Environ. Waste Manage. 12 (4): 382–396. https://doi.org/10.1504/IJEWM.2013.056908.
Kumar, T., R. Bhargava, K. S. H. Prasad, and V. Pruthi. 2015. “Evaluation of vermifiltration process using natural ingredients for effective wastewater treatment.” Ecol. Eng. 75: 370–377. https://doi.org/10.1016/j.ecoleng.2014.11.044.
Kumar, T., A. Rajpal, S. Arora, R. Bhargava, K. S. H. Prasad, and A. A. Kazmi. 2016. “A comparative study on vermifiltration using epigeic earthworm Eisenia fetida and Eudrilus eugeniae.” Desalin. Water Treat. 57 (14): 6347–6354. https://doi.org/10.1080/19443994.2015.1010230.
Kumar, T., A. Rajpal, R. Bhargava, and K. S. H. Prasad. 2015. “Performance evaluation of vermifilter at different hydraulic loading rate using riverbed material.” Ecol. Eng. 62: 77–82. https://doi.org/10.1016/j.ecoleng.2013.10.028.
Li, H. Z., S. Wang, J. F. Ye, Z. X. Xu, and W. Jin. 2011. “A practical method for the restoration of clogged rural vertical subsurface flow constructed wetlands for domestic wastewater treatment using earthworm.” Water Sci. Technol. 63 (2): 283–290. https://doi.org/10.2166/wst.2011.051.
Liu, J., Z. Lu, J. Zhang, M. Xing, and J. Yang. 2013. “Phylogenetic characterization of microbial communities in a full-scale vermifilter treating rural domestic sewage.” Ecol. Eng. 61: 100–109. https://doi.org/10.1016/j.ecoleng.2013.09.015.
Luth, R. P., P. Germain, M. Lecomte, B. Landrain, Y. S. Li, and D. Cluzeau. 2011. “Earthworm effects on gaseous emissions during vermifiltration of pig fresh slurry.” Bioresour. Technol. 102 (4): 3679–3686. https://doi.org/10.1016/j.biortech.2010.11.027.
Manyuchi, M. M., C. Mbohwa, and E. Muzenda. 2018. “Biological treatment of distillery wastewater by application of the vermifiltration technology.” S. Afr. J. Chem. Eng. 25: 74–78. https://doi.org/10.1016/j.sajce.2017.12.002.
Mata, T. M., A. C. Melo, M. Simoes, and N. S. Caetano. 2012. “Parametric study of a brewery effluent treatment by microalgae.” Bioresour. Technol. 107: 151–158. https://doi.org/10.1016/j.biortech.2011.12.109.
Nie, E., D. Wang, M. Yang, X. Luo, C. Fang, X. Yang, D. Su, L. Zhou, and Z. Zheng. 2015. “Tower bio-vermifilter system for rural wastewater treatment: Bench-scale, pilot-scale, and engineering applications.” Int. J. Environ. Sci. Technol. 12 (3): 1053–1064. https://doi.org/10.1007/s13762-013-0479-6.
Platzer, C., and K. Mauch. 1997. “Soil clogging in vertical flow reed beds mechanisms, parameters, consequences and…….solutions?” Water Sci. Technol. 35 (5): 175–181. https://doi.org/10.2166/wst.1997.0191.
Rout, P. R., P. Bhunia, and R. R. Dash. 2017. “Response surface optimization of phosphate removal from aqueous solution using a natural adsorbent.” In Trends in Asian water environmental science and technology, 93–104. Cham, Switzerland: Springer.
Rout, P. R., R. R. Dash, and P. Bhunia. 2015. “Effective utilization of a sponge iron industry by-product for phosphate removal from aqueous solution: A statistical and kinetic modelling approach.” J. Taiwan Inst. Chem. Eng. 46: 98–108. https://doi.org/10.1016/j.jtice.2014.09.006.
Saeed, T., and G. Sun. 2012. “A review on nitrogen and organics removal mechanisms in subsurface flow constructed wetlands: Dependency on environmental parameters, operating conditions and supporting media.” J. Environ. Manage. 112: 429–448. https://doi.org/10.1016/j.jenvman.2012.08.011.
Samal, K., R. R. Dash, and P. Bhunia. 2017. “Treatment of wastewater by vermifiltration integrated with macrophyte filter: A review.” J. Environ. Chem. Eng. 5 (3): 2274–2289. https://doi.org/10.1016/j.jece.2017.04.026.
Sen, B., and T. S. Chandra. 2009. “Do earthworms affect dynamics of functional response and genetic structure of microbial community in a lab-scale composting system?” Bioresour. Technol. 100 (2): 804–811. https://doi.org/10.1016/j.biortech.2008.07.047.
Simate, G. S., J. Cluett, S. E. Lyuke, E. T. Musapatika, S. Ndlovu, L. F. Walubita, and A. E. Alvarez. 2011. “The treatment of brewery wastewater for reuse: State of the art.” Desalination 273 (2–3): 235–247. https://doi.org/10.1016/j.desal.2011.02.035.
Singh, R., P. Bhunia, and R. R. Dash. 2017. “A mechanistic review on vermifiltration of wastewater: Design, operation and performance.” J. Environ. Manage. 197: 656–672. https://doi.org/10.1016/j.jenvman.2017.04.042.
Singh, R., P. Bhunia, and R. R. Dash. 2018a. “COD removal index—A mechanistic tool for predicting organics removal performance of vermifilters.” Sci. Tot. Environ. 643: 1652–1659. https://doi.org/10.1016/j.scitotenv.2018.07.272.
Singh, R., P. Bhunia, and R. R. Dash. 2018b. “Understanding intricacies of clogging and its alleviation by introducing earthworms in soil biofilters.” Sci. Tot. Environ. 633: 145–156. https://doi.org/10.1016/j.scitotenv.2018.03.156.
Singh, R., P. Bhunia, and R. R. Dash. 2019. “Optimization of organics removal and understanding the impact of HRT on treatment of brewery wastewater using vermifilter.” Sci. Tot. Environ. 651: 1283–1293. https://doi.org/10.1016/j.scitotenv.2018.09.307.
Sinha, R. K., G. Bharambe, and U. Chaudhari. 2008a. “Sewage treatment by vermifiltration with synchronous treatment of sludge by earthworms: A low cost sustainable technology over conventional systems with potential for decentralization.” Environmentalist 28 (4): 409–420. https://doi.org/10.1007/s10669-008-9162-8.
Sinha, R. K., V. Chandran, B. K. Soni, U. Patel, and A. Ghosh. 2012. “Earthworms: Nature’s chemical managers and detoxifying agents in the environment: An innovative study on treatment of toxic wastewaters from the petroleum industry by vermifiltration technology.” Environmentalist 32 (4): 445–452. https://doi.org/10.1007/s10669-012-9409-2.
Sinha, R. K., J. Nair, G. Bharambe, S. Patil, and P. Bapat. 2008b. “Vermiculture revolution: A low-cost and sustainable technology for management of municipal and industrial organic wastes (solid and liquid) by earthworms with significantly low greenhouse gas emissions.” In Progress in waste management research, edited by J. I. Daven and R. N. Klein, 159–227. New York: Nova Science Publishers.
Tam, K., C. Yang, M. R. Matsumoto, D. E. Crowley, and J. D. Sheppard. 2005. “Comparison of PCR-DGGE and selective plating methods for monitoring the dynamics of a mixed culture population in synthetic brewery wastewater.” Biotechnol. Prog. 21 (3): 712–719. https://doi.org/10.1021/bp050107w.
Tanner, C. C. 2001. “Plants as ecosystem engineers in subsurface-flow treatment wetlands.” Water Sci. Technol. 44 (11–12): 9–17. https://doi.org/10.2166/wst.2001.0804.
Taylor, M., W. P. Clarke, and P. F. Greenfield. 2003. “The treatment of domestic wastewater using small-scale vermicompost filter beds.” Ecol. Eng. 21 (2–3): 197–203. https://doi.org/10.1016/j.ecoleng.2003.12.003.
Tchobanoglous, G., F. L. Burton, and H. D. Stensel. 2003. Wastewater engineering. treatment and reuse. 4th ed. New York: McGraw-Hill.
Tomar, P., and S. Suthar. 2011. “Urban wastewater treatment using vermi-biofiltration system.” Desalination 282: 95–103. https://doi.org/10.1016/j.desal.2011.09.007.
Vyamazal, J. 2005. “Horizontal sub-surface flow and hybrid constructed wetlands systems for wastewater treatment.” Ecol. Eng. 25 (5): 478–490. https://doi.org/10.1016/j.ecoleng.2005.07.010.
Wang, D. B., et al. 2010. “Effects of earthworms on surface clogging characteristics of intermittent sand filters.” Water Sci. Technol. 61 (11): 2881–2888. https://doi.org/10.2166/wst.2010.180.
Wang, L., F. Guo, Z. Zheng, X. Luo, and J. Zhang. 2011. “Enhancement of rural domestic sewage treatment performance, and assessment of microbial community diversity and structure using tower vermifiltration.” Bioresour. Technol. 102 (20): 9462–9470. https://doi.org/10.1016/j.biortech.2011.07.085.

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Go to Journal of Hazardous, Toxic, and Radioactive Waste
Journal of Hazardous, Toxic, and Radioactive Waste
Volume 23Issue 2April 2019

History

Received: Jun 17, 2018
Accepted: Sep 6, 2018
Published online: Jan 23, 2019
Published in print: Apr 1, 2019
Discussion open until: Jun 23, 2019

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Rajneesh Singh [email protected]
Research Scholar, School of Infrastructure, Indian Institute of Technology Bhubaneswar, Bhubaneswar, Odisha 752050, India. Email: [email protected]
Puspendu Bhunia [email protected]
Associate Professor, School of Infrastructure, Indian Institute of Technology Bhubaneswar, Bhubaneswar, Odisha 752050, India (corresponding author). Email: [email protected]; [email protected]
Rajesh R. Dash [email protected]
Associate Professor, School of Infrastructure, Indian Institute of Technology Bhubaneswar, Bhubaneswar, Odisha 752050, India. Email: [email protected]

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