Technical Papers
Mar 22, 2022

Enhancing Soil Strength at Targeted Calcite Content via Optimizing Chemical Application Parameters Using Taguchi Method for Biocementation

Publication: Journal of Geotechnical and Geoenvironmental Engineering
Volume 148, Issue 6

Abstract

Soil strength improvement employing microbial induced calcite precipitation (MICP) involves bio-geo-chemical interaction among bacteria (here, Sporosarcina pasteurii), sand, and cementing solution. A critical review of literature opened the gap in exploring the strength gain for a given amount of CaCO3 precipitation, investigating the influence of chemical concentration and its application methodology. Thereby, the current study aims to independently maximize the calcite precipitation efficiency and unconfined compressive strength while targeting the calcite deposition of 10%–12%. In the present study, the Taguchi method of experimental design followed by analysis of variance and analysis of mean was adopted to optimize the outcome parameters. Results depicted the extreme variation in obtained strength in a small range of calcite deposition. Further, the microscopic investigation employing micrographs and elemental mapping reveals a higher impact of CaCO3 depositional characteristics than its amount on strength gain. The current study envisages the importance of chemical application parameters to improve efficiency and economy while reducing soil contamination due to non-reacting chemicals.

Get full access to this article

View all available purchase options and get full access to this article.

Data Availability Statement

Some or all data, models, or codes that support the findings of this study are available from the corresponding author upon reasonable request.

Acknowledgments

The authors would like to express their gratitude to the Bio-X center and Advanced Material Research Centre (AMRC) of IIT Mandi, for providing microbiological and other testing facilities.

References

Al Qabany, A., and K. Soga. 2013. “Effect of chemical treatment used in MICP on engineering properties of cemented soils.” In Proc., Bio- and Chemo-Mechanical Processes in Geotechnical Engineering—Geotechnique Symp. in Print 2013, 107–115. London: ICE Publishing.
Al Qabany, A., K. Soga, and C. Santamarina. 2012. “Factors affecting efficiency of microbially induced calcite precipitation.” J. Geotech. Geoenviron. Eng. 138 (8): 992–1001. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000666.
ASTM. 2006. Standard test methods for maximum index density and unit weight of soils using a vibratory table. ASTM D4253-00. West Conshohocken, PA: ASTM.
ASTM. 2016. Standard test method for unconfined compressive strength of cohesive soil. ASTM D2166. West Conshohocken, PA: ASTM.
Bachmeier, K. L., A. E. Williams, J. R. Warmington, and S. S. Bang. 2002. “Urease activity in microbiologically-induced calcite precipitation.” J. Biotechnol. 93 (2): 171–181. https://doi.org/10.1016/S0168-1656(01)00393-5.
Benini, S., C. Gessa, and S. Ciurli. 1996. “Bacillus pasteurii urease: A heteropolymeric enzyme with a binuclear nickel active site.” Soil Biol. Biochem. 28 (6): 819–821. https://doi.org/10.1016/0038-0717(96)00017-X.
Cheng, L., and R. Cord-Ruwisch. 2012. “In situ soil cementation with ureolytic bacteria by surface percolation.” Ecol. Eng. 42 (May): 64–72. https://doi.org/10.1016/j.ecoleng.2012.01.013.
Cheng, L., R. Cord-Ruwisch, and M. A. Shahin. 2013. “Cementation of sand soil by microbially induced calcite precipitation at various degrees of saturation.” Can. Geotech. J. 50 (1): 81–90. https://doi.org/10.1139/cgj-2012-0023.
Cheng, L., M. A. Shahin, and R. Cord-Ruwisch. 2014. “Bio-cementation of sandy soil using microbially induced carbonate precipitation for marine environments.” Géotechnique 64 (12): 1010–1013. https://doi.org/10.1680/geot.14.T.025.
Cheng, L., M. A. Shahin, and D. Mujah. 2017. “Influence of key environmental conditions on microbially induced cementation for soil stabilization.” J. Geotech. Geoenviron. Eng. 143 (1): 04016083. https://doi.org/10.1061/(ASCE)GT.1943-5606.0001586.
Choi, S.-G., S. Wu, and J. Chu. 2016. “Biocementation for sand using an eggshell as calcium source.” J. Geotech. Geoenviron. Eng. 142 (10): 06016010. https://doi.org/10.1061/(ASCE)GT.1943-5606.0001534.
Chu, J., V. Ivanov, M. Naeimi, V. Stabnikov, and H. L. Liu. 2014. “Optimization of calcium-based bioclogging and biocementation of sand.” Acta Geotech. 9 (2): 277–285. https://doi.org/10.1007/s11440-013-0278-8.
Chu, J., V. Stabnikov, and V. Ivanov. 2012. “Microbially induced calcium carbonate precipitation on surface or in the bulk of soil.” Geomicrobiol. J. 29 (6): 544–549. https://doi.org/10.1080/01490451.2011.592929.
DeJong, J. T., M. B. Fritzges, and K. Nüsslein. 2006. “Microbially induced cementation to control sand response to undrained shear.” J. Geotech. Geoenviron. Eng. 132 (11): 1381–1392. https://doi.org/10.1061/(ASCE)1090-0241(2006)132:11(1381).
DeJong, J. T., B. M. Mortensen, B. C. Martinez, and D. C. Nelson. 2010. “Bio-mediated soil improvement.” Ecol. Eng. 36 (2): 197–210. https://doi.org/10.1016/j.ecoleng.2008.12.029.
Dhami, N. K., M. S. Reddy, and A. Mukherjee. 2016. “Significant indicators for biomineralisation in sand of varying grain sizes.” Constr. Build. Mater. 104 (Feb): 198–207. https://doi.org/10.1016/j.conbuildmat.2015.12.023.
Falini, G., S. Albeck, S. Weiner, and L. Addadi. 1996. “Control of aragonite or calcite polymorphism by mollusk shell macromolecules.” Science 271 (5245): 67–69. https://doi.org/10.1126/science.271.5245.67.
Foppen, J. W. A., and J. F. Schijven. 2006. “Evaluation of data from the literature on the transport and survival of Escherichia coli and thermotolerant coliforms in aquifers under saturated conditions.” Water Res. 40 (3): 401–426. https://doi.org/10.1016/j.watres.2005.11.018.
Ismail, M. A., H. A. Joer, M. F. Randolph, and A. Meritt. 2002a. “Cementation of porous materials using calcite.” Géotechnique 52 (5): 313–324. https://doi.org/10.1680/geot.2002.52.5.313.
Ismail, M. A., H. A. Joer, W. H. Sim, and M. F. Randolph. 2002b. “Effect of cement type on shear behavior of cemented calcareous soil.” J. Geotech. Geoenviron. Eng. 128 (6): 520–529. https://doi.org/10.1061/(ASCE)1090-0241(2002)128:6(520).
Mahawish, A., A. Bouazza, and W. P. Gates. 2018. “Effect of particle size distribution on the bio-cementation of coarse aggregates.” Acta Geotech. 13 (4): 1019–1025. https://doi.org/10.1007/s11440-017-0604-7.
Mahawish, A., A. Bouazza, and W. P. Gates. 2019. “Factors affecting the bio-cementing process of coarse sand.” Proc. Inst. Civ. Eng. Ground Improv. 172 (1): 25–36.
Martinez, B. C., J. T. Dejong, T. R. Ginn, B. M. Montoya, T. H. Barkouki, C. Hunt, B. Tanyu, and D. Major. 2013. “Experimental optimization of microbial-induced carbonate precipitation for soil improvement.” J. Geotech. Geoenviron. Eng. 139 (4): 587–598. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000787.
Menten, T. 1991. “Quality engineering using robust design.” Technometrics 33 (2): 235–236.
Mirmohammad Sadeghi, M., A. R. Modarresnia, and F. Shafiei. 2015. “Parameters effects evaluation of microbial strengthening of sandy soils in mixing experiments using Taguchi methodology.” Geomicrobiol. J. 32 (5): 453–465. https://doi.org/10.1080/01490451.2014.958206.
Mori, D., P. Jyoti, T. Thakur, S. K. Masakapalli, and K. V. Uday. 2020. “Influence of cementing solution concentration on calcite precipitation pattern in biocementation.” In Advances in computer methods and geomechanics, 737–746. Singapore: Springer.
Mori, D., and K. V. Uday. 2021. “A review on qualitative interaction among the parameters affecting ureolytic microbial-induced calcite precipitation.” Environ. Earth Sci. 80 (8): 329. https://doi.org/10.1007/s12665-021-09613-7.
Rowshanbakht, K., M. Khamehchiyan, R. H. Sajedi, and M. R. Nikudel. 2016. “Effect of injected bacterial suspension volume and relative density on carbonate precipitation resulting from microbial treatment.” Ecol. Eng. 89 (Apr): 49–55. https://doi.org/10.1016/j.ecoleng.2016.01.010.
Shahrokhi-Shahraki, R., S. M. A. Zomorodian, A. Niazi, and B. C. O’Kelly. 2015. “Improving sand with microbial-induced carbonate precipitation.” Proc. Inst. Civ. Eng. Ground Improv. 168 (3): 217–230. https://doi.org/10.1680/grim.14.00001.
Sharma, M., N. Satyam, and K. R. Reddy. 2021. “Rock-like behavior of biocemented sand treated under non-sterile environment and various treatment conditions.” J. Rock Mech. Geotech. Eng. 13 (3): 705–716. https://doi.org/10.1016/j.jrmge.2020.11.006.
Shashank, B. S., J. M. Minto, D. N. Singh, G. El Mountassir, and C. W. Knapp. 2018. “Guidance for investigating calcite precipitation by urea hydrolysis for geomaterials.” J. Test. Eval. 46 (4): 20170122. https://doi.org/10.1520/JTE20170122.
Soon, N. W., L. M. Lee, T. C. Khun, and H. S. Ling. 2014. “Factors affecting improvement in engineering properties of residual soil through microbial-induced calcite precipitation.” J. Geotech. Geoenviron. Eng. 140 (5): 04014006. https://doi.org/10.1061/(ASCE)GT.1943-5606.0001089.
Sotoudehfar, A. R., M. Mirmohammad Sadeghi, E. Mokhtari, and F. Shafiei. 2016. “Assessment of the parameters influencing microbial calcite precipitation in injection experiments using Taguchi methodology.” Geomicrobiol. J. 33 (2): 163–172. https://doi.org/10.1080/01490451.2015.1025316.
Stocks-Fischer, S., J. K. Galinat, and S. S. Bang. 1999. “Microbiological precipitation of CaCO3.” Soil Biol. Biochem. 31 (11): 1563–1571. https://doi.org/10.1016/S0038-0717(99)00082-6.
Terzis, D., and L. Laloui. 2019. “A decade of progress and turning points in the understanding of bio-improved soils: A review.” Geomech. Energy Environ. 19 (Sep): 100116. https://doi.org/10.1016/j.gete.2019.03.001.
Tobler, D. J., E. Maclachlan, and V. R. Phoenix. 2012. “Microbially mediated plugging of porous media and the impact of differing injection strategies.” Ecol. Eng. 42 (May): 270–278. https://doi.org/10.1016/j.ecoleng.2012.02.027.
van Paassen, L. A., R. Ghose, T. J. M. van der Linden, W. R. L. van der Star, and M. C. M. van Loosdrecht. 2010. “Quantifying biomediated ground improvement by ureolysis: Large-scale biogrout experiment.” J. Geotech. Geoenviron. Eng. 136 (12): 1721–1728. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000382.
Wang, Y., K. Soga, J. T. Dejong, and A. J. Kabla. 2019a. “A microfluidic chip and its use in characterising the particle-scale behaviour of microbial-induced calcium carbonate precipitation (MICP).” Géotechnique 69 (12): 1086–1094. https://doi.org/10.1680/jgeot.18.P.031.
Wang, Y., K. Soga, J. T. Dejong, and A. J. Kabla. 2019b. “Microscale visualization of microbial-induced calcium carbonate precipitation processes.” J. Geotech. Geoenviron. Eng. 145 (9): 04019045. https://doi.org/10.1061/(ASCE)GT.1943-5606.0002079.
Wang, Z., N. Zhang, G. Cai, Y. Jin, N. Ding, and D. Shen. 2017. “Review of ground improvement using microbial induced carbonate precipitation (MICP).” Mar. Georesour. Geotechnol. 35 (8): 1135–1146. https://doi.org/10.1080/1064119X.2017.1297877.
Whiffin, V. S., L. A. van Paassen, and M. P. Harkes. 2007. “Microbial carbonate precipitation as a soil improvement technique.” Geomicrobiol. J. 24 (5): 417–423. https://doi.org/10.1080/01490450701436505.
Yasuhara, H., D. Neupane, K. Hayashi, and M. Okamura. 2012. “Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation.” Soils Found. 52 (3): 539–549. https://doi.org/10.1016/j.sandf.2012.05.011.
Zhao, Q., L. Li, C. Li, M. Li, F. Amini, and H. Zhang. 2014. “Factors affecting improvement of engineering properties of MICP-treated soil catalyzed by bacteria and urease.” J. Mater. Civ. Eng. 26 (12): 04014094. https://doi.org/10.1061/(ASCE)MT.1943-5533.0001013.

Information & Authors

Information

Published In

Go to Journal of Geotechnical and Geoenvironmental Engineering
Journal of Geotechnical and Geoenvironmental Engineering
Volume 148Issue 6June 2022

History

Received: Oct 23, 2020
Accepted: Feb 1, 2022
Published online: Mar 22, 2022
Published in print: Jun 1, 2022
Discussion open until: Aug 22, 2022

Permissions

Request permissions for this article.

Authors

Affiliations

Deepak Mori [email protected]
M.S. Research Scholar, School of Engineering, Indian Institute of Technology Mandi, Mandi, Himachal Pradesh 175005, India. Email: [email protected]
Assistant Professor, School of Engineering, Indian Institute of Technology Mandi, Mandi, Himachal Pradesh 175005, India (corresponding author). ORCID: https://orcid.org/0000-0002-9579-5496. Email: [email protected]

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share