Abstract

Microbially induced calcite precipitation (MICP) is a ground improvement technique that uses ureolytic bacteria to biocement soils by precipitating calcium carbonate on soil contacts and surfaces. In this study, five 3.7-m long horizontal columns, each containing 0.15  m3 of natural sand, were treated to address current knowledge gaps regarding the application of MICP at field-scale including investigating treatment extent, improvement of spatial uniformity, stimulation strategies, and posttreatment removal of ammonium by-products. Two stimulated columns containing poorly graded alluvial sand were treated to achieve high and low ureolytic rates to investigate differences in treatment uniformity and extent. An additional column with the same sand was augmented with Sporosarcina pasteurii targeting a high ureolytic rate similar to the high rate stimulated column. Two additional stimulated columns, containing poorly graded alluvial and marine sands, were treated to achieve a low ureolytic rate to evaluate the effect of soil mineralogy. Treatments were first applied to establish either indigenous or augmented microorganisms, after which all columns received nine cementation treatments. Following cementation, produced ammonium by-products were removed using a rinse solution injection and soil samples were obtained and analyzed for calcite content. During all treatments, solution urea and ammonium concentrations as well as soil hydraulic conductivities and shear wave velocities were monitored. In all columns, insignificant changes in permeability were observed during treatments despite Vs increases up to 1,325  m/s. Results suggest that improvements in the spatial uniformity and extent of biocementation can be achieved through the use of lower ureolytic rates that minimize reactions during injections.

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Data Availability Statement

Some or all data, models, or code generated or used during the study are available from the corresponding author by request.

Acknowledgments

The presented study involves work supported by the Engineering Research Center Program of the National Science Foundation under NSF Cooperative Agreement No. EEC-1449501. Any opinions, findings, and conclusions or recommendations expressed in this manuscript are those of the authors and do not necessarily reflect the views of the National Science Foundation. The authors would like to thank Daret Kehlet, Bill Sluis, Victor Jones, Lin Huang, Tamar Baumer, Matthew Burrall, Maya El Kortbawi, and Emmanuel Vasquez for their help with fabricating the columns and soil sampling.

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Go to Journal of Geotechnical and Geoenvironmental Engineering
Journal of Geotechnical and Geoenvironmental Engineering
Volume 146Issue 11November 2020

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Received: Nov 4, 2019
Accepted: Jun 18, 2020
Published online: Sep 10, 2020
Published in print: Nov 1, 2020
Discussion open until: Feb 10, 2021

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Alexandra C. M. San Pablo, S.M.ASCE [email protected]
Ph.D. Student, Dept. of Civil and Environmental Engineering, Univ. of California, Davis, Davis, CA 95616. Email: [email protected]
Minyong Lee, S.M.ASCE [email protected]
Ph.D. Student, Dept. of Civil and Environmental Engineering, Univ. of Washington, 201 More Hall, P.O. Box 352700, Seattle, WA 98195. Email: [email protected]
Charles M. R. Graddy [email protected]
Ph.D. Student, Dept. of Microbiology and Molecular Genetics, Univ. of California, Davis, Davis, CA 95616. Email: [email protected]
Colin M. Kolbus [email protected]
Undergraduate Student, Dept. of Civil and Environmental Engineering, Univ. of Washington, 201 More Hall, P.O. Box 352700, Seattle, WA 98195. Email: [email protected]
Mahanoor Khan [email protected]
Undergraduate Student, Dept. of Microbiology and Molecular Genetics, Univ. of California, Davis, Davis, CA 95616. Email: [email protected]
Atefeh Zamani [email protected]
Postdoctoral Researcher, Dept. of Civil and Environmental Engineering, Univ. of California, Davis, Davis, CA 95616. Email: [email protected]
Nina Martin [email protected]
High School Student, Davis High School, Davis, CA 95616. Email: [email protected]
Catalina Acuff [email protected]
High School Student, Acalanes High School, Lafayette, CA 94549. Email: [email protected]
Professor, Dept. of Civil and Environmental Engineering, Univ. of California, Davis, Davis, CA 95616 (corresponding author). ORCID: https://orcid.org/0000-0002-9809-955X. Email: [email protected]
Assistant Professor, Dept. of Civil and Environmental Engineering, Univ. of Washington, Seattle, WA 98105. ORCID: https://orcid.org/0000-0002-4464-5447. Email: [email protected]
Douglas C. Nelson [email protected]
Professor Emeritus, Dept. of Microbiology and Molecular Genetics, Univ. of California, Davis, Davis, CA 95616. Email: [email protected]

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