Investigation of Two-Stage Membrane Bioreactor Potential of Activated Sludge and Microalgae in Municipal Wastewater Treatment
Publication: Journal of Environmental Engineering
Volume 148, Issue 12
Abstract
The combination of activated sludge and microalgae in a membrane bioreactor (MBR) is a new promising solution in municipal wastewater treatment to remove nitrate () and phosphate (). Furthermore, municipal wastewater contains a considerable amount of and , which is suitable for microalgae growth. It can be concluded from the literature that there is a direct relationship between microalgae biomass production and nutrient removal. Thus, the light cycle and the initial microalgae concentration (as two main factors for microalgae growth) can be optimized. In this study, the biomass productivity in three light-dark (L-D) cycles (h) (24-0, 16-8, and 12-12 h) and initial concentrations (0.5, 1, and ) were measured. The L-D cycle of 24-0 and the initial concentration of was used as the chosen parameter in the membrane photobioreactor (MPBR) containing microalgae. The initial concentration of nitrate, phosphate, chemical oxygen demand (COD), and total dissolved solids (TDS) was investigated. The investigation was performed first on the MBR containing activated sludge and then on the treated wastewater via MBR to MPBR containing microalgae (MBR-MPBR). The duration of the experiment was 133 days. During this period, the removal amount of COD, nitrate, and phosphate was reported at 92.35%, 47.55%, and 36.46% in the MBR, respectively. In MBR-MPBR, the average of nitrate and phosphate removal increased to 93.88% and 88.44%, respectively. However, TDS did not show any notable alterations in both operating systems. According to the findings, the second stage of the treatment process, MBR-MPBR outperformed the MBR system in terms of and removal.
Practical Applications
A membrane bioreactor is a combination of the membrane with microorganisms used in the wastewater treatment process. Due to their simple structure, small size, and high efficiency in removing organic and inorganic substances, membrane bioreactors have been considered for industrial and urban wastewater treatment. Operational parameters in the design of these systems have a significant impact on nutrient removal efficiency. Furthermore, based on the high efficiency of microalgae in removing nutrients, its combination with activated sludge increases the efficiency of the bioreactor. In this work, some operating parameters such as the light-dark cycle and initial concentration of microalgae were optimized in the membrane bioreactor. Then, two various systems, namely a membrane bioreactor (containing activated sludge) and a two-stage membrane bioreactor (containing activated sludge and microalgae), were compared. In this regard, the total dissolved solids, chemical oxygen demand, nitrate, and phosphate removal were investigated. All these values were recorded under optimal conditions of the light-dark cycle and initial concentration of microalgae in two different systems.
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Data Availability Statement
All data, models, and code generated or used during the study appear in the published article.
Acknowledgments
The authors would like to thank Mr. Soroush Azizi and Mr. Sina Zalbegi for their untiring assistance throughout this project.
References
Abou-Shanab, R. A., M. K. Ji, H. C. Kim, K. J. Paeng, and B. H. Jeon. 2013. “Microalgal species growing on piggery wastewater as a valuable candidate for nutrient removal and biodiesel production.” J. Environ. Manage. 115 (Jan): 257–264. https://doi.org/10.1016/j.jenvman.2012.11.022.
Ali, I., O. M. Alharbi, Z. A. Alothman, and A. Y. Badjah. 2018. “Kinetics, thermodynamics, and modeling of amido black dye photodegradation in water using Co/TiO2 nanoparticles.” Photochem. Photobiol. 94 (5): 935–941. https://doi.org/10.1111/php.12937.
Ali, I., and C. K. Jain. 1998. “Groundwater contamination and health hazards by some of the most commonly used pesticides.” Curr. Sci. 75 (10): 1011–1014.
Altmann, J., A. Sperlich, and M. Jekel. 2015. “Integrating organic micropollutant removal into tertiary filtration: Combining PAC adsorption with advanced phosphorus removal.” Water Res. 84 (Feb): 58–65. https://doi.org/10.1016/j.watres.2015.07.023.
Amini, M., Z. A. Khoei, and E. Erfanifar. 2019. “Nitrate (NO3−) and phosphate (PO43−) removal from aqueous solutions by microalgae Dunaliella salina.” Biocatal. Agric. Biotechnol. 19 (10): 101097. https://doi.org/10.1016/j.bcab.2019.101097.
APHA (American Public Health Association). 2005. Standard methods for the examination of water and wastewater. Washington, DC: APHA.
Arévalo, J., L. M. Ruiz, J. Pérez, and M. A. Gómez. 2014. “Effect of temperature on membrane bioreactor performance working with high hydraulic and sludge retention time.” Biochem. Eng. J. 88 (Mar): 42–49. https://doi.org/10.1016/j.bej.2014.03.006.
Arias, D. M., M. Solé-Bundó, M. Garfí, I. Ferrer, J. García, and E. Uggetti. 2018. “Integrating microalgae tertiary treatment into activated sludge systems for energy and nutrients recovery from wastewater.” Bioresour. Technol. 247 (May): 513–519. https://doi.org/10.1016/j.biortech.2017.09.123.
Azizi, S., B. Bayat, H. Tayebati, A. Hashemi, and F. Pajoum Shariati. 2021a. “Nitrate and phosphate removal from treated wastewater by Chlorella vulgaris under various light regimes within membrane flat plate photobioreactor.” Environ. Prog. Sustainable Energy 40 (2): e13519. https://doi.org/10.1002/ep.13519.
Azizi, S., A. Hashemi, F. Pajoum Shariati, B. Bonakdarpour, and M. Safamirzaei. 2021b. “Fouling identification in reciprocal membrane photobioreactor (RMPBR) containing Chlorella vulgaris species: Hydraulic resistances assessment.” J. Chem. Technol. Biotechnol. 96 (2): 404–411. https://doi.org/10.1002/jctb.6552.
Azizi, S., A. Hashemi, F. Pajoum Shariati, H. Tayebati, A. Keramati, B. Bonakdarpour, and M. M. A. Shirazi. 2021c. “Effect of different light-dark cycles on the membrane fouling, EPS and SMP production in a novel reciprocal membrane photobioreactor (RMPBR) by C. vulgaris species.” J. Water Process Eng. 43 (21): 102256. https://doi.org/10.1016/j.jwpe.2021.102256.
Basheer, A. A. 2018a. “Chemical chiral pollution: Impact on the society and science and need of the regulations in the 21st century.” Chirality 30 (4): 402–406. https://doi.org/10.1002/chir.22808.
Basheer, A. A. 2018b. “New generation nano-adsorbents for the removal of emerging contaminants in water.” J. Mol. Liq. 261 (85): 583–593. https://doi.org/10.1016/j.molliq.2018.04.021.
Basheer, A. A., and I. Ali. 2018. “Stereoselective uptake and degradation of (±)-o, p-DDD pesticide stereomers in water-sediment system.” Chirality 30 (9): 1088–1095. https://doi.org/10.1002/chir.22989.
Bhuyar, P., D. D. Hong, E. Mandia, M. H. A. Rahim, G. P. Maniam, and N. Govindan. 2020. “Salinity reduction from poly-chem-industrial wastewater by using microalgae (Chlorella sp.) collected from coastal region of peninsular Malaysia.” J. Biol. Med. Open Access 1 (1): 105.
Bilad, M. R., V. Discart, D. Vandamme, I. Foubert, K. Muylaert, and I. F. Vankelecom. 2014. “Coupled cultivation and pre-harvesting of microalgae in a membrane photobioreactor (MPBR).” Bioresour. Technol. 155 (May): 410–417. https://doi.org/10.1016/j.biortech.2013.05.026.
Boonchai, R., and G. Seo. 2015. “Microalgae membrane photobioreactor for further removal of nitrogen and phosphorus from secondary sewage effluent.” Korean J. Chem. Eng. 32 (10): 2047–2052. https://doi.org/10.1007/s11814-015-0043-9.
Borowitzka, M. A. 2016. “Algal physiology and large-scale outdoor cultures of microalgae.” In The physiology of microalgae, 601–652. Berlin: Springer.
Buonocore, E., S. Mellino, G. De Angelis, G. Liu, and S. Ulgiati. 2018. “Life cycle assessment indicators of urban wastewater and sewage sludge treatment.” Ecol. Indic. 94 (5): 13–23. https://doi.org/10.1016/j.ecolind.2016.04.047.
Cho, S., T. T. Luong, D. Lee, Y. K. Oh, and T. Lee. 2011. “Reuse of effluent water from a municipal wastewater treatment plant in microalgae cultivation for biofuel production.” Bioresour. Technol. 102 (18): 8639–8645. https://doi.org/10.1016/j.biortech.2011.03.037.
Costache, T. A., F. G. Acien Fernandez, M. M. Morales, J. M. Fernández-Sevilla, I. Stamatin, and E. Molina. 2013. “Comprehensive model of microalgae photosynthesis rate as a function of culture conditions in photobioreactors.” Appl. Microbiol. Biotechnol. 97 (17): 7627–7637. https://doi.org/10.1007/s00253-013-5035-2.
Delavari Amrei, H., B. Nasernejad, R. Ranjbar, and S. Rastegar. 2014. “An integrated wavelength-shifting strategy for enhancement of microalgal growth rate in PMMA-and polycarbonate-based photobioreactors.” Eur. J. Phycol. 49 (3): 324–331. https://doi.org/10.1080/09670262.2014.919030.
de Souza Leite, L., M. T. Hoffmann, and L. A. Daniel. 2019. “Microalgae cultivation for municipal and piggery wastewater treatment in Brazil.” J. Water Process Eng. 31 (8): 100821. https://doi.org/10.1016/j.jwpe.2019.100821.
Devriese, M., V. Tsakaloudi, I. Garbayo, R. León, C. Vílchez, and J. Vigara. 2001. “Effect of heavy metals on nitrate assimilation in the eukaryotic microalga Chlamydomonas reinhardtii.” Plant Physiol. Biochem. 39 (5): 443–448. https://doi.org/10.1016/S0981-9428(01)01257-8.
Fallahi, A., N. Hajinajaf, O. Tavakoli, and M. H. Sarrafzadeh. 2020. “Cultivation of mixed microalgae using municipal wastewater: Biomass productivity, nutrient removal, and biochemical content.” Iran. J. Biotechnol. 18 (4): e2586. https://doi.org/10.30498/IJB.2020.2586.
Fallahi, A., F. Rezvani, H. Asgharnejad, E. Khorshidi, N. Hajinajaf, and B. Higgins. 2021. “Interactions of microalgae-bacteria consortia for nutrient removal from wastewater: A review.” Chemosphere 272 (Jun): 129878. https://doi.org/10.1016/j.chemosphere.2021.129878.
Ghimire, A., G. Kumar, P. Sivagurunathan, S. Shobana, G. D. Saratale, H. W. Kim, V. Luongo, G. Esposito, and R. Munoz. 2017. “Bio-hythane production from microalgae biomass: Key challenges and potential opportunities for algal bio-refineries.” Bioresour. Technol. 241 (5): 525–536. https://doi.org/10.1016/j.biortech.2017.05.156.
Gupta, S., S. B. Pawar, and R. A. Pandey. 2019. “Current practices and challenges in using microalgae for treatment of nutrient rich wastewater from agro-based industries.” Sci. Total Environ. 687 (Jun): 1107–1126. https://doi.org/10.1016/j.scitotenv.2019.06.115.
Habibi, A., G. A. Nematzadeh, H. D. Amrei, and A. Teymouri. 2019. “Effect of light/dark cycle on nitrate and phosphate removal from synthetic wastewater based on BG11 medium by Scenedesmus sp.” 3 Biotech 9 (4): 1–9. https://doi.org/10.1007/s13205-019-1679-7.
Hajinajaf, N., A. Mehrabadi, and O. Tavakoli. 2021. “Practical strategies to improve harvestable biomass energy yield in microalgal culture: A review.” Biomass Bioenergy 145 (9): 105941. https://doi.org/10.1016/j.biombioe.2020.105941.
Hashemi, A., M. Moslemi, F. Pajoum Shariati, and H. Delavari Amrei. 2020a. “Beta-carotene production within Dunaliella salina cells under salt stress condition in an indoor hybrid helical-tubular photobioreactor.” Can. J. Chem. Eng. 98 (1): 69–74. https://doi.org/10.1002/cjce.23577.
Hashemi, A., F. Pajoum Shariati, and A. Heydarinasab. 2021. “The effect of instantaneous and slow-release salt stress methods on beta-carotene production within Dunaliella Salina Cells.” Iran. J. Chem. Chem. Eng. 40 (5): 1642–1652. https://doi.org/10.30492/ijcce.2020.107691.3581.
Hashemi, A., F. Pajoum Shariati, E. Sohani, S. Azizi, S. Z. Hosseinifar, and H. Delavari Amrei. 2020b. “CO2 biofixation by Synechococcus elongatus from the power plant flue gas under various light–dark cycles.” Clean Technol. Environ. Policy 22 (8): 1735–1743. https://doi.org/10.1007/s10098-020-01912-0.
Hashemi, S. A., F. Pajoum Shariati, H. Delavari Amrei, and A. Heydarinasab. 2019. “Growth pattern and -carotene production of Dunaliella salina cells in different salinities.” J. Food Technol. Nutrition 16 (4): 45–50.
Holakoo, L., G. Nakhla, A. S. Bassi, and E. K. Yanful. 2007. “Long term performance of MBR for biological nitrogen removal from synthetic municipal wastewater.” Chemosphere 66 (5): 849–857. https://doi.org/10.1016/j.chemosphere.2006.06.026.
Hosseini, M. K., F. P. Shariati, P. K. Hosseini, S. Azizi, and A. Hashemi. 2019. “The effect of polymeric granule as mechanical cleaning technology on membrane fouling in a hybrid microalgal membrane photobioreactor (HMPBR).” In Proc., 6th MEMTEK Int. Symp. on Membrane Technologies and Applications, 18–20. Boca Raton, FL: CRC Press.
Jacob-Lopes, E., C. H. G. Scoparo, L. M. C. F. Lacerda, and T. T. Franco. 2009. “Effect of light cycles (night/day) on CO2 fixation and biomass production by microalgae in photobioreactors.” Chem. Eng. Process. Process Intensif. 48 (1): 306–310. https://doi.org/10.1016/j.cep.2008.04.007.
Keramati, A., S. Azizi, A. Hashemi, and F. Pajoum Shariati. 2021. “Effects of flashing light–emitting diodes (LEDs) on membrane fouling in a reciprocal membrane photobioreactor (RMPBR) to assess nitrate and phosphate removal from whey wastewater.” J. Appl. Phycol. 33 (3): 1513–1524. https://doi.org/10.1007/s10811-021-02388-1.
Khan, S., M. Shahnaz, N. Jehan, S. Rehman, M. T. Shah, and I. Din. 2013. “Drinking water quality and human health risk in Charsadda district, Pakistan.” J. Cleaner Prod. 60 (2): 93–101. https://doi.org/10.1016/j.jclepro.2012.02.016.
Kunjapur, A. M., and R. B. Eldridge. 2010. “Photobioreactor design for commercial biofuel production from microalgae.” Ind. Eng. Chem. Res. 49 (8): 3516–3526. https://doi.org/10.1021/ie901459u.
Lee, K., and C. G. Lee. 2001. “Effect of light/dark cycles on wastewater treatments by microalgae.” Biotechnol. Bioprocess Eng. 6 (3): 194–199. https://doi.org/10.1007/BF02932550.
Luo, Y., P. Le-Clech, and R. K. Henderson. 2017. “Simultaneous microalgae cultivation and wastewater treatment in submerged membrane photobioreactors: A review.” Algal Res. 24 (Oct): 425–437. https://doi.org/10.1016/j.algal.2016.10.026.
Luo, Y., P. Le-Clech, and R. K. Henderson. 2020. “Assessing the performance of membrane photobioreactors (MPBR) for polishing effluents containing different types of nitrogen.” Algal Res. 50 (Feb): 102013. https://doi.org/10.1016/j.algal.2020.102013.
Mantovani, M., F. Marazzi, R. Fornaroli, M. Bellucci, E. Ficara, and V. Mezzanotte. 2020. “Outdoor pilot-scale raceway as a microalgae-bacteria sidestream treatment in a WWTP.” Sci. Total Environ. 710 (Sep): 135583. https://doi.org/10.1016/j.scitotenv.2019.135583.
Mohseni, A., L. Fan, and F. A. Roddick. 2021. “Impact of microalgae species and solution salinity on algal treatment of wastewater reverse osmosis concentrate.” Chemosphere 285 (2): 131487. https://doi.org/10.1016/j.chemosphere.2021.131487.
Mujtaba, G., M. Rizwan, G. Kim, and K. Lee. 2018. “Removal of nutrients and COD through co-culturing activated sludge and immobilized Chlorella vulgaris.” Chem. Eng. J. 343 (Jul): 155–162. https://doi.org/10.1016/j.cej.2018.03.007.
Palmer, E. T., C. J. Poor, C. Hinman, and J. D. Stark. 2013. “Nitrate and phosphate removal through enhanced bioretention media: Mesocosm study.” Water Environ. Res. 85 (9): 823–832. https://doi.org/10.2175/106143013X13736496908997.
Pholchan, M. K., J. D. C. Baptista, R. J. Davenport, and T. P. Curtis. 2010. “Systematic study of the effect of operating variables on reactor performance and microbial diversity in laboratory-scale activated sludge reactors.” Water Res. 44 (5): 1341–1352. https://doi.org/10.1016/j.watres.2009.11.005.
Praveen, P., Y. Guo, H. Kang, C. Lefebvre, and K. C. Loh. 2018. “Enhancing microalgae cultivation in anaerobic digestate through nitrification.” Chem. Eng. J. 354 (Dec): 905–912. https://doi.org/10.1016/j.cej.2018.08.099.
Rawat, I., R. R. Kumar, T. Mutanda, and F. Bux. 2013. “Biodiesel from microalgae: A critical evaluation from laboratory to large scale production.” Appl. Energy 103 (Mar): 444–467. https://doi.org/10.1016/j.apenergy.2012.10.004.
Renuka, N., A. Sood, S. K. Ratha, R. Prasanna, and A. S. Ahluwalia. 2013. “Evaluation of microalgal consortia for treatment of primary treated sewage effluent and biomass production.” J. Appl. Phycol. 25 (5): 1529–1537. https://doi.org/10.1007/s10811-013-9982-x.
Rodolfi, L., G. Chini Zittelli, N. Bassi, G. Padovani, N. Biondi, G. Bonini, and M. R. Tredici. 2009. “Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor.” Biotechnol. Bioeng. 102 (1): 100–112. https://doi.org/10.1002/bit.22033.
Santos, A., and S. Judd. 2010. “The fate of metals in wastewater treated by the activated sludge process and membrane bioreactors: A brief review.” J. Environ. Monit. 12 (1): 110–118. https://doi.org/10.1039/B918161J.
Sepehri, A., M. H. Sarrafzadeh, and M. Avateffazeli. 2020. “Interaction between Chlorella vulgaris and nitrifying-enriched activated sludge in the treatment of wastewater with low C/N ratio.” J. Cleaner Prod. 247 (Feb): 119164. https://doi.org/10.1016/j.jclepro.2019.119164.
Shi, J., B. Podola, and M. Melkonian. 2007. “Removal of nitrogen and phosphorus from wastewater using microalgae immobilized on twin layers: An experimental study.” J. Appl. Phycol. 19 (5): 417–423. https://doi.org/10.1007/s10811-006-9148-1.
Skouteris, G., D. Hermosilla, P. López, C. Negro, and Á. Blanco. 2012. “Anaerobic membrane bioreactors for wastewater treatment: A review.” Chem. Eng. J. 198 (Aug): 138–148. https://doi.org/10.1016/j.cej.2012.05.070.
Sun, S., Z. Ge, Y. Zhao, C. Hu, H. Zhang, and L. Ping. 2016. “Performance of CO2 concentrations on nutrient removal and biogas upgrading by integrating microalgal strains cultivation with activated sludge.” Energy 97 (Feb): 229–237. https://doi.org/10.1016/j.energy.2015.12.126.
Sun, S. P., C. P. I. Nàcher, B. Merkey, Q. Zhou, S. Q. Xia, D. H. Yang, J. H. Sun, and B. F. Smets. 2010. “Effective biological nitrogen removal treatment processes for domestic wastewaters with low C/N ratios: A review.” Environ. Eng. Sci. 27 (2): 111–126. https://doi.org/10.1089/ees.2009.0100.
Taziki, M., H. Ahmadzadeh, M. A. Murry, and S. R. Lyon. 2015. “Nitrate and nitrite removal from wastewater using algae.” Curr. Biotechnol. 4 (4): 426–440. https://doi.org/10.2174/2211550104666150828193607.
Ting, H., L. Haifeng, M. Shanshan, Y. Zhang, L. Zhidan, and D. Na. 2017. “Progress in microalgae cultivation photobioreactors and applications in wastewater treatment: A review.” Int. J. Agric. Biol. Eng. 10 (1): 1–29. https://doi.org/10.3965/j.ijabe.20171001.2705.
Van den Broeck, R., J. Van Dierdonck, P. Nijskens, C. Dotremont, P. Krzeminski, J. H. J. M. Van der Graaf, J. B. Van Lier, J. F. M. Van Impe, and I. Y. Smets. 2012. “The influence of solids retention time on activated sludge bioflocculation and membrane fouling in a membrane bioreactor (MBR).” J. Membr. Sci. 401 (Jan): 48–55. https://doi.org/10.1016/j.memsci.2012.01.028.
Vaneeckhaute, C., V. Lebuf, E. Michels, E. Belia, P. A. Vanrolleghem, F. M. Tack, and E. Meers. 2017. “Nutrient recovery from digestate: Systematic technology review and product classification.” Waste Biomass Valorization 8 (1): 21–40. https://doi.org/10.1007/s12649-016-9642-x.
Verhuelsdonk, M., K. Glas, and H. Parlar. 2021. Long-term operation of a pilot-scale membrane bioreactor treating brewery wastewater: Relaxation as a method for detection of membrane fouling.” J. Environ. Eng. 147 (4): 04021005. https://doi.org/10.1061/(ASCE)EE.1943-7870.0001862.
Wang, L., J. Liu, Q. Zhao, W. Wei, and Y. Sun. 2016. “Comparative study of wastewater treatment and nutrient recycle via activated sludge, microalgae and combination systems.” Bioresour. Technol. 211 (Mar): 1–5. https://doi.org/10.1016/j.biortech.2016.03.048.
Winkler, M. K. H., C. Meunier, O. Henriet, J. Mahillon, M. E. Suárez-Ojeda, G. Del Moro, M. De Sanctis, C. Di Iaconi, and D. G. Weissbrodt. 2018. “An integrative review of granular sludge for the biological removal of nutrients and recalcitrant organic matter from wastewater.” Chem. Eng. J. 336 (Mar): 489–502. https://doi.org/10.1016/j.cej.2017.12.026.
Yu, H., J. Kim, and C. Lee. 2019. “Potential of mixed-culture microalgae enriched from aerobic and anaerobic sludges for nutrient removal and biomass production from anaerobic effluents.” Bioresour. Technol. 280 (May): 325–336. https://doi.org/10.1016/j.biortech.2019.02.054.
Zhou, W., M. Min, Y. Li, B. Hu, X. Ma, Y. Cheng, Y. Liu, P. Chen, and R. Ruan. 2012. “A hetero-photoautotrophic two-stage cultivation process to improve wastewater nutrient removal and enhance algal lipid accumulation.” Bioresour. Technol. 110 (Apr): 448–455. https://doi.org/10.1016/j.biortech.2012.01.063.
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© 2022 American Society of Civil Engineers.
History
Received: Feb 27, 2022
Accepted: Jul 25, 2022
Published online: Sep 30, 2022
Published in print: Dec 1, 2022
Discussion open until: Feb 28, 2023
ASCE Technical Topics:
- Activated sludge
- Bioreactors
- Chemical compounds
- Chemical processes
- Chemicals
- Chemistry
- Environmental engineering
- Membranes
- Municipal wastewater
- Nitrates
- Oxygen demand
- Phosphate
- Pollutants
- Salts
- Sludge
- Structural engineering
- Structural members
- Structural systems
- Waste management
- Waste treatment
- Wastes
- Wastewater management
- Wastewater treatment
- Water treatment
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