Effects of Environmental Factors on the Spatial Succession of the Bacterial Community in Municipal Solid-Waste Landfills
Publication: Journal of Environmental Engineering
Volume 148, Issue 7
Abstract
Secondary pollution control and rapid stabilization of municipal solid waste (MSW) landfills are closely associated with bacterial communities. The spatial variations in bacterial community structure and the environmental factors controlling it were studied by the 16S rRNA gene sequencing of MSW sampled at different depths in a large sanitary landfill (LSL) and a medium non-sanitary landfill (MNSL), respectively. The total nitrogen (TN) and total phosphorus (TP) content in the LSL and MNSL samples displayed a downward trend as the depth increased. However, the organic matter (OM) content followed the opposite trend. Diversity of microbes was highest in the middle layer of both the LSL and MNSL. Firmicutes and Proteobacteria were the dominant phylum both in the LSL and MNSL. In the LSL, the dominant bacteria genera succeeded from Psychrobacter, Pseudomonas to Clostridium, Savagea. However, in the MNSL, the genera succeeded from Uncultured, Pleomorphomonas to Hydrogenispora, Acetomicrobium. The type of landfill and the different environmental factors control the succession of the bacterial community in LSL and MNSL. The pH, OM, TN, and TP will change in the landfill as the landfill depth increased. The impact degree of landfill depth on pH, OM, TN, and TP content in the LSL was 15.3%, 16.1%, 4.9%, and 4.2%, respectively. These values were lower than those in the MNSL. In addition, depth and OM had the strongest correlations but no significance with the dominant bacteria both in the LSL () and MNSL (). This indicated that bacterial community succession was mainly dependent on space, time, and MSW characteristics in different types of landfills.
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Data Availability Statement
All data generated or used during the study appear in the published article. These data were presented in tables or figures.
Acknowledgments
This research was funded by the Science and technology program for Distinguished Young Scientists of Sichuan Province (Grant No. 2020JDJQ0053), the Major Special Science and Technology Project of Sichuan Province (Grant No. 2019YFS0509), the State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Independent Research Project (Grant No. SKLGP 2019Z009).
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Journal of Environmental Engineering
Volume 148 • Issue 7 • July 2022
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© 2022 American Society of Civil Engineers.
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Received: Nov 9, 2021
Accepted: Feb 1, 2022
Published online: Apr 28, 2022
Published in print: Jul 1, 2022
Discussion open until: Sep 28, 2022
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