Bioenergy Production and Slaughterhouse Wastewater Treatment in a Column-Type Anaerobic Sequencing Batch Reactor without Any External Mixer or Gas or Liquid Recirculation
Publication: Journal of Environmental Engineering
Volume 147, Issue 3
Abstract
The performance of a column-type anaerobic sequencing batch reactor (ASBR) with a high height-to-diameter () ratio of 10 was evaluated while treating slaughterhouse wastewater. The reactor was operated without any external mixers, gas recirculation, or liquid recirculation. The chemical oxygen demand (COD) removal efficiency was about 70%–75% at different hydraulic retention times (HRTs) (48, 32, 24, and ). Biogas production was stable at biogas, comprising about 60%–70% methane between 2 and . A high ratio promoted good retention of denser sludge, and increased the production of biogas. The novel bioreactor without any external mixing and biogas/liquid recirculation is a promising technique, which can save considerable energy compared with conventional bioreactors if the biogas generated is maintained properly inside the ASBR. Additionally, microbial analysis targeting the 16S ribosomal RNA (16S rRNA) gene indicated the dominance of methanogens, which comprised Methanosaetaceae (23.07%), Methanobacteriaceae (2.05%), Methanomicrobiaceae (1.85%), Methanoregulaceae (0.82%), Methanocorpusculaceae (0.64%), and Methanospirillaceae (0.42%).
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Data Availability Statement
Some or all data, models, or code that support the findings of this study are available from the corresponding author upon reasonable request.
Acknowledgments
The corresponding author (Dr. Farrukh Basheer, Aligarh Muslim Univ.) thanks the sponsors of this project for their financial support, namely the Early Career Research Award, Science and Engineering Research Board (SERB), Department of Science and Technology (DST), New Delhi, India, Sanction order ECR/2016/00162, June 23, 2017.
References
Akil, K., S. Jayanthi, and V. Uma. 2012. “Biogas potential of distillery wastewater in an anaerobic sequencing batch biofilm reactor.” Indian J. Environ. Prot. 32 (11): 918–925. https://doi.org/10.1080/09593330.2014.964334.
Alkaya, E., and G. N. Demirer. 2011. “Anaerobic acidification of sugar-beet processing wastes: Effect of operational parameters.” Biomass Bioenergy 35 (1): 32–39. https://doi.org/10.1016/j.biombioe.2010.08.002.
Alsouleman, K., B. Linke, J. Klang, M. Klocke, N. Krakat, and S. Theuerl. 2016. “Reorganisation of a mesophilic biogas microbiome as response to a stepwise increase of ammonium nitrogen induced by poultry manure supply.” Bioresour. Technol. 208 (May): 200–204. https://doi.org/10.1016/j.biortech.2016.02.104.
APHA (American Public Health Association). 2005. Standard methods for the examination of water and wastewater. 21st ed. Washington, DC: APHA.
Aziz, A., F. Basheer, A. Sengar, S. U. Khan, and I. H. Farooqi. 2019. “Biological wastewater treatment (anaerobic-aerobic) technologies for safe discharge of treated slaughterhouse and meat processing wastewater.” Sci. Total Environ. 686 (Oct): 681–708. https://doi.org/10.1016/j.scitotenv.2019.05.295.
Bustillo-Lecompte, C. F., and M. Mehrvar. 2015. “Slaughterhouse wastewater characteristics, treatment, and management in the meat processing industry: A review on trends and advances.” J. Environ. Manage. 161 (Sep): 287–302. https://doi.org/10.1016/j.jenvman.2015.07.008.
Caporaso, J. G., et al. 2010. “QIIME allows analysis of high-throughput community sequencing data.” Nat. Methods 7 (5): 335–336. https://doi.org/10.1038/nmeth.f.303.
Central Pollution Control Board of India. 2017. “Revised comprehensive industry document on slaughterhouses.” Accessed October 9, 2018. http://cpcb.nic.in/openpdffile.php?id=TGF0ZXN0RmlsZS8xNzVfMTUxMTI2NDE0MV9tZWRpYXBob3RvODkzOS5wZGY=.
Chen, Y., J. J. Cheng, and K. S. Creamer. 2008. “Inhibition of anaerobic digestion process: A review.” Bioresour. Technol. 99 (10): 4044–4064. https://doi.org/10.1016/j.biortech.2007.01.057.
Chiang, C. F., and R. R. Dague. 1992. “Effects of reactor configuration and biomass activity on the performance of upflow static media anaerobic reactors.” Water Environ. Res. 64 (2): 141–149. https://doi.org/10.2175/WER.64.2.7.
Del Nery, V., E. Pozzi, M. H. R. Z. Damianovic, M. R. Domingues, and M. Zaiat. 2008. “Granules characteristics in the vertical profile of a full-scale upflow anaerobic sludge blanket reactor treating poultry slaughterhouse wastewater.” Bioresour. Technol. 99 (6): 2018–2024. https://doi.org/10.1016/j.biortech.2007.03.019.
Edgar, R. C. 2010. “Search and clustering orders of magnitude faster than BLAST.” Bioinformatics 26 (19): 2460–2461. https://doi.org/10.1093/bioinformatics/btq461.
Granada, C. E., et al. 2018. “Biogas from slaughterhouse wastewater anaerobic digestion is driven by the archaeal family Methanobacteriaceae and bacterial families Porphyromonadaceae and Tissierellaceae.” Renewable Energy 118 (Apr): 840–846. https://doi.org/10.1016/j.renene.2017.11.077.
Guiot, S. R., A. Pauss, D. Bourque, M. El Housseini, L. Lavoie, C. Beaulieu, and R. Samson, 1988. “Effect of upflow liquid velocity on granule size distribution in an upflow anaerobic bed-filter (UBF) reactor.” In Proc., 5th Int. Symp. on Anaerobic Digestion, edited by A. Tilche and A. Rozzi, 121–124. Bologna, Italy: Monduzzi Editore.
Hamawand, I., and C. Baillie. 2015. “Anaerobic digestion and biogas potential: Simulation of lab and industrial-scale processes.” Energies 8 (1): 454–474. https://doi.org/10.3390/en8010454.
Jabari, L., H. Gannoun, E. Khelifi, J.-L. Cayol, J.-J. Godon, M. Hamdi, and M.-L. Fardeaub. 2016. “Bacterial ecology of abattoir wastewater treated by an anaerobic digestor.” Braz. J. Microbiol. 47 (1): 73–84. https://doi.org/10.1016/j.bjm.2015.11.029.
Jeong, T.-Y., G.-C. Cha, Y.-C. Seo, C. Jeon, and S. S. Choi. 2008. “Effect of COD/sulfate ratios on batch anaerobic digestion using waste activated sludge.” J. Ind. Eng. Chem. 14 (5): 693–697. https://doi.org/10.1016/j.jiec.2008.05.006.
Johns, M. R. 1995. “Developments in wastewater treatment in the meat processing industry: A review.” Bioresour. Technol. 54 (3): 203–216. https://doi.org/10.1016/0960-8524(95)00140-9.
Koster, I. W., and G. Lettinga. 1988. “Anaerobic digestion at extreme ammonia concentrations.” Biol. Wastes 25 (1): 51–59. https://doi.org/10.1016/0269-7483(88)90127-9.
Kuglarz, M., B. Mrowiec, and J. Bohdziewicz. 2011. “Influence of kitchen biowaste addition on the effectiveness of animal manure digestion in continuous condition.” In Proc., Research and Application of New Technologies in Wastewater Treatment and Municipal Solid Waste Disposal in Ukraine, Sweden and Poland, 31–40. Seattle: Allen Institute for AI.
Lefebvre, O., N. Vasudevan, M. Torrijos, K. Thanasekaran, and R. Moletta. 2006. “Anaerobic digestion of tannery soak liquor with an aerobic post-treatment.” Water Res. 40 (7): 1492–1500. https://doi.org/10.1016/j.watres.2006.02.004.
Lens, P. N. L., D. Korthout, J. B. van Lier, L. W. Hulshoff Pol, and G. Lettinga. 2001. “Effect of the liquid upflow velocity on thermophilic sulphate reduction in acidifying granular sludge reactors.” Environ. Technol. 22 (2): 183–193. https://doi.org/10.1080/09593332208618294.
Masse, D. I., and L. Masse. 2000. “Agriculture and agri-food Canada contribution.” Can. Agric. Eng. 42 (3): 131–137.
Masse, D. I., and L. Masse. 2001. “The effect of temperature on slaughterhouse wastewater treatment in anaerobic sequencing batch reactors.” Bioresour. Technol. 76 (2): 91–98. https://doi.org/10.1016/S0960-8524(00)00105-X.
Maurina, G. Z., L. M. Rosa, L. L. Beal, C. Baldasso, J. R. Gimenez, A. P. Torres, and M. P. Sousa. 2014. “Effect of internal recirculation velocity in an anaerobic sequencing batch reactor (ASBR).” Braz. J. Chem. Eng. 31 (4): 895–903. https://doi.org/10.1590/0104-6632.20140314s00002895.
McCarty, P. L., and D. P. Smith. 1986. “Anaerobic wastewater treatment.” Environ. Sci. Technol. 20 (12): 1200–1206. https://doi.org/10.1021/es00154a002.
Michelan, R., T. R. Zimmer, J. A. D. Rodrigues, S. M. Ratusznei, D. de Moraes, M. Zaiat, and E. Foresti. 2009. “Effect of impeller type and mechanical agitation on the mass transfer and power consumption aspects of ASBR operation treating synthetic wastewater.” J. Environ. Manage. 90 (3): 1357–1364. https://doi.org/10.1016/j.jenvman.2008.08.003.
Mittal, G. S. 2006. “Treatment of wastewater from abattoirs before land application—A review.” Bioresour. Technol. 97 (9): 1119–1135. https://doi.org/10.1016/j.biortech.2004.11.021.
O’Flaherty, V., P. N. L. Lens, D. de Beer, and E. Colleran. 1997. “Effect of feed composition and upflow velocity on aggregate characteristics in anaerobic upflow reactors.” Appl. Microbiol. Biotechnol. 47 (2): 102–107. https://doi.org/10.1007/s002530050896.
Ozgun, H., M. E. Ersahin, Y. Tao, H. Spanjers, and J. B. van Lier. 2013. “Effect of upflow velocity on the effluent membrane fouling potential in membrane coupled upflow anaerobic sludge blanket reactors.” Bioresour. Technol. 147 (Nov): 285–292. https://doi.org/10.1016/j.biortech.2013.08.039.
Pérez-Pérez, T., G. T. Correia, W. H. Kwong, I. Pereda-Reyes, D. Oliva-Merencio, and M. Zaiat. 2017. “Effects of the support material addition on the hydrodynamic behavior of an anaerobic expanded granular sludge bed reactor.” J. Environ. Sci. 54 (Apr): 224–230. https://doi.org/10.1016/j.jes.2016.02.011.
Pinho, S. C., S. M. Ratusznei, J. A. D. Rodrigues, E. Foresti, and M. Zaiat. 2005. “Feasibility of treating partially soluble wastewater in anaerobic sequencing batch biofilm reactor (ASBBR) with mechanical stirring.” Bioresour. Technol. 96 (4): 517–519. https://doi.org/10.1016/j.biortech.2004.05.027.
Rajagopal, R., D. I. Massé, and G. Singh. 2013. “A critical review on inhibition of anaerobic digestion process by excess ammonia.” Bioresour. Technol. 143 (Sep): 632–641. https://doi.org/10.1016/j.biortech.2013.06.030.
Reino, C., and J. Carrera. 2017. “Low-strength wastewater treatment in an anammox UASB reactor: Effect of the liquid upflow velocity.” Chem. Eng. J. 313 (Apr): 217–225. https://doi.org/10.1016/j.cej.2016.12.051.
Rivière, D., V. Desvignes, E. Pelletier, S. Chaussonnerie, S. Guermazi, J. Weissenbach, T. Li, P. Camacho, and A. Sghir. 2009. “Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge.” ISME J. 3 (6): 700–714. https://doi.org/10.1038/ismej.2009.2.
Sarti, A., B. S. Fernandes, M. Zaiat, and E. Foresti. 2007. “Anaerobic sequencing batch reactors in pilot-scale for domestic sewage treatment.” Desalination 216 (1–3): 174–182. https://doi.org/10.1016/j.desal.2007.01.007.
Sengar, A., A. Aziz, I. H. Farooqi, and F. Basheer. 2018. “Development of denitrifying phosphate accumulating and anammox micro-organisms in anaerobic hybrid reactor for removal of nutrients from low strength domestic sewage.” Bioresour. Technol. 267 (Nov): 149–157. https://doi.org/10.1016/j.biortech.2018.07.023.
Shao, X., D. Peng, Z. Teng, and X. Ju. 2008. “Treatment of brewery wastewater using anaerobic sequencing batch reactor (ASBR).” Bioresour. Technol. 99 (8): 3182–3186. https://doi.org/10.1016/j.biortech.2007.05.050.
Show, K.-Y., Y. Yan, H. Yao, H. Guo, T. Li, D.-Y. Show, J.-S. Chang, and D.-J. Lee. 2020. “Anaerobic granulation: A review of granulation hypotheses, bioreactor designs and emerging green applications.” Bioresour. Technol. 300 (Jan): 122751. https://doi.org/10.1016/j.biortech.2020.122751.
Sousa, D. Z., H. Smidt, M. M. Alves, and A. J. M. Stams. 2009. “Ecophysiology of syntrophic communities that degrade saturated and unsaturated long-chain fatty acids.” FEMS Microbiol. Ecol. 68 (3): 257–272. https://doi.org/10.1111/j.1574-6941.2009.00680.x.
Sung, S., and R. R. Dague. 1995. “Laboratory studies on the anaerobic sequencing batch reactor.” Water Environ. Res. 67 (3): 294–301. https://doi.org/10.2175/106143095X131501.
Vannecke, T. P. W., D. R. A. Lampens, G. A. Ekama, and E. I. P. Volcke. 2015. “Evaluation of the 5 and 8 pH point titration methods for monitoring anaerobic digesters treating solid waste.” Environ. Technol. 36 (7): 861–869. https://doi.org/10.1080/09593330.2014.964334.
William, T., and T. Shea. 2013. “Anaerobic digestion and co-digestion optimization.” In Proc., Ohio Water Environment Association Biosolids Conf. Columbus, OH: Ohio Water Environment Association.
Zheng, Z., J. Liu, X. Yuan, X. Wang, W. Zhu, F. Yang, and Z. Cui. 2015. “Effect of dairy manure to switchgrass co-digestion ratio on methane production and the bacterial community in batch anaerobic digestion.” Appl. Energy 151 (Aug): 249–257. https://doi.org/10.1016/j.apenergy.2015.04.078.
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Published In
Journal of Environmental Engineering
Volume 147 • Issue 3 • March 2021
Copyright
© 2021 American Society of Civil Engineers.
History
Received: Jun 2, 2020
Accepted: Nov 12, 2020
Published online: Jan 15, 2021
Published in print: Mar 1, 2021
Discussion open until: Jun 15, 2021
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