Biomitigation of and Extraction of Biomolecules Using Leptolyngbya sp.
Publication: Journal of Environmental Engineering
Volume 145, Issue 6
Abstract
A cyanobacterial strain, Leptolyngbya sp., was used for biomitigation due to its effectiveness for bioconversion of and higher yield of biomass. In the present research study, biomitigation was done using cyanobacteria cultivated in scrubbed water containing sodium carbonate in equilibrium with various concentrations of (5%–30%), at different pH conditions (7–11), with different inoculum sizes (5%–12.5%) and at various temperatures (20°C–35°C). The biomass obtained after biomitigation was analyzed for its carbohydrate, protein, and lipid contents. The maximum biomitigation of was found to be in the range of 74.44±0.89% to 22.98±0.56% for concentration of 5% to 30%. The maximum productivities were found as follows: biomass, ; lipid, ; carbohydrate, ; and protein, at pH 9, 15% , inoculum size of 12.5%, and a temperature of 25°C. As the flue gas contains in the range of 10% to 15%, the present cyanobacterial strain could be viewed as a valuable aid for mitigation in the future.
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Acknowledgments
The authors are thankful to the Department of Science and Technology Govt. of India [Sanction Order No.: DST/IS-STAC/-SR-191/14(G) dated 24.11.2014] for providing the project funds in carrying out this research study. Authors are also thankful to Dr. Gautam Bandyopadhyay, Associate Professor, Management Studies Department, National Institute of Technology Durgapur, India for helping with the statistical analysis.
References
Anjos, M., B. D. Fernandes, A. A. Vicente, J. A. Teixeira, and G. Dragone. 2013. “Optimization of bio-mitigation by Chlorella vulgaris.” Bioresour. Technol. 139: 149–154. https://doi.org/10.1016/j.biortech.2013.04.032.
Banerjee, S., M. Pal, J. Chakrabarty, C. Petibios, R. R. Paul, A. Giri, and J. Chatterjee. 2015. “Fourier-transform-infrared-spectroscopy based spectral-biomarker selection towards optimum diagnostic differentiation of oral leukoplakia and cancer.” Anal. Bioanal. Chem. 407 (26): 7935–7943. https://doi.org/10.1007/s00216-015-8960-3.
Bhakta, J. N., S. Lahiri, J. K. Pittman, and B. B. Jana. 2015. “Carbon dioxide sequestration in wastewater by a consortium of elevated carbon dioxide-tolerant microalgae.” J. Util. 10: 105–112. https://doi.org/10.1016/j.jcou.2015.02.001.
Bilanovic, D., A. Andargatchew, T. Kroeger, and G. Shelef. 2009. “Freshwater and marine microalgae sequestering of at different C and N concentrations—Response surface methodology analysis.” Energy Convers. Manage. 50 (2): 262–267. https://doi.org/10.1016/j.enconman.2008.09.024.
Boonburapong, B., S. Laloknam, N. Yamada, A. Incharoensakdi, and T. Takabe. 2012. “Sodium dependent uptake of glutamate by novel ApGltS enhanced growth under salt stress of halo tolerant cyanobacterium Aphanothece halophytica.” Biosci. Biotechnol. Biochem. 76 (9): 1702–1707. https://doi.org/10.1271/bbb.120309.
Cheah, W. Y., P. L. Show, J. S. Chang, T. C. Ling, and J. C. Juan. 2015. “Review: Biosequestration of atmospheric and flue gas-containing by microalgae.” Bioresour. Technol. 184: 190–201. https://doi.org/10.1016/j.biortech.2014.11.026.
Chisti, Y. 2007. “Biodiesel from microalgae.” Biotechnol. Adv. 25 (3): 294–306. https://doi.org/10.1016/j.biotechadv.2007.02.001.
Clesceri, L. S., A. E. Greenberg, and R. R. Trussell. 1996. Standard methods for the examination of water and wastewater. Washington, DC: APHA, AWWA, and WPCF.
Fernando, A. L., S. Boléo, B. Barbosa, J. Costa, J. Lino, C. Tavares, S. Sidella, M. P. Duarte, and B. Mendes. 2014. “How sustainable is the production of energy crops in heavy metal contaminated soils?” In Proc., 22nd European Biomass Conf. and Exhibition, Setting the Course for a Biobased Economy, edited by C. Hoffmann, D. Baxter, K. Maniatis, A. Grassi, and P. Helm, 1593–1596. Hamburg, Germany: ETA Renewable Energies.
Goli, A., A. Shamiri, A. Talaiekhozani, N. Eshtiaghi, N. Aghamohammadi, and M. K. Aroua. 2016. “An overview of biological processes and their potential for capture.” J. Environ. Manage. 183: 41–58. https://doi.org/10.1016/j.jenvman.2016.08.054.
Hsueh, H. T., H. Chu, and S. T. Yu. 2007. “A batch study on the bio-fixation of carbon dioxide in the absorbed solution from a chemical wet scrubber by hot spring and marine alga.” Chemosphere 66 (5): 878–886. https://doi.org/10.1016/j.chemosphere.2006.06.022.
Jebali, A., F. G. Acién, S. Sayadi, and E. Molina-Grima. 2018. “Utilization of centrate from urban wastewater plants for the production of Scenedesmus sp. in a raceway-simulating reactor.” J. Environ. Manage. 211: 112–124. https://doi.org/10.1016/j.jenvman.2018.01.043.
Kargupta, W., A. Ganesh, and S. Mukherji. 2015. “Estimation of carbon dioxide sequestration potential of microalgae grown in a batch photobioreactor.” Bioresour. Technol. 180: 370–375. https://doi.org/10.1016/j.biortech.2015.01.017.
Kole, C. 2007. Technical crops: Genomic mapping and molecular breeding in plants. Vol. 6. University Park, PA: Pennsylvania State Univ.
Kumar, A., S. Ergas, X. Yuan, A. Sahu, Q. Zhang, J. Dewulf, F. X. Malcata, and H. van Langenhove. 2010. “Enhanced fixation and biofuel production via microalgae: Recent developments and future directions.” Trends Biotechnol. 28 (7): 371–380. https://doi.org/10.1016/j.tibtech.2010.04.004.
Kumar, K., C. N. Dasgupta, B. Nayak, P. Lindblad, and D. Das. 2011. “Development of suitable photobioreactor for sequestration addressing global warming using green algae and cyanobacteria.” Bioresour. Technol. 102 (8): 4945–4953. https://doi.org/10.1016/j.biortech.2011.01.054.
Kushwaha, D., S. Saha, and S. Dutta. 2014. “Enhanced biomass recovery during phycoremediation of Cr(VI) using cyanobacteria and prospect of biofuel production.” Ind. Eng. Chem. Res. 53 (51): 19754–19764. https://doi.org/10.1021/ie501311c.
Lam, M. K., and K. T. Lee. 2013. “Effect of carbon source towards the growth of bio-mitigation and biodiesel production.” Int. J. Greenh. Gas Cont. 14: 169–176. https://doi.org/10.1016/j.ijggc.2013.01.016.
Langley, N. M., S. T. L. Harrison, and R. P. van Hille. 2012. “A critical evaluation of supplementation to algal systems by direct injection.” Biochem. Eng. J. 68: 70–75. https://doi.org/10.1016/j.bej.2012.07.013.
Luklema, L. 1969. “Factors affecting pH changes in alkaline wastewater treatment—I.” Water Res. 3 (12): 913–930. https://doi.org/10.1016/0043-1354(69)90075-X.
Maxwell, D. P., S. Falk, C. C. Trick, and N. P. A. Huner. 1994. “Growth at low temperature mimics high-light acclimation in Chlorella vulgaris.” Plant Physiol. 105 (2): 535–543. https://doi.org/10.1104/pp.105.2.535.
Mokashi, K., V. Shetty, S. A. George, and G. Sibi. 2016. “Sodium bicarbonate as inorganic carbon source for higher biomass and lipid production integrated carbon capture in Chlorella vulgaris.” Achiev. Life Sci. 10 (1): 111–117. https://doi.org/10.1016/j.als.2016.05.011.
Moreira, D., and J. C. M. Pires. 2016. “Atmospheric capture by algae: Negative carbon dioxide emission path.” Bioresour. Technol. 215: 371–379. https://doi.org/10.1016/j.biortech.2016.03.060.
Ramanan, R., K. Kannan, A. Deshkar, R. Yadav, and T. Chakrabarti. 2010. “Enhanced algal sequestration through calcite deposition by Chlorella sp. and Spirulina platensis in a mini-raceway pond.” Bioresour. Technol. 101 (8): 2616–2622. https://doi.org/10.1016/j.biortech.2009.10.061.
Roberts, D. A., N. A. Paul, M. I. Bird, and R. de Nys. 2015. “Bioremediation for coal-fired power stations using microalgae.” J. Environ. Manage. 153: 25–32. https://doi.org/10.1016/j.jenvman.2015.01.036.
Rosgaard, L., A. J. de Porcellinis, J. H. Jacobsen, N. U. Frigaard, and Y. Sakuragi. 2012. “Bioengineering of carbon fixation, biofuels, and biochemicals in cyanobacteria and plants.” J. Biotechnol. 162 (1): 134–147. https://doi.org/10.1016/j.jbiotec.2012.05.006.
Ruangsomboon, S. 2012. “Effect of light, nutrient, cultivation time and salinity on lipid production of newly isolated strain of the green microalga Botryococcus braunii KMITL 2.” Bioresour. Technol. 109: 261–265. https://doi.org/10.1016/j.biortech.2011.07.025.
Sen, S., S. Dutta, S. Guhathakurata, J. Chakrabarty, S. Nandi, and A. Dutta. 2017. “Removal of Cr(VI) using a cyanobacterial consortium and assessment of biofuel production.” Int. Biodeter. Biodegr. 119: 211–224. https://doi.org/10.1016/j.ibiod.2016.10.050.
Singh, S. P., and P. Sing. 2015. “Effect of temperature and light on the growth of algae species: A review.” Renew. Sust. Energ. Rev. 50: 431–444. https://doi.org/10.1016/j.rser.2015.05.024.
Tang, D., W. Han, P. Li, X. Miao, and J. Zhong. 2011. “ biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different levels.” Bioresour. Technol. 102 (3): 3071–3076. https://doi.org/10.1016/j.biortech.2010.10.047.
Thakurta, S. G., M. Aakula, J. Chakrabarty, and S. Dutta. 2018. “Bioremediation of phenol from synthetic and real wastewater using Leptolyngbya sp.: A comparison and assessment of biofuel production.” 3 Biotech 8 (4): 206. https://doi.org/10.1007/s13205-018-1229-8.
Touloupakis, E., B. Cicchi, A. M. S. Benavides, and G. Torzillo. 2016. “Effect of high pH on growth of Synechocystis sp. PCC 6803 cultures and their contamination by golden algae (Poterioochromonas sp.).” Appl. Microbiol. Biotechnol. 100 (3): 1333–1341. https://doi.org/10.1007%2Fs00253-015-7024-0.
Upendar, G., A. N. Mistry, R. Das, S. G. Thakurata, J. Chakrabarty, K. C. Ghanta, and S. Dutta. 2018a. “Carbon dioxide biofixation using microorganisms and assessment of biofuel production.” Environ. Prog. Sustain. Energy 37 (5): 1594–1600. https://doi.org/10.1002/ep.12835.
Upendar, G., S. Singh, J. Chakrabarty, K. C. Ghanta, S. Dutta, and A. Dutta. 2018b. “Sequestration of carbon dioxide and production of biomolecules using cyanobacteria.” J. Environ. Manage. 218: 234–244. https://doi.org/10.1016/j.jenvman.2018.04.031.
Wang, B., Y. Li, N. Wu, and C. Q. Lan. 2008. “ bio-mitigation using microalgae.” Appl. Microbiol. Biotechnol. 79 (5): 707–718. https://doi.org/10.1007/s00253-008-1518-y.
Winck, F. V., D. O. P. Melo, D. M. Riaño-Pachón, M. C. M. Martins, C. Caldana, and A. F. G. Barrios. 2016. “Analysis of sensitive Chlamydomonas reinhardtii through genomic scale modelling and experimental validation.” Front. Plant Sci. 7: 43. https://doi.org/10.3389%2Ffpls.2016.00043.
Yadav, G., A. Karemore, S. K. Dash, and R. Sen. 2015. “Performance evaluation of a green process for microalgal sequestration in closed photobioreactor using flue gas generated in-situ.” Bioresour. Technol. 191: 399–406. https://doi.org/10.1016/j.biortech.2015.04.040.
Yen, H. W., and D. E. Brune. 2007. “Anaerobic co-digestion of algal sludge and waste paper to produce methane.” Bioresour. Technol. 98 (1): 130–134. https://doi.org/10.1016/j.biortech.2005.11.010.
Yoo, C., S. Y. Jun, J. Y. Lee, C. Y. Ahn, and H. M. Oh. 2010. “Selection of microalgae for lipid production under high levels carbon dioxide.” Bioresour. Technol. 101 (1): S71–S74. https://doi.org/10.1016/j.biortech.2009.03.030.
Zeng, X., M. K. Danquah, X. D. Chen, and Y. Lu. 2011. “Microalgae bioengineering: From fixation to biofuel production.” Renew. Sustain. Energy Rev. 15 (6): 3252–3260. https://doi.org/10.1016/j.rser.2011.04.014.
Zhu, B., G. Chen, X. Cao, and D. Wei. 2017. “Molecular characterization of sequestration and assimilation in microalgae and its biotechnological applications.” Bioresour. Technol. 244 (2): 1207–1215. https://doi.org/10.1016/j.biortech.2017.05.199.
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©2019 American Society of Civil Engineers.
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Received: May 2, 2018
Accepted: Nov 13, 2018
Published online: Mar 22, 2019
Published in print: Jun 1, 2019
Discussion open until: Aug 22, 2019
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