Bioremediation of Cr(VI) Using Live Cyanobacteria: Experimentation and Kinetic Modeling
Publication: Journal of Environmental Engineering
Volume 144, Issue 9
Abstract
The removal of Cr(VI) from synthetic wastewater using living cyanobacteria Limnococcus sp. was examined. Characterizations of the native and metal loaded cells were done using scanning electron microscopy, energy-dispersive spectroscopy, and Fourier transform infrared spectroscopy. The operating condition was optimized using one-factor-at-a-time analysis. Maximum Cr(VI) removal (63.23%) was achieved with initial Cr(VI) concentration , pH 9.0, inoculum size 10% [volume per volume (v/v)], and incubation period 16 days. The distribution of Cr(VI) on the cell surface through biosorption and in the intracellular space through bioaccumulation were also determined. The logistic model was used to design the variation of growth of the biomass with time. Maximum specific growth rate () was determined to be . The maximum carrying capacity of the synthetic wastewater observed was with initial Cr(VI) concentration of . An empirical power-law model was also developed to predict the interactive effect of the initial Cr(VI) concentration, pH, inoculum size, and incubation time with the cell dry mass (CDM), percentage removal of Cr(VI), and lipid content.
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Acknowledgments
The authors are grateful to the National Institute of Technology, Durgapur, West Bengal, India, for providing financial support for the research activities.
References
Abigail, M. E. A., M. S. Samuel, and R. Chidambaram. 2015. “Hexavalent chromium biosorption studies using Penicillium griseofulvum MSR1 a novel isolate from tannery effluent site: Box-Behnken optimization, equilibrium, kinetics and thermodynamic studies.” J. Taiwan Inst. Chem. E. 49: 156–164. https://doi.org/10.1016/j.jtice.2014.11.026.
Ahalya, N., T. V. Ramachandra, and R. D. Kanamadi. 2003. “Biosorption of heavy metals.” Res. J. Chem. Environ. 7 (4): 71–79.
Ahluwalia, S., and S. D. Goyal. 2007. “Microbial and plant derived biomass for removal of heavy metals from wastewater.” Bioresour. Technol. 98 (12): 2243–2257. https://doi.org/10.1016/j.biortech.2005.12.006.
Anjana, K., A. Kaushik, B. Kiran, and R. Nisha. 2007. “Biosorption of Cr(VI) by immobilized biomass of two indigenous strains of cyanobacteria isolated from metal contaminated soil.” J. Hazard. Mater. 148 (1–2): 383–386. https://doi.org/10.1016/j.jhazmat.2007.02.051.
Antunes, W. M., A. S. Luna, C. A. Henriques, and A. C. A. da Costa. 2003. “An evaluation of biosorption by a brown seaweed under optimized condition.” Electron. J. Biotechnol. 6 (3): 174–184. https://doi.org/10.2225/vol6-issue3-fulltext-5.
ATSDR (Agency for Toxic Substances and Disease Registry). 2017. “ATSDR’s substance priority list.” Accessed April 28, 2017. https://www.atsdr.cdc.gov/spl/.
Baei, M. S., G. D. Najafpour, H. Younesi, F. Tabandeh, H. Issazadeh, and M. Khodabandeh. 2011. “Growth kinetic parameters and biosynthesis of polyhydroxybutyrate in Cupriavidus necator DSMZ 545 on selected substrates.” Chem. Ind. Chem. Eng. Q. 17 (1): 1–8. https://doi.org/10.2298/CICEQ100216043B.
Biswas, G., S. G. Thakurta, J. Chakrabarty, K. Adhikari, and S. Dutta. 2018. “Evaluation of fluoride bioremediation and production of biomolecules by living cyanobacteria under fluoride stress condition.” Ecotoxicol. Environ. Saf. 148: 26–36. https://doi.org/10.1016/j.ecoenv.2017.10.019.
Bligh, E. G., and W. J. Dyer. 1959. “A rapid method of total lipid extraction and purification.” Can. J. Biochem. Physiol. 37 (8): 911–917. https://doi.org/10.1139/o59-099.
Chatterjee, S., S. Dutta, M. Mukherjee, P. Ray, and S. Basu. 2015. “Studies on removal of lead(II) by alginate immobilized bromelain (AIB).” Desalination Water Treat. 56 (2): 409–424. https://doi.org/10.1080/19443994.2014.936514.
Collins, L., D. Alvarez, and A. Chauhan. 2014. Microbial biodegradation and bioremediation. London: Elsevier.
Dhanasekar, R., T. Viruthagiri, and P. L. Sabarathinam. 2003. “Poly (3-hydroxy butyrate) synthesis from a mutant strain Azotobacter vinelandii utilizing glucose in a batch reactor.” Biochem. Eng. J. 16 (1): 1–8. https://doi.org/10.1016/S1369-703X(02)00176-6.
Focardi, S., M. Pepi, G. Landi, S. Gasperini, M. Ruta, P. D. Biasio, and S. E. Focardi. 2012. “Hexavalent chromium reduction by whole cells and cell free extract of the moderate halophilic bacterial strain Halomonas sp. TA-04.” Int. Biodeterior. Biodegrad. 66 (1): 63–70. https://doi.org/10.1016/j.ibiod.2011.11.003.
Gabr, R. M., S. H. A. Hassan, and A. A. M. Shoreit. 2008. “Biosorption of lead and nickel by living and non-living cells of Pseudomonas aeruginosa ASU 6a.” Int. Biodeterior. Biodegrad. 62 (2): 195–203. https://doi.org/10.1016/j.ibiod.2008.01.008.
Guha, H., K. Jayachandran, and F. Maurrasse. 2001. “Kinetics of chromium (VI) reduction by a type strain Shewanella alga under different growth conditions.” Environ. Pollut. 115 (2): 209–218. https://doi.org/10.1016/S0269-7491(01)00108-7.
Gupta, V. K., and A. Rastogi. 2009. “Biosorption of hexavalent chromium by raw and acid-treated green alga Oedogonium hatei from aqueous solutions.” J. Hazard. Mater. 163 (1): 396–402. https://doi.org/10.1016/j.jhazmat.2008.06.104.
Hörcsik, Z., V. Oláh, Á. Balogh, I. Mészáros, L. Simon, and G. Lakatos. 2006. “Effect of chromium (VI) on growth, element and photosynthetic pigment composition of Chlorella pyrenoidosa.” Acta Biol. Szeged. 50 (1–2): 19–23.
Joo, J. H., S. H. A. Hassan, and S. E. Oh. 2010. “Comparative study of biosorption of by Pseudomonas aeruginosa and Bacillus cereus.” Int. Biodeterior. Biodegrad. 64 (8): 734–741. https://doi.org/10.1016/j.ibiod.2010.08.007.
Karale, R. S., D. V. Wadkar, and P. B. Nangare. 2007. “Removal and recovery of hexavalent chromium from industrial waste water by precipitation with due consideration to cost optimization.” J. Environ. Res. Develop. 2 (2): 209–216.
Kumar, K. S., H.-U. Dahms, E.-J. Won, J.-S. Lee, and K.-H. Shin. 2015. “Microalgae—A promising tool for heavy metal remediation.” Ecotoxicol. Environ. Saf. 113: 329–352. https://doi.org/10.1016/j.ecoenv.2014.12.019.
Kundu, N., M. Pal, and S. Saha. 2008. “East Kolkata Wetlands: A resource recovery system through productive activities.” In Proc., Taal 2007: The 12thWorld Lake Conf., edited by M. Sengupta and R. Dalwani. New Delhi, India: Ministry of Environment and Forests.
Kushwaha, D., S. Saha, and S. Dutta. 2014. “Enhanced biomass recovery during phycoremediation of Cr(VI) using cyanobacteria and prospect of biofuel production.” Industrial Eng. Chem. Res. 53 (51): 19754–19764. https://doi.org/10.1021/ie501311c.
Liu, T., Z.-L. Wang, L. Zhao, and X. Yang. 2012. “Enhanced -nanoparticles beads for hexavalent chromium removal from wastewater.” Chem. Eng. J. 189–190: 196–202. https://doi.org/10.1016/j.cej.2012.02.056.
Mahmoudi, M., M. S. Baei, G. D. Najafpour, F. Tabandeh, and H. Eisazadeh. 2010. “Kinetic model for polyhydroxybutyrate (PHB) production by Hydrogenophaga pseudoflava and verification of growth conditions.” Afr. J. Biotechnol. 9 (21): 3151–3157.
Malachowski, L., J. L. Stair, and J. A. Holcombe. 2004. “Immobilized peptides/amino acids on solid supports for metal remediation.” Pure Appl. Chem. 76 (4): 777–787. https://doi.org/10.1351/pac200476040777.
Markou, G., and E. Nerantzis. 2013. “Microalgae for high-value compounds and biofuels production: A review with focus on cultivation under stress conditions.” Biotechnol. Adv. 31 (8): 1532–1542. https://doi.org/10.1016/j.biotechadv.2013.07.011.
Matsunaga, T., H. Takeyama, T. Nakao, and A. Yamazawa. 1999. “Screening of marine microalgae for bioremediation of cadmium-polluted seawater.” J. Biotechnol. 70 (1–3): 33–38. https://doi.org/10.1016/S0168-1656(99)00055-3.
Miranda, J., G. Krishnakumar, and A. D’Silva. 2012. “Removal of from aqueous system by live Oscillatoria laete-virens (Crouan and Crouan) Gomont isolated from industrial effluents.” World J. Microbiol. Biotechnol. 28 (10): 3053–3065. https://doi.org/10.1007/s11274-012-1115-1.
Narayani, M., and K. V. Shetty. 2013. “Chromium-resistant bacteria and their environmental condition for hexavalent chromium removal: A review.” Crit. Rev. Environ. Sci. Technol. 43 (9): 955–1009. https://doi.org/10.1080/10643389.2011.627022.
Pradhan, D., L. B. Sukla, M. Sawyer, and P. K. S. M. Rahman. 2017. “Recent bioreduction of hexavalent chromium in wastewater treatment: A review.” J. Ind. Eng. Chem. 55: 1–20. https://doi.org/10.1016/j.jiec.2017.06.040.
Preetha, B., and T. Viruthagiri. 2007. “Bioaccumulation of chromium(VI), copper(II) and nickel(II) ions by growing Rhizopus arrhizus.” Biochem. Eng. J. 34 (2): 131–135. https://doi.org/10.1016/j.bej.2006.11.022.
Qu, Y., X. Zhang, J. Xu, W. Zhang, and Y. Guo. 2014. “Removal of hexavalent chromium from wastewater using magnetotactic bacteria.” Sep. Purif. Technol. 136: 10–17. https://doi.org/10.1016/j.seppur.2014.07.054.
Rahman, M. U., S. Gul, and M. Z. U. Haq. 2007. “Reduction of chromium(VI) by locally isolated Pseudomonas sp. C-171.” Turkish J. Biol. 31 (3): 161–166.
Rangabhashiyam, S., and N. Selvaraju. 2015. “Adsorptive remediation of hexavalent chromium from synthetic wastewater by a natural and activated Sterculia guttata shell.” J. Mol. Liq. 207: 39–49. https://doi.org/10.1016/j.molliq.2015.03.018.
Rangasamy, S., G. Purushothaman, B. Alagirisamy, and M. Santiago. 2015. “Chromium contamination in soil and groundwater due to tannery wastes disposal at Vellore district of Tamil Nadu.” Int. J. Environ. Sci. 6 (1): 114–124.
Raychaudhuri, S., M. Mishra, P. Nandy, and A. R. Thakur. 2008. “Waste management: A case study of ongoing traditional practices at east Calcutta Wetland.” Am. J. Agric. Biol. Sci. 3 (1): 315–320. https://doi.org/10.3844/ajabssp.2008.315.320.
Rich, G., and K. Cherry. 1987. Hazardous waste treatment technologies. New York: Pudvan Publishers.
Sen, S., S. Dutta, S. Guhathakurata, J. Chakrabarty, S. Nandi, and A. Dutta. 2017. “Removal of Cr(VI) using a cyanobacterial consortium and assessment of biofuel production.” Int. Biodeter. Biodegr. 119: 211–224. https://doi.org/10.1016/j.ibiod.2016.10.050.
Sepehr, M. N., S. Nasseri, M. M. Asadi, and K. Yaghmalan. 2005. “Chromium bioremoval from tannery industries effluent by Aspergillus oryzae.” Iran. J. Environ. Health Sci. Eng. 2 (4): 267–272.
Shamsollahi, H. R., M. Alimohammadi, R. Nabizadeh, S. Nazmara, and A. H. Mahvi. 2015. “Measurement of mycrocystine-LR in water samples using improved HPLC method.” Glob. J. Health. Sci. 7 (2): 66–70. https://doi.org/10.5539/gjhs.v7n2p66.
Shukla, A., Y. H. Zhang, P. Dubey, J. L. Margrave, and S. S. Shukla. 2002. “The role of sawdust in the removal of unwanted materials from water.” J. Hazard. Mater. 95 (1–2): 137–152. https://doi.org/10.1016/S0304-3894(02)00089-4.
Shuler, M. L., and F. Kargi. 2008. Bioprocess engineering. New Delhi, India: Prentice-Hall of India.
Silva, B., H. Figueiredo, C. Quintelas, I. C. Neves, and T. Tavares. 2012. “Improved biosorption for Cr(VI) reduction and removal by Arthrobacter viscosus using zeolite.” Int. Biodeterior. Biodegrad. 74: 116–123. https://doi.org/10.1016/j.ibiod.2012.05.026.
Sukumar, M. 2010. “Reduction of hexavalent chromium by Rhizopus oryzae.” Afr. J. Environ. Sci. Technol. 4 (7): 412–418.
Tang, D., W. Han, P. Li, X. Miao, and J. Zhong. 2011. “ levels.” Bioresour. Technol. 102 (3): 3071–3076. https://doi.org/10.1016/j.biortech.2010.10.047.
Thatoi, H., S. Das, J. Mishra, B. P. Rath, and N. Das. 2014. “Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: A review.” J. Environ. Manage. 146: 383–399. https://doi.org/10.1016/j.jenvman.2014.07.014.
Torokne, A., R. Vasdinnyei, and B. M. Asztalos. 2007. “A rapid microbiotest for the detection of cyanobacterial toxins.” Environ. Toxicol. 22 (1): 64–68. https://doi.org/10.1002/tox.20235.
USEPA. 2017. “Chromium in drinking water.” Accessed April 28, 2017. http://www.epa.gov/dwstandardsregulations/chromium-drinking-water.
Volesky, B., and Z. R. Holan. 1995. “Biosorption of heavy metals.” Biotechnol. Prog. 11 (3): 235–250. https://doi.org/10.1021/bp00033a001.
Yoo, C., G. Choi, S. Kim, and H. Oh. 2013. “Ettlia sp. YC001 showing high growth rate and lipid content under high .” Bioresour. Technol. 127: 482–488. https://doi.org/10.1016/j.biortech.2012.09.046.
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Published In
Journal of Environmental Engineering
Volume 144 • Issue 9 • September 2018
Copyright
©2018 American Society of Civil Engineers.
History
Received: Sep 26, 2017
Accepted: Mar 21, 2018
Published online: Jul 9, 2018
Published in print: Sep 1, 2018
Discussion open until: Dec 9, 2018
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