Technical Papers
Feb 16, 2018

Effect of Flow Rate Increase on the Performance of a Pilot-Scale Biological Nutrient Removal Reactor

Publication: Journal of Environmental Engineering
Volume 144, Issue 5

Abstract

Nutrient removal in two identical pilot-scale reactors is assessed at influent flow rates of 30, 45, and 60  L/min. The bacteria community structure of nitrifiers and denitrifiers and the protein:polysaccharide ratio (PN/PS) in extracellular polymeric substances (EPS) are monitored at each flow rate. Except for the chemical oxygen demand (COD), the reactor performance in terms of ammonia and phosphorous removal begins to deteriorate at the 45  L/min influent flow rate. Nitrobacter are more dominant than are nitrite-oxidizing bacteria (NOB). The PN/PS ratio in sludge EPS significantly decreases as the solids retention time (SRT) decreases from 9.3 to 2.2 days. The PN/PS ratio temporarily increases in response to an increase in flow rate before normalizing to a steadier value.

Get full access to this article

View all available purchase options and get full access to this article.

Acknowledgments

The authors acknowledge Natural Sciences and Engineering Research Council of Canada (NSERC) Collaborative Research and Development (CRD) Grants and EPCOR Water Services for their financial supports.

References

Bradford, M. M. (1976). “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.” Anal. Biochem., 72(1–2), 248–254.
Bridgewater, L., and Rice, E. W. (2012). Standard methods for the examination of water and wastewater, American Public Health Association, Washington, DC.
Chung, C. M., Cho, K. W., Kim, Y. J., Yamamoto, K., and Chung, T. H. (2012). “Enhanced biological nitrogen removal in MLE combined with post-denitrification process and EF clarifier.” Bioprocess. Biosyst. Eng., 35(4), 503–511.
Chung, Y. J., Choi, H. N., Cho, J. B., and Park, S. K. (2004). “Treatment of swine wastewater using MLE process and membrane bio-reactor.” Water Sci. Technol., 49(5–6), 443–450.
Dionisi, H. M., Layton, A. C., Harms, G., Gregory, I. R., Robinson, K. G., and Sayler, G. S. (2002). “Quantification of Nitrosomonas oligotropha-like ammonia-oxidizing bacteria and Nitrospira spp. from full-scale wastewater treatment plants by competitive PCR.” Appl. Environ. Microbiol., 68(1), 245–253.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956). “Colorimetric method for determination of sugars and related substances.” Anal. Chem., 28(3), 350–356.
Franklin, L., Abu-Orf, M., Bowden, G., and Pfrang, W. (2014). “Suspended growth biological treatment processes.” Chapter 8, Wastewater engineering: Treatment and resource recovery, Metcalf & Eddy, G. Tchobanoglous, H. D. Stensel, R. Tsuchihashi, and F. L. Burton, eds., McGraw-Hill Education, New York.
Gentile, M. E., Jessup, C. M., Nyman, J. L., and Criddle, C. S. (2007). “Correlation of functional instability and community dynamics in denitrifying dispersed-growth reactors.” Appl. Environ. Microbiol., 73(3), 680–690.
Ghosh, S., and LaPara, T. M. (2004). “Removal of carbonaceous and nitrogenous pollutants from a synthetic wastewater using a membrane-coupled bioreactor.” J. Ind. Microbiol. Biotechnol., 31(8), 353–361.
Graham, D. W., Knapp, C. W., Van Vleck, E. S., Bloor, K., Lane, T. B., and Graham, C. E. (2007). “Experimental demonstration of chaotic instability in biological nitrification.” ISME J., 1(5), 385–393.
Harms, G., et al. (2003). “Real-time PCR quantification of nitrifying bacteria in a municipal wastewater treatment plant.” Environ. Sci. Technol., 37(2), 343–351.
Henry, S., Baudoin, E., López-Gutiérrez, J. C., Martin-Laurent, F., Brauman, A., and Philippot, L. (2004). “Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR.” J. Microbiol. Methods, 59(3), 327–335.
Higgins, M. J., and Novak, J. T. (1997). “Characterization of exocellular protein and its role in bioflocculation.” J. Environ. Eng., 479–485.
Holakoo, L., Nakhla, G., Bassi, A. S., and Yanful, E. K. (2007). “Long term performance of MBR for biological nitrogen removal from synthetic municipal wastewater.” Chemosphere, 66(5), 849–857.
Hu, X., Xie, L., Shim, H., Zhang, S., and Yang, D. (2014). “Biological nutrient removal in a full scale anoxic/anaerobic/aerobic/pre-anoxic-MBR plant for low C/N ratio municipal wastewater treatment.” Chin. J. Chem. Eng., 22(4), 447–454.
Huang, C., Shi, Y., Sheng, Z., El-Din, M. G., and Liu, Y. (2017). “Characterization of microbial communities during start-up of integrated fixed-film activated sludge (IFAS) systems for the treatment of oil sands process-affected water (OSPW).” Biochem. Eng. J., 122, 123–132.
Ibarbalz, F. M., Figuerola, E. L. M., and Erijman, L. (2013). “Industrial activated sludge exhibit unique bacterial community composition at high taxonomic ranks” Water Res., 47(11), 3854–3864.
Janga, N., Ren, X., Kim, G., Ahn, C., Cho, J., and Kim, I. S. (2007). “Characteristics of soluble microbial products and extracellular polymeric substances in the membrane bioreactor for water reuse.” Desalination, 202(1), 90–98.
Kim, Y. M., Lee, D. S., Park, C., Park, D., and Park, J. M. (2011). “Effects of free cyanide on microbial communities and biological carbon and nitrogen removal performance in the industrial activated sludge process.” Water Res., 45(3), 1267–1279.
Li, H., Zhang, Y., Yang, M., and Kamagata, Y. (2013). “Effects of hydraulic retention time on nitrification activities and population dynamics of a conventional activated sludge system.” Front. Environ. Sci. Eng., 7(1), 43–48.
Li, X. Y., and Yang, S. F. (2007). “Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge.” Water Res., 41(5), 1022–1030.
Liang, Z., Das, A., Beerman, D., and Hu, Z. (2010). “Biomass characteristics of two types of submerged membrane bioreactors for nitrogen removal from wastewater.” Water Res., 44(11), 3313–3320.
Liao, B., Allen, D., Droppo, I., Leppard, G., and Liss, S. (2001). “Surface properties of sludge and their role in bioflocculation and settleability.” Water Res., 35(2), 339–350.
Liu, H., and Fang, H. H. P. (2002). “Extraction of extracellular polymeric substances (EPS) of sludges.” J. Biotechnol., 95(3), 249–256.
McTavish, H., Fuchs, J. A., and Hooper, A. B. (1993). “Sequence of the gene coding for ammonia monooxygenase in Nitrosomonas europaea.” J. bacterial., 175(8), 2436–2444.
Muyzer, G., de Waal, E. C., and Uitterlinden, A. G. (1993). “Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.” Appl. Environ. Microbiol., 59(3), 695–700.
Ni, B.-J., Yu, H.-Q., and Sun, Y.-J. (2008). “Modeling simultaneous autotrophic and heterotrophic growth in aerobic granules.” Water Res., 42(6–7), 1583–1594.
Ouyang, K., and Liu, J. (2009). “Effect of sludge retention time on sludge characteristics and membrane fouling of membrane bioreactor.” J. Environ. Sci., 21(10), 1329–1335.
Pan, S., Tay, J.-H., He, Y.-X., and Tay, S. T.-L. (2004). “The effect of hydraulic retention time on the stability of aerobically grown microbial granules.” Lett. Appl. Microbiol., 38(2), 158–163.
Rodriguez-Sanchez, A., Gonzalez-Martinez, A., Martinez-Toledo, M., Garcia-Ruiz, M., Osorio, F., and Gonzalez-Lopez, J. (2014). “The effect of influent characteristics and operational conditions over the performance and microbial community structure of partial nitritation reactors.” Water, 6(7), 1905–1924.
Sesay, M. L., Özcengiz, G., and Dilek Sanin, F. (2006). “Enzymatic extraction of activated sludge extracellular polymers and implications on bioflocculation.” Water Res., 40(7), 1359–1366.
Shen, J.-P., Zhang, L.-M., Di, H. J., and He, J.-Z. (2012). “A review of ammonia-oxidizing bacteria and archaea in Chinese soils.” Front. Microbiol., 3, 296.
Sheng, G.-P., Yu, H.-Q., and Li, X.-Y. (2010). “Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: A review.” Biotechnol. Adv., 28(6), 882–894.
Sheng, Z., Mohammed, A., and Liu, Y. (2016). “Stability of full-scale engineered ecosystem under disturbance: Response of an activated sludge biological nutrient removal reactor to high flow rate condition.” Int. Biodeterior. Biodegrad., 109, 88–95.
Wang, X., Peng, Y., Wang, S., Fan, J., and Cao, X. (2006). “Influence of wastewater composition on nitrogen and phosphorus removal and process control in A2O process.” Bioprocess. Biosyst. Eng., 28(6), 397–404.
Xu, S., Bernards, M., and Hu, Z. (2014a). “Evaluation of anaerobic/anoxic/oxic (A2/O) and reverse A2/O processes in biological nutrient removal.” Water Environ. Res., 86(11), 2186–2193.
Xu, S., Sun, M., Zhang, C., Surampalli, R., and Hu, Z. (2014b). “Filamentous sludge bulking control by nano zero-valent iron in activated sludge treatment systems.” Environ. Sci. Processes Impacts, 16(12), 2721–2728.
Xu, S., Wu, D., and Hu, Z. (2014c). “Impact of hydraulic retention time on organic and nutrient removal in a membrane coupled sequencing batch reactor.” Water Res., 55, 12–20.
Xu, S., Zhang, Y., Sims, A., Bernards, M. T., and Hu, Z. (2013). “Fate and toxicity of melamine in activated sludge treatment systems after a long-term sludge adaptation.” Water Res., 47(7), 2307–2314.
Yu, K., and Zhang, T. (2012). “Metagenomic and metatranscriptomic analysis of microbial community structure and gene expression of activated sludge.” PLoS One, 7(5), e38183.

Information & Authors

Information

Published In

Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 144Issue 5May 2018

History

Received: May 1, 2017
Accepted: Oct 6, 2017
Published online: Feb 16, 2018
Published in print: May 1, 2018
Discussion open until: Jul 16, 2018

Permissions

Request permissions for this article.

Authors

Affiliations

Kingsley Nze [email protected]
M.Sc. Student, Dept. of Civil and Environmental Engineering, 3-133 Markin/CNRL Natural Resources Engineering Facility, Univ. of Alberta, Edmonton, AB, Canada T6G 2W2. E-mail: [email protected]
Shengnan Xu, Ph.D. [email protected]
Postdoctoral, Dept. of Civil and Environmental Engineering, 7-203 Donadeo Innovation Centre for Engineering, Univ. of Alberta, Edmonton, AB, Canada T6G 1H9; Dept. of Civil and Environmental Engineering, Shantou Univ., Shantou, Guangdong 515063, China. E-mail: [email protected]
M.Sc. Student, Dept. of Civil and Environmental Engineering, 3-133 Markin/CNRL Natural Resources Engineering Facility, Univ. of Alberta, Edmonton, AB, Canada T6G 2W2. E-mail: [email protected]
Abdul Mohammed [email protected]
P.Eng.
Senior Specialist in Wastewater Treatment, EPCOR Water Services, Inc., 10977 50 St. NW, Edmonton, AB, Canada T6A 2E9. E-mail: [email protected]
Yang Liu, Ph.D. [email protected]
P.Eng.
Associate Professor, Dept. of Civil and Environmental Engineering, 7-203 Donadeo Innovation Centre for Engineering, Univ. of Alberta, Edmonton, AB, Canada T6G 1H9 (corresponding author). E-mail: [email protected]

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share