Development of a Superparamagnetic Laccase Nanobiocatalyst for the Enzymatic Biotransformation of Xenobiotics
Publication: Journal of Environmental Engineering
Volume 144, Issue 3
Abstract
The development of nanotechnology has provided a range of diverse nanoscale carriers that can be potentially applied for enzyme immobilization. Among the different types of support, magnetic nanoparticles (mNPs) have received increasing attention because they can be easily recovered from the reaction medium by applying an external magnetic field. In the present study, silica-coated magnetic nanoparticles () were prepared, characterized, and used for covalent immobilization of laccase from Trametes versicolor. Different concentrations of the functionalization agent (3-aminopropyltriethoxysilane), cross-linker (glutaraldehyde), and laccase allowed a maximum enzyme loading of mNP. Several factors, including pH, T, presence of inactivating compounds, enzyme stability, and reusability of the support, were evaluated. The oxidative action of the enzyme toward xenobiotics was proven in the formation of the chromogenic radical of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and the biotransformation of the endocrine-disrupting compound Bisphenol A (BPA). The enhancement of catalytic activity and stability of the nanobiocatalyst was evidenced by the increase of ABTS oxidation. Moreover, the superparamagnetic characteristic of the support allowed simple and fast recovery of the nanobiocatalyst. With the development of nanobiocatalyst-driven applications in mind, the development of magnetic nanoparticle synthesis at large scale as well as the design of bioreactors that assure the retention and recovery of the nanobiocatalyst need to addressed.
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Acknowledgments
This work was financially supported by the Spanish Ministry of Economy and Competitiveness (CTQ2013-44762-R and CTQ2016-79461-R, program cofunded by FEDER). The authors (Y. Moldes-Diz, M. Gamallo, G. Eibes, G. Feijoo, J. M. Lema, and M. T. Moreira) belong to the Galician Competitive Research Group GRC 2013-032, program cofunded by FEDER. Y.M.-D. thanks the Spanish Ministry of Economy and Competitiveness for her predoctoral fellowship.
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Published In
Journal of Environmental Engineering
Volume 144 • Issue 3 • March 2018
Copyright
©2018 American Society of Civil Engineers.
History
Received: Apr 17, 2017
Accepted: Aug 25, 2017
Published online: Jan 10, 2018
Published in print: Mar 1, 2018
Discussion open until: Jun 10, 2018
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