Filtration of Glycoprotein-Modified Carboxylated Polystyrene Microspheres as Cryptosporidium Oocysts Surrogates: Effects of Flow Rate, Alum, and Humic Acid
Publication: Journal of Environmental Engineering
Volume 143, Issue 8
Abstract
In this study, a surrogate of Cryptosporidium parvum oocysts was prepared using Cryptosporidium-sized carboxylated polystyrene microspheres and an alpha-1-acid glycoprotein (AGP). The zeta potential of the modified surrogates was found to be similar to that of viable Cryptosporidium parvum. These surrogates were used in laboratory-scale filtration experiments under the effects of flow velocity, alum addition, and humic acid (HA) coating on quartz sand surfaces. Filtration results showed that increased flow rates enhanced the transport of microspheres mostly as a result of increased hydrodynamic force. HA adsorbed on the grain surfaces greatly enhanced the mobility of microspheres in the packed bed, possibly as a result of increased repulsion between microspheres and the media. Alum inhibited the transport of microspheres in the column mostly as a result of the charge neutralization of the microspheres. The transport of microspheres when alum and HA coexisted was almost the same as the condition when alum and HA were absent. Deposition profiles showed a monotonic decline with distance under conditions where no HA was present, while nonmonotonic depositions were observed under conditions where HA was adsorbed on collector surfaces.
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Acknowledgments
The authors acknowledge the Natural Sciences and Engineering Research Council of Canada (NSERC) and EPCOR Water Services Inc. for their financial support.
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Published In
Journal of Environmental Engineering
Volume 143 • Issue 8 • August 2017
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©2017 American Society of Civil Engineers.
History
Received: Jun 16, 2016
Accepted: Nov 1, 2016
Published ahead of print: Mar 30, 2017
Published online: Mar 31, 2017
Published in print: Aug 1, 2017
Discussion open until: Aug 31, 2017
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