Decolorization and Detoxification of Malachite Green by Ganoderma lucidum: Key Operating Parameters and Adsorption Studies
Publication: Journal of Environmental Engineering
Volume 143, Issue 4
Abstract
Ganoderma lucidum’s potential to decolorize and detoxify malachite green (MG) was studied. A Plackett-Burman experimental design to select operational factors and conditions favoring decolorization associated with laccase activity was performed. The most influential variables based on this statistical optimization were temperature, rpm for flask agitation, inoculum percentage, and dye concentration. Based on these features, removal experiments for 96 h were performed. Following thr 96-h removal assays, it was demonstrated that viable biomass at different glucose concentrations was able to remove over 90% MG at a concentration, evidencing a positive correlation with laccase activity. Adsoprtion studies allowed to evidence acid pHs favored MG adsorption, with the Elovich model best describing the chimioadsorption phenomenon. Finally, it was demonstrated that MG () without treatment was highly toxic for Lactuca sativa seed germination index (GI) (). This index increased when seeds were exposed to MG solution after it was treated with G. lucidum viable biomass (VB).
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Acknowledgments
This research was funded by the following grants: Project ID: 00004334 (Expresión a escala de laboratorio de las lacasas recombinantes de Pleurotus ostreatus y Ganoderma lucidum), and Project ID: 00004335 (Evaluación de Ganoderma lucidum, Pleurotus ostreatus y fotocatálisis con para la remoción de colorantes trifenilmetánicos), Pontificia Universidad Javeriana, Bogotá, D.C. Colombia, Grupo de Biotecnología Ambiental e Industrial (GBAI). The authors thank Fiona Raikes and María Lucía Gutiérrez for their English editing.
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Published In
Journal of Environmental Engineering
Volume 143 • Issue 4 • April 2017
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©2016 American Society of Civil Engineers.
History
Received: Feb 22, 2015
Accepted: Sep 6, 2016
Published online: Dec 8, 2016
Published in print: Apr 1, 2017
Discussion open until: May 8, 2017
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