Specific Adsorption of Silver (I) from the Aqueous Phase by Saccharomyces cerevisiae Cells with Enhanced Displaying CueR Protein
Publication: Journal of Environmental Engineering
Volume 142, Issue 9
Abstract
Three types of CueR proteins were anchored on the surface of Saccharomyces cerevisiae cells via surface display technology. The surface-engineered cells showed enhanced adsorption capacity and specificity toward Ag (I) in aqueous phase than control groups. A series of experiments were conducted to investigate the resistance of the surface-engineered cells toward environmental changes including pH, temperature, and eluent. The surface-engineered Saccharomyces cerevisiae cells had the highest adsorption capacity of , which was 87.3% higher than original cells. The engineered cells were able to tolerate an acidic environment ( to 5.0) and high temperature (up to 50°C) with relatively stable adsorption performance. Desorption efficiency was 11% (, 1 mM eluent), which implied the absorbed Ag (I) ions bonded to the cells with high affinity.
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Acknowledgments
This research was supported by the Shenzhen municipal government under grants CXB201111240110A and JCYJ20130329174424934. The authorsalso appreciate Mr. Alex Patton, from Oak Ridge National Laboratory, U.S., for the help in English writing.
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Published In
Journal of Environmental Engineering
Volume 142 • Issue 9 • September 2016
Copyright
© 2015 American Society of Civil Engineers.
History
Received: Sep 3, 2014
Accepted: Jul 9, 2015
Published online: Sep 16, 2015
Discussion open until: Feb 16, 2016
Published in print: Sep 1, 2016
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