Biomineralization of Carbonate by Terrabacter Tumescens for Heavy Metal Removal and Biogrouting Applications
Publication: Journal of Environmental Engineering
Volume 142, Issue 9
Abstract
The ability of Terrabacter tumescens to precipitate carbonate minerals has been demonstrated for the first time. Biomineralization of nickel, copper, lead, cobalt, zinc, cadmium and calcium by T. tumescens and mineral precipitations in both liquid and porous media were investigated. T. tumescens produced the enzyme urease which can hydrolyze urea. Due to this enzymatic reaction, soil pH increased and carbonate was produced, which results in mineralization of the soluble heavy metal ions present in soil water and their ultimate conversion to carbonates. And we studied the process of calcium carbonate precipitation induced by T. tumescens in sand column. The precipitated minerals were studied by scanning electron microscopy and X-ray diffraction, bioaccumulated divalent metal cation were deposited around the cell envelope as rhombohedral, sphere and needle shaped crystalline carbonate compounds. T. tumescens can play important role in heavy metal bioremediation and increase the strength and stiffness of sand and stone in civil engineering applications.
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Acknowledgments
This work was supported by National Natural Science Foundation of China (Project Nos. 50908122 and 51078202).
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Information & Authors
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Published In
Journal of Environmental Engineering
Volume 142 • Issue 9 • September 2016
Copyright
© 2015 American Society of Civil Engineers.
History
Received: Sep 5, 2014
Accepted: Mar 26, 2015
Published online: Jun 18, 2015
Discussion open until: Nov 18, 2015
Published in print: Sep 1, 2016
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