Abstract
It is suggested in this paper that recalcitrant organopollutants can be degraded efficiently by a hemoglobin-catalytic reaction in the presence of hydrogen peroxide (i.e., ). The catalytic mechanism was studied with 5-aminosalicylic acid (5-ASA) as a compound for oxidation. Various evidence suggests that the catalytic mechanism is very similar to those of horseradish peroxidase and lignin peroxidase. The catalytic intermediates are known to oxidize various chemicals, indicating that the intermediates of hemoglobin can nonspecifically degrade many different types of organopollutants. To prove the hypothesis, an attempt was made to remediate polycyclic aromatic hydrocarbon (PAH)-contaminated field soil. The results showed that 98.5% of the PAHs compounds were removed by Day 42 and that seven of the 16 PAHs compounds analyzed were not detectable by the end of the research reported in this paper. Therefore, hemoglobin-catalyzed technology can be considered as a novel technology for remediation of soil contaminated with hazardous organopollutants.
Get full access to this article
View all available purchase options and get full access to this article.
Acknowledgments
The research reported in this paper was supported by the Korea Ministry of Environment (MOE) as Geo- Advanced Innovative Action Project Program.
References
ATSDR (Agency for Toxic Substances and Disease Registry). (1995). Toxicological profile for polycyclic aromatic hydrocarbons, U.S. Dept. of Health and Human Services, Atlanta.
Barr, D., and Aust, S. (1994). “Mechanisms white rot fungi use to degrade pollutants.” Environ. Sci. Technol., 28(2), 78A–87A.
Bengtsson, G., and Zerhouni, P. (2003). “Effects of carbon substrate enrichment and DOC concentration on biodegradation of PAHs in soil.” J. Appl. Microbiol., 94(4), 608–617.
Bolin, C. A., and Smith, S. T. (2013). “Life cycle assessment of creosote-treated wooden railroad crossties in the U.S. with comparisons to concrete and plastic composite railroad crossties.” J. Transp. Technol., 3(2), 149–161.
Chen, S., Stevens, D., and Kang, G. (1999). “Pentachlorophenol and crystal violet degradation in water and soil using heme and hydrogen peroxide.” Water Resour., 33(17), 3657–3665.
Chen, S., Stevens, D., Kang, G., and Hsieh, M. (2006). “Treating soil PCP at optimal conditions using heme and peroxide.” J. Environ. Eng., 704–708.
Crosby, D. G. (1981). “Environmental chemistry of pentachlorophenol.” Pure Appl. Chem., 53(5), 1051–1080.
Fernández-Luqueño, F., Valenzuela-Encinas, C., Marsch, R., Martínez-Suárez, C., Vázquez-Núñez, E., and Dendooven, L. (2011). “Microbial communities to mitigate contamination of PAHs in soil—Possibilities and challenges: A review.” Environ. Sci. Pollut. Res., 18(1), 12–30.
Hadibarata, T. (2009). “Oxidative degradation of by the ligninolytic fungi, interdisciplinary studies on environmental chemistry.” Interdisciplinary studies on environmental chemistry–Environmental research in Asia, Y. Obayashi, T. Isobe, A. Subramanian, S. Suzuki, and S. Tanabe, eds., Terra Scientific, Tokyo, 309–316.
Heikkila, P. R., Hameila, M., Pyy, L., and Raunu, P. (1987). “Exposure to creosote in the impregnation and handling of impregnated wood.” Scand. J. Work Environ. Health, 13(5), 431–437.
Jonsson, S., et al. (2007). “Degradation of polycyclic aromatic hydrocarbons (PAHs) in contaminated soils by Fenton’s reagent: A multivariate evaluation of the importance of soil characteristics and PAH properties.” J. Hazard. Mater., 149(1), 86–96.
Kang, G., Jung, J., Park, K., and Stevens, D. K. (1995). “Mineralization of hazardous chemicals by heme reaction.” Emerging technologies in hazardous waste management, Vol. 7, D. W. Tedder and F. G. Pohland, eds., American Chemical Society, Washington, DC, 407–413.
Lamar, R. T., and Evans, J. W. (1993). “Solid-phase treatment of a pentachlorophenol contaminated soil using lignin degrading fungi.” Environ. Sci. Technol., 27(12), 2566–2571.
Menzie, C., Potocki, B., and Santodonato, J. (1992). “Exposure to carcinogenic PAHs in the environment.” Environ. Sci. Technol., 26(7), 1278–1284.
Nadarajah, N., Van Hamme, J., Pannu, J., Singh, A., and Ward, O. (2002). “Enhanced transformation of polycyclic aromatic hydrocarbons using a combined Fenton’s reagent, microbial treatment and surfactants.” Appl. Microbiol. Biotechnol., 59, 540–544.
Nam, K., Rodriguez, W., and Kukor, J. J. (2001). “Enhanced degradation of polycyclic aromatic hydrocarbons by biodegradation combined with a modified Fenton reaction.” Chemosphere, 45(1), 11–20.
Ortiz-Leon, M., Velasco, L., and Vazquez-Duhalt, R. (1995). “Biocatalytic oxidation of polycyclic aromatic hydrocarbons by hemoglobin and hydrogen peroxide.” Biochem. Biophys. Res. Commun., 215(3), 968–973.
Rentz, J. A., Alvarez, P. J. J., and Schnoor, J. L. (2008). “Benzo[a]pyrene degradation by Sphingomonas yanoikuyae JAR02.” Environ. Pollut., 151(3), 669–677.
Stachyra, T., Guillochon, D., Pulvin, S., and Thomas, D. (1996). “Hemoglobin, horseradish peroxidase, and heme-bovine serum albumin as biocatalyst for the oxidation of dibenzothiophene.” Appl. Biochem. Biotechnol., 59(3), 231–243.
U.S. EPA. (1992). “Method 3650A.” 〈http://legismex.mty.itesm.mx/secc_inter/SW-846/3650a.pdf〉.
U.S. EPA. (1996). “Method 3540C.” 〈http://www.epa.gov/solidwaste/hazard/testmethods/sw846/pdfs/3540c.pdf〉.
Wood, A. W., et al. (1976). “Metabolism of benzo(a)pyrene and benzo (a)pyrene derivatives to mutagenic products by highly purified hepatic microsomal enzymes.” J. Biol. Chem., 251(16), 4882–4890.
Yamada, T., Volkmer, C., and Grisham, M. B. (1991). “The effects of sulfasalazine metabolites on hemoglobin-catalyzed lipid peroxidation.” Free Radic. Biol. Med., 10(1), 41–49.
Information & Authors
Information
Published In
Journal of Environmental Engineering
Volume 141 • Issue 10 • October 2015
Copyright
© 2015 American Society of Civil Engineers.
History
Received: Sep 19, 2014
Accepted: Jan 23, 2015
Published online: Mar 23, 2015
Discussion open until: Aug 23, 2015
Published in print: Oct 1, 2015
ASCE Technical Topics:
- [Inorganic compounds]
- Acids
- Business management
- Carbon
- Chemical compounds
- Chemical degradation
- Chemical elements
- Chemical processes
- Chemicals
- Chemistry
- Energy engineering
- Energy sources (by type)
- Environmental engineering
- Hydrocarbons
- Hydrogen peroxide
- Mitigation and remediation
- Non-renewable energy
- Organic compounds
- Oxidation
- Pollution
- Practice and Profession
- Soil pollution
Authors
Metrics & Citations
Metrics
Citations
Download citation
If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.