Mn(II) Oxidation by Batch Cultures of Pseudomonas putida Strain EC112
Publication: Journal of Environmental Engineering
Volume 141, Issue 3
Abstract
Manganese(II) oxidation by a new bacterium isolate, Pseudomonas putida strain EC112, was studied in batch reactors over a range of Mn(II) concentrations () in a chemically defined liquid medium (at pH 6.5 and 30°C). Bacterial Mn(II) oxidation initiated after the exponential growth phase and carbon source consumption. The best fit kinetic oxidation parameters of half-saturation coefficient and maximum specific substrate utilization rate constant were obtained using Monod-type kinetics and fitted with the Levenberg-Marquardt nonlinear least-squared algorithm. Additional experiments assessed the potential of Mn(II) oxidation by strain EC112 in the presence of the oxidation products of humic acid (HA) and manganese dioxide (). Results show enhanced biological Mn oxidation and growth. This suggests oxidizes biologically refractory organic matter to substrates that support microorganism growth.
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Acknowledgments
This work was supported by funding from Kentucky’s Experimental Program to Stimulate Competitive Research (KY-EPSCoR), Tracy Farmer Environmental Fellowship, Kentucky Water Resources Research Institute (KWRRI) and a teaching assistantship provided by the University of Kentucky, Department of Civil Engineering.
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Journal of Environmental Engineering
Volume 141 • Issue 3 • March 2015
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© 2014 American Society of Civil Engineers.
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Received: Mar 17, 2014
Accepted: Aug 14, 2014
Published online: Sep 22, 2014
Discussion open until: Feb 22, 2015
Published in print: Mar 1, 2015
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