Comparison of Hollow-Fiber Ultrafilters with Pleated Capsule Filters for Surface and Tap Water Samples Using U.S. EPA Method 1623
Publication: Journal of Environmental Engineering
Volume 138, Issue 8
Abstract
The EPA method 1623 is designed specifically for the detection of Cryptosporidium and Giardia, but the method has some issues with low and variable recoveries. Ultrafiltration has been used effectively for microorganism recovery from water samples but is not approved by the EPA. To determine the efficacy of using ultrafiltration, 10-L tap water and surface water samples were seeded with Cryptosporidium and Giardia and concentrated with either a pleated capsule filter or a hollow-fiber ultrafilter. For Cryptosporidum, oocyst recovery in tap water was significantly higher for ultrafiltration (68%) versus the capsule filter (37%); ultrafiltration recovered 65% of oocysts in surface water versus 61% for the capsule filter. However, Giardia cyst recovery was mixed. In tap water, the capsule filter produced a significantly better recovery (85%) of Giardia compared with ultrafiltration (63%), but the surface water ultrafiltration recovery (81%) was significantly better than the capsule filter recovery (40%). Overall, ultrafiltration recoveries were equal to or better for Cryptosporidium, but recoveries of Giardia were varied depending on the filter used and the type of water analyzed.
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Acknowledgments
The use of trade names and names of commercial sources is for identification only and does not imply endorsement by the Centers for Disease Control and Prevention or the U.S. Department of Health and Human Services. The findings and conclusions in this presentation are those of the authors and do not necessarily represent those of the Centers for Disease Control and Prevention.
References
DiGiorgio, C. L., Gonzalez, D. A., and Huitt, C. C. (2002). “Cryptosporidium and Giardia recoveries in natural waters by using environmental protection agency method 1623.” Appl. Environ. Microbiol.AEMIDF, 68(12), 5952–5955.
Hill, V. R., et al. (2005). “Development of a rapid method for simultaneous recovery of diverse microbes in drinking water by ultrafiltration with sodium polyphosphate and surfactants.” Appl. Environ. Microbiol.AEMIDF, 71(11), 6878–6884.
Hill, V. R., Polaczyk, A. L., Kahler, A. M., Cromeans, T. L., Hahn, D., and Amburgey, J. E. (2009). “Comparison of hollow-fiber ultrafiltration to the USEPA VIRADEL technique and USEPA method 1623.” J. Environ. Qual.JEVQAA, 38(2), 822–825.
Hu, J., et al. (2004). “Improvement of recoveries for the determination of protozoa Cryptosporidium and Giardia in water using method 1623.” J. Microbiol. MethodsJMIMDQ, 58(3), 321–325.
MacKenzie, W., et al. (1994). “A massive outbreak in Milwaukee of cryptosporidium infection transmitted through the public water supply.” N. Engl. J. Med.NEJMAG, 331(3), 161–167.
Morales-Morales, H. A., et al. (2003). “Optimization of a reusable hollow-fiber ultrafilter for simultaneous concentration of enteric bacteria, protozoa, and viruses from water.” Appl. Environ. Microbiol.AEMIDF, 69(7), 4098–4102.
Simmons, O. D., Sobsey, M. D., Heaney, C. D., Schaeffer, F. W., and Francy, D. S. (2001). “Concentration and detection of cryptosporidium oocysts in surface water samples by method 1622 using ultrafiltration and capsule method.” Appl. Environ. Microbiol.AEMIDF, 67(3), 1123–1127.
Sobsey, M. D. (1989). “Inactivation of health-related microorganisms in water by disinfection processes.” Water Sci. Technol.WSTED4, 21(3), 179–195.
Solo-Gabrielle, H., and Neumeister, S. (1996). “US outbreaks of cryptosporidiosis.” J. Am. Water Works Assoc.JAWWA5, 88(9), 76–86.
U.S. EPA. (1999). Method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA, Office of Water, Washington, DC.
U.S. EPA. (2006). “National primary drinking water regulations: Long term 2 enhanced surface water treatment rule.” Federal Register, 40 (No. 06-4; Jan. 5), 141–142.
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© 2012. American Society of Civil Engineers.
History
Received: Apr 26, 2011
Accepted: Feb 2, 2012
Published online: Feb 4, 2012
Published in print: Aug 1, 2012
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