Prevalence and Survival of Enterococcus faecium Populations Carrying the esp Gene as a Source-Tracking Marker
Publication: Journal of Environmental Engineering
Volume 137, Issue 5
Abstract
Microbial marking for human fecal pollution with Enterococcus faecium relies on cultivation followed by the detection of the enterococcal surface protein gene (). This study examined the prevalence of the populations carrying the gene among the enterococci populations cultured out of domestic wastewater. Through year-long monitoring at a wastewater treatment plant in Michigan, the proportion of in the enterococci population cultivated from sewage was 0.99%. A more sensitive detection method was developed for disinfected sewage and some impacted ambient waters by using larger membrane filters to increase the volume sampled, and preliminary application of the new protocol showed the presence of in the Grand River, where enterococcal concentrations were low. An strain isolated from wastewater in Michigan was very stable in phosphate buffer and river water at 4 and 25°C, suggesting that it can survive for months after being released into environmental waters. Among 44 samples from human and nonhuman sources, was detected only from samples with human fecal pollution. These results provide evidence that the with large volume sampling is useful as a microbial source tracking marker for disinfected wastewaters.
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Acknowledgments
This study was in part supported by Grant-in-Aid for Young Scientists (Start-up, UNSPECIFIED20860010), Grant-in-Aid for JSPS Fellows (UNSPECIFIED17-04617) from the Japan Society for the Promotion of Science (JSPS), and by a grant from the National Oceanic and Atmospheric Administration (NOAANA04OAR4600199).
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© 2011 American Society of Civil Engineers.
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Received: May 6, 2010
Accepted: Oct 24, 2010
Published online: Apr 15, 2011
Published in print: May 1, 2011
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