Contribution of the Estrogen-Degrading Bacterium Novosphingobium sp. Strain JEM-1 to Estrogen Removal in Wastewater Treatment
Publication: Journal of Environmental Engineering
Volume 136, Issue 9
Abstract
This study aimed to investigate the contribution to estrogen removal from the activated sludge of an estrogen-degrading bacterium, Novosphingobium sp. Strain JEM-1, isolated by the writers from the activated sludge. The cell numbers of the Strain JEM-1 were investigated in two full-scale wastewater-treatment plants using real-time PCR. Strain JEM-1 appears to be commonly distributed in the activated sludge. The cell numbers of Strain JEM-1 in the oxidation ditch process were higher than those in the conventional activated sludge (CAS) process, and the effluent concentrations of E1 in the CAS process tended to decrease with increased cell numbers of Strain JEM-1. In a bench-scale experiment to investigate bioaugmentation with Strain JEM-1, there was a significant difference in the effluent concentrations of estrogens between the experimental series and the control series. Linear relationships were observed between cell numbers of Strain JEM-1 and the efficiency of removal of estrogens. These results suggest that Strain JEM-1 contributes to the estrogen removal in the activated sludge.
Get full access to this article
View all available purchase options and get full access to this article.
References
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D. J. (1997). “Gapped BLAST and PSI-BLAST: A new generation of protein database search programs.” Nucleic Acids Res., 25(17), 3389–3402.
Baronti, C., Curini, R., D’Ascenzo, G., Di Corcia, A., Gentili, A., and Samperi, R. (2000). “Monitoring natural and synthetic estrogens at activated sludge sewage treatment plants and in a receiving river water.” Environ. Sci. Technol., 34(24), 5059–5066.
Carballa, M., Omil, F., Lema, J. M., Llompart, M., García-Jares, C., Rodríguez, I., Gómez, M., and Ternes, T. (2004). “Behavior of pharmaceuticals, cosmetics and hormones in sewage treatment plants.” Water Res., 38(12), 2918–2926.
Clara, M., Kreuzinger, N., Strenn, B., Gans, O., and Kroiss, H. (2005). “The solids retention time—A suitable design parameter to evaluate the capacity of wastewater treatment plants to remove micropollutants.” Water Res., 39(1), 97–106.
Desbrow, C., Routledge, E. J., Brighty, G. C., Sumpter, J. P., and Waldock, M. (1998). “Identification of estrogenic chemicals in STW effluent. 1: Chemical fractionation and in vitro biological screening.” Environ. Sci. Technol., 32(11), 1549–1558.
Fujii, K., Kikuchi, S., Satomi, M., Ushio-Sata, N., and Morita, N. (2002). “Degradation of -estradiol by gram-negative bacterium isolated from activated sludge in a sewage treatment plant in Tokyo, Japan.” Appl. Environ. Microbiol., 68(4), 2057–2060.
Geets, J., de Cooman, M., Wittebolle, L., Heylen, K., Vanparys, B., De Vos, P., Verstraete, W., and Boon, N. (2007). “Real-time PCR assay for the simultaneous quantification of nitrifying and denitrifying bacteria in activated sludge.” Appl. Microbiol. Biotechnol., 75(1), 211–221.
Harms, G., Layton, A. C., Dionisi, H. M., Gregory, I. R., Garrett, V. M., Hawkins, S. A., Robinson, K. G., and Sayler, G. S. (2003). “Real-time PCR quantification of nitrifying bacteria in a municipal wastewater treatment plant.” Environ. Sci. Technol., 37(2), 343–351.
Hashimoto, T., Onda, K., Nakamura, Y., Tada, K., Miya, A., and Murakami, T. (2007). “Comparison of natural estrogen removal efficiency in the conventional activated sludge process and the oxidation ditch process.” Water Res., 41(10), 2117–2126.
Heid, C. A., Stevens, J., Livak, K., and Williams, P. M. (1996). “Real-time quantitative PCR.” Genome Res., 6, 986–994.
Inamori, Y., and Kuniyasu, Y. (1988). “Microbiological experimentation for wastewater treatment.” Experimentation in environmental microbiology, R. Sudo, ed., Kodansha Scientific, Tokyo, 156–159 (in Japanese).
Japan Sewage Works Association. (1997). “Wastewater examination methods.” Tokyo (in Japanese).
Johnson, A. C., Aerni, H. -R., Gerritsen, A., Gibert, M., Giger, W., Hylland, K., Jürgens, M., Nakari, T., Pickering, A., Suter, M. J.-F., Svenson, A., and Wettstein, F. E. (2005). “Comparing steroid estrogen and nonylphenol content across a range of European sewage plants with different treatment and management practices.” Water Res., 39(1), 47–58.
Johnson, A. C., and Sumpter, J. P. (2001). “Removal of endocrine-disrupting chemicals in activated sludge treatment works.” Environ. Sci. Technol., 35(24), 4697–4703.
Khanal, S. M., Xie, B., Thompson, M. L., Sung, S., Ong, S. -K., and Van Leeuwen, J. (2006). “Fate, transport, and biodegradation of natural estrogens in the environment and engineered systems.” Environ. Sci. Technol., 40(21), 6537–6546.
Komori, K., Tanaka, H., Okayasu, Y., Yasojima, M., and Sato, C. (2004). “Analysis and occurrence of estrogens in wastewater in Japan.” Water Sci. Technol., 50(5), 93–100.
Kreuzinger, N., Clara, M., Strenn, B., and Kroiss, H. (2004). “Relevance of the sludge retention time (SRT) as design criteria for wastewater treatment plants for the removal of endocrine disrupters and pharmaceuticals from wastewater.” Water Sci. Technol., 50(5), 149–156.
Onda, K., Morita, T., Luxmy, B. S., Miya, A., Tada, K., Hashimoto, T., and Mishina, F. (2003). “Estrogen-degrading bacteria in activated sludge and their estrogen-degrading characteristics.” Proc., 40th Meeting of Sewage Works, Japan Sewage Works Association, Tokyo, 90–92 (in Japanese).
Routledge, E. J., Sheahan, D. A., Desbrow, C., Brighty, G. C., Waldock, M., and Sumpter, J. P. (1998). “Identification of estrogenic chemicals in STW effluent. 2: In vivo response in trout and roach.” Environ. Sci. Technol., 32(11), 1559–1565.
Routledge, E. J., and Sumpter, J. P. (1996). “Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen.” Environ. Toxicol. Chem., 15(3), 241–248.
Servos, M. R., Bennie, D. T., Burnison, B. K., Jurkovic, A., McInnis, R., Neheli, T., Schnell, A., Seto, P., Smyth, S. A., and Ternes, T. A. (2005). “Distribution of estrogens, -estradiol and estrone, in Canadian municipal wastewater treatment plants.” Sci. Total Environ., 336(1–3), 155–170.
Snyder, S. A., Villeneuve, D. L., Snyder, E. M., and Giesy, J. P. (2001). “Identification and quantification of estrogen receptor agonists in wastewater effluents.” Environ. Sci. Technol., 35(18), 3620–3625.
Tanaka, H., Yakou, Y., Takahashi, A., Higashitani, T. and Komori, K. (2001). “Comparison between estrogenicities estimated from DNA recombinant yeast assay and from chemical analyses of endocrine disrupters during sewage treatment.” Water Sci. Technol., 43(2), 125–132.
Vervaeren, H., De Wilde, K., Matthys, J., Boon, N., Raskin, L., and Verstraete, W. (2005). “Quantification of an Eikelboom type 021N bulking event with fluorescence in situ hybridization and real-time PCR.” Appl. Microbiol. Biotechnol., 68(5), 695–704.
Yu, C. -P., Roh, H., and Chu, K. -H. (2007). “ -estradiol-degrading bacteria isolated from activated sludge.” Environ. Sci. Technol., 41, 486–492.
Zhang, T., and Fang, H. H. P. (2006). “Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples.” Appl. Microbiol. Biotechnol., 70(3), 281–289.
Zhu, H., Qu, F., and Zhu, L. -H. (1993). “Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride.” Nucleic Acids Res., 21(22), 5279–5280.
Information & Authors
Information
Published In
Journal of Environmental Engineering
Volume 136 • Issue 9 • September 2010
Pages: 890 - 896
Copyright
© 2010 ASCE.
History
Received: Apr 14, 2008
Accepted: Dec 28, 2009
Published online: Dec 30, 2009
Published in print: Sep 2010
Authors
Metrics & Citations
Metrics
Citations
Download citation
If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.