TECHNICAL PAPERS
Feb 1, 2008

Impact of Initial pH and Linoleic Acid (C18:2) on Hydrogen Production by a Mesophilic Anaerobic Mixed Culture

Publication: Journal of Environmental Engineering
Volume 134, Issue 2

Abstract

Hydrogen production from glucose using a mixed anaerobic culture was assessed under batch conditions by adjusting the initial pH and adding linoleic acid (LA). At an initial pH of 5.0, hydrogen ( 1.9molH2mol1 glucose) was detected after the first glucose injection in the controls (no LA added). At the latter initial pH and in cultures fed 2,000mgl1 LA, the yield reached a maximum of 2.4molH2mol1 glucose when glucose was injected again. Hydrogen was detected after the second glucose injection in cultures with the initial pH adjusted to 5.0 and 6.0. In cultures receiving LA and adjusted to pH 7.6, the hydrogen yield reached 1.4molH2mol1 glucose when glucose was injected again. Acetate, propionate, and butyrate were detected under all conditions; however, the quantities were variable and dependent on the conditions examined. In comparison to the amount of volatile fatty acids produced, relatively low quantities of alcohols (ethanol, i-propanol, n-butanol, and i-butanol) were detected during the initial lag phase of 96to120h .

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Acknowledgments

Financial support for this work was provided by the Natural Sciences and Engineering Research Council of Canada (NSERC)NSERC and the University of Windsor.

References

Benemann, J. R. (2000). “Hydrogen production by microalgae.” J. Appl. Phycol., 12(3–5), 291–300.
Casper, M. S. (1978). Hydrogen manufacture by electrolysis, thermal decomposition and unusual techniques, Noyes Data Corp., Park Ridge, N.J.
Chen, C.-C., Lin, C.-Y., and Lin, M.-C. (2002). “Acid-base enrichment enhances anaerobic hydrogen production process.” Appl. Microbiol. Biotechnol., 58(2), 224–228.
Dabrock, B., Bahl, H., and Gottschalk, H. (1992). “Parameters affecting solvent production by Clostridium pasteurianum.” Appl. Environ. Microbiol., 58(4), 1233–1239.
Dürre, P. (1998). “New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation.” Appl. Microbiol. Biotechnol., 49(6), 639–648.
Dürre, P. (2005). “Formation of solvents in Clostridia.” Handbook on Clostridia, P. Dürre, ed., Taylor and Francis, New York, 671–693.
Fang, H. H. P., Liu, H., and Zhang, T. (2004). “Biohydrogen production from wastewater.” Water Sci. Technol., 4(1), 77–86.
Hallenbeck, P. C., and Benemann, J. R. (2002). “Biological hydrogen production: Fundamentals and limiting processes.” Int. J. Hydrogen Energy, 27(11–12), 1185–1193.
Han, S.-K., and Shin, H.-S. (2004). “Biohydrogen production by anaerobic fermentation of food waste.” Int. J. Hydrogen Energy, 29(6), 569–577.
Hansen, J., et al. (1981). “Climate impact of increasing atmospheric carbon dioxide.” Science, 213(4511), 957–966.
Hawkes, F. R., Dinsdale, R. D., Hawkes, D. L., and Hussy, I. (2002). “Sustainable fermentative hydrogen production: Challenges for process optimization.” Int. J. Hydrogen Energy, 27(11–12), 1339–1347.
Holt, R. A., Stephens, G. M., and Morris, J. G. (1984). “Production of solvents by Clostridium acetobutylicum cultures maintained at neutral pH.” Appl. Environ. Microbiol., 48(6), 1166–1170.
Huang, J.-S., Jih, C.-G., Lin, S.-D., and Ting, W.-H. (2003). “Process kinetics of UASB reactors.” J. Chem. Technol. Biotechnol., 78(7), 762–772.
Hwu, C. S., and Lettinga, G. (1997). “Acute toxicity of oleate to acetate-utilizing methanogens in mesophilic and thermophilic anaerobic sludges.” Enzyme Microb. Technol., 21(4), 297–301.
Islam, R., Cicek, N., Sparling, R., and Levin, D. (2006). “Effect of substrate loading on hydrogen production during anaerobic fermentation by Clostridium thermocellum 27405.” Appl. Microbiol. Biotechnol., 72, 576–583.
Jones, D. T., and Woods, D. R. (1986). “Acetone-butanol fermentation revisited.” Microbiol. Rev., 50(4), 484–524.
Jones, D. T., and Woods, D. R. (1989). “Solvent production.” Clostridia biotechnology handbook, N. P. Minton and D. J. Clarke, eds., Vol. 3, Plenum Press, New York, 105–144.
Kargi, F., and Kapdan, I. K. (2005). “Biohydrogen production from waste materials.” Proc., Int. Hydrogen Energy Congress and Exhibition, Istanbul, Turkey, 1–12.
Khanal, S. K., Chen, W. H., Li, L., and Sung, S. (2004). “Biological hydrogen production: Effects of pH and intermediate products.” Int. J. Hydrogen Energy, 29(11), 1123–1131.
Khanal, S. K., Chen, W. H., Li, L., and Sung, S. (2006). “Biohydrogen production in continuous-flow reactor using mixed microbial culture.” Water Environ. Res., 78(2), 110–117.
Kim, S., Hwanga, M. H., Jang, N. J., Hyun, S. H., and Lee, S. T. (2004). “Effect of low pH on the activity of hydrogen utilizing methanogen in bio-hydrogen process.” Int. J. Hydrogen Energy, 29(11), 1133–1140.
Kumar, N., and Das, D. (2000). “Enhancement of hydrogen production by Enterobacter cloacae IIT-BT.” Process Biochem. (Oxford, U.K.), 35(6), 589–593.
Lalman, J. A., Alosta, H., Bejankiwar, R., and Bellmer, D. (2003). “Kinetics of glucose fermentation by a mixed culture in the presence of linoleic, oleic, and stearic acid.” Environ. Technol., 24(12), 1471–1478.
Lalman, J. A., and Bagley, D. M. (2000). “Anaerobic degradation and inhibitory effects of linoleic acid.” Water Res., 34(17), 4220–4228.
Lalman, J. A., and Bagley, D. M. (2002). “Effects of C18 long chain fatty acids on glucose, butyrate and hydrogen degradation.” Water Res., 36(13), 3307–3313.
Lee, Y. J., Miyahara, T., and Noike, T. (2002). “Effect of pH on microbial hydrogen fermentation.” J. Chem. Technol. Biotechnol., 77(6), 694–698.
Lide, D. R. (2006). “Heat of combustion.” CRC handbook of chemistry and physics, 87th Ed., Taylor and Francis, Boca Raton, Fla., 5–70.
Liu, H., and Fang, H. H. P. (2002). “Effect of pH on hydrogen production from glucose by a mixed culture.” Bioresour. Technol., 82(2), 87–93.
Lodhi, M. A. K. (1987). “Hydrogen production from renewable sources of energy.” Int. J. Hydrogen Energy, 12(7), 461–468.
Miyake, J., Miyake, M., and Asada, Y. (1999). “Biotechnological hydrogen production: Research for efficient light energy conversion.” J. Biotechnol., 70(1), 89–101.
Mykhaylovin, O., Roy, J. M., Jing, N., and Lalman, J. A. (2005). “Influence of C18 long chain fatty acids on butyrate degradation by a mixed culture.” J. Chem. Technol. Biotechnol., 80(2), 169–175.
Nandi, R., and Sengupta, S. (1998). “Microbial production of hydrogen: An overview.” Crit. Rev. Microbiol., 24(1), 61–84.
Nath, K., and Das, D. (2004). “Improvement of fermentative hydrogen production: Various approaches.” Appl. Microbiol. Biotechnol., 65(5), 520–529.
National Research Council (NRC) and National Academy of Engineering (NAE). (2004). “The hydrogen economy: Opportunities, costs, barriers, and R&D needs.” Hydrogen production technologies, National Academic Press, Washington, D.C., 91–105.
Novak, J. T., and Carlson, D. A. (1970). “The kinetics of anaerobic long chain fatty acids degradation.” J. Water Pollut. Control Fed., 42(11), 1932–1943.
Oh, S.-E., and Logan, B. E. (2005). “Hydrogen and electricity production from a food processing wastewater using fermentation and microbial fuel cell technologies.” Water Res., 39(19), 4673–4682.
Oh, S.-E., Van Ginkel, S., and Logan, B. E. (2003). “The relative effectiveness of pH control and heat treatment for enhancing biohydrogen gas production.” Environ. Sci. Technol., 37(22), 5186–5190.
Ough, C. S., Guymon, J. F., and Crowell, E. A. (1966). “Formation of higher alcohols during grape juice fermentations at various temperatures.” J. Food. Sci., 31(4), 620–625.
Park, S. J., et al. (1996). “Propionic acid accumulation and controlling factors in anaerobic treatment of carbohydrate: Effects of H2 and pH.” Water Sci. Technol., 34(5–6), 317–325.
Rinzema, A., Boone, M., van Knippenberg, K., and Lettinga, G. (1994). “Bactericidal effect of long chain fatty acids in anaerobic digestion.” Water Environ. Res., 66(1), 40–49.
Rosen, M. A., and Scott, D. S. (1998). “Comparative efficiency assessments for a range of hydrogen production processes.” Int. J. Hydrogen Energy, 23(8), 653–659.
Sparling, R., Risbey, D., and Poggi-Varaldo, H. M. (1997). “Hydrogen production from inhibited anaerobic composters.” Int. J. Hydrogen Energy, 22(6), 563–566.
Sung, S., Raskin, L., Duangmanee, T., Padmasiri, S., and Simmons, J. J. (2002). “Hydrogen production by anaerobic microbial communities exposed to repeated heat treatments.” Proc., 2002 U.S. DOE Hydrogen Program Rev., Golden, Colo., 1–17, Paper No. NREL/CP-610–32405.
Templer, J., Lalman, J. A., and Ndegwa, P. M. (2006). “Influence of C18 long chain fatty acids on hydrogen metabolism.” Biotechnol. Prog., 22(1), 199–207.
Terracciano, J. S., and Kashket, E. R. (1986). “Intracellular conditions required for initiation of solvent production by Clostridium acetobutylicum.” Appl. Environ. Microbiol., 52(1), 86–91.
Thauer, R. K., Jungermann, K., and Decker, K. (1977). “Energy conservation in chemotrophic anaerobic bacteria.” Bacteriol. Rev., 41(1), 100–180.
Trimm, D. L., and Önsan, Z. İ. (2001). “On board fuel conversion for hydrogen fuel cell driven vehicles.” Catal. Rev. - Sci. Eng., 43(1–2); 31–84.
Van Ginkel, S., and Logan, B. E. (2005). “Inhibition of biohydrogen production by undissociated acetic and butyric acids.” Environ. Sci. Technol., 39(23), 9351–9356.
Van Ginkel, S. V., Sung, S., and Lay, J. J. (2001). “Biohydrogen production as a function of pH and substrate concentration.” Environ. Sci. Technol., 35(24), 4726–4730.
Weigant, W. M., and Lettinga, G. (1985). “Thermophilic anaerobic digestion of sugars in upflow anaerobic sludge blanket reactors.” Biotechnol. Bioeng., 27(11), 1603–1607.
Weng, C. N., and Jeris, J. S. (1976). “Biochemical mechanisms in the methane fermentation of glutamic and oleic acids.” Water Res., 10(1), 9–18.
Zheng, X. J., and Yu, H. Q. (2004). “Roles of pH in biologic production of hydrogen and volatile fatty acids from glucose by enriched anaerobic cultures.” Appl. Biochem. Biotechnol., 112(2), 79–90.

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Published In

Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 134Issue 2February 2008
Pages: 110 - 117

History

Received: Nov 27, 2006
Accepted: Jul 27, 2007
Published online: Feb 1, 2008
Published in print: Feb 2008

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Authors

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Srimanta Ray [email protected]
Graduate Student, Dept. of Civil and Environmental Engineering, Univ. of Windsor, 335 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4. E-mail: [email protected]
Nabin Chowdhury [email protected]
Graduate Student, Dept. of Civil and Environmental Engineering, Univ. of Western Ontario, London, Ontario, Canada N6A 5B9. E-mail: [email protected]
Jerald A. Lalman [email protected]
Associate Professor, Dept. of Civil and Environmental Engineering, Univ. of Windsor, 334 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4 (corresponding author). E-mail: [email protected]
Rajesh Seth [email protected]
Assistant Professor, Dept. of Civil and Environmental Engineering, Univ. of Windsor, B07 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4. E-mail: [email protected]
Nihar Biswas [email protected]
Professor, Dept. of Civil and Environmental Engineering, Univ. of Windsor, 147 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4. E-mail: [email protected]

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