Biohydrogen Production by Mesophilic Anaerobic Fermentation of Glucose in the Presence of Linoleic Acid
Publication: Journal of Environmental Engineering
Volume 133, Issue 12
Abstract
In this work, linoleic acid (LA), a long chain fatty acid bearing 18 carbons and two double bonds, inhibited hydrogen consumption in a mixed anaerobic culture acclimated to glucose. At , a metabolic shift from methane to hydrogen formation was observed in cultures maintained at and fed glucose in the presence of LA. The hydrogen yield increased with increasing LA levels while the quantity of methane decreased. The major volatile fatty acids produced were acetate and butyrate with greater levels observed in cultures fed with LA. Acetate, butyrate, and hydrogen accumulation suggests inhibition of aceticlastic methanogens, hydrogenotrophic methanogens, and butyrate degrading microorganisms, respectively, in the presence of LA. A maximum hydrogen yield of glucose was observed only when glucose was reinjected into cultures receiving LA plus glucose.
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Acknowledgments
Funding to support this work was provided by Discovery Grants from the Natural Sciences and Engineering Research Council of CanadaNRC (NSERC) and graduate assistantships from the University of Windsor.
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Information
Published In
Journal of Environmental Engineering
Volume 133 • Issue 12 • December 2007
Pages: 1145 - 1152
Copyright
© 2007 ASCE.
History
Received: Jul 5, 2006
Accepted: Apr 19, 2007
Published online: Dec 1, 2007
Published in print: Dec 2007
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