Effect of Ozone on Filamentous Bulking in a Laboratory Scale Activated Sludge Reactor Using Respirometry and INT-Dehydrogenase Activity
Publication: Journal of Environmental Engineering
Volume 132, Issue 9
Abstract
Filamentous bulking can be controlled by the addition of oxidant chemical agents such as ozone. To evaluate the ozone effect on activated sludge from a laboratory scale reactor, different techniques were applied: Settleability test, respirometry (oxygen uptake rate), and the INT-dehydrogenase activity test carried out both by spectrophotometry and image analysis . In activated sludge, the respirometric technique and the spectrophotometric quantified ozone action on the total respiratory activity of flocs; in contrast, the image test was applied to evaluate the specific action of ozone on filamentous microorganisms. The conditions for application of the INT-dehydrogenase activity test were standardized using pure cultures of a filamentous microorganism (Sphaerotilus natans) and a floc-forming bacterium (Acinetobacter anitratus). For activated sludge with filamentous bulking, ozone doses and treatment times necessary to improve settleability were established. Ozone dose levels and contact times influence the viability of bacteria in flocs and filaments and the finding of appropriate parameters to preserve floc viability and, at the same time, inhibiting filaments is essential to filament control by ozonation.
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Acknowledgments
The writers gratefully acknowledge the financial support given by the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), the Universidad Nacional de La Plata, and the Agencia Nacional de Promoción Científica y Tecnológica, Argentina.
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Information
Published In
Journal of Environmental Engineering
Volume 132 • Issue 9 • September 2006
Pages: 1001 - 1010
Copyright
© 2006 ASCE.
History
Received: Mar 23, 2005
Accepted: Dec 5, 2005
Published online: Sep 1, 2006
Published in print: Sep 2006
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