Arsenite Oxidation by Alcaligenes faecalis Strain O1201
Publication: Journal of Environmental Engineering
Volume 131, Issue 9
Abstract
Batch experiments were conducted to examine the oxidation of arsenite (As (III)) to arsenate (As (V)) by Alcaligenes faecalis strain O1201 isolated from soils. Pure cultures of O1201 completely oxidized As (III) to As (V) in the exponential growth phase at As (III) concentrations ranging from 10 to in less than 12 h. The growth of O1201 requires an organic substrate as the carbon and energy source, and the oxidation of As (III) was mainly observed in the exponential growth phase. As (III) concentration at inhibited the growth of O1201, whereas its oxidation product As (V) did not show any inhibition effects even at concentration as high as . Kinetics studies of As (III) oxidation were performed under the optimal conditions for O1201 at pH 7 and 30°C in a chemical defined medium with citrate as the sole carbon source. The Monod expression coupled with a logistic growth model was used to analyze the kinetics of As (III) oxidation. The best fit parameters of half-velocity coefficient of , maximum reaction rate coefficient of 0.47 mg As dry weight/h, and logistic growth rate coefficient of were obtained using a nonlinear regression analysis.
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Acknowledgments
This work was supported by the Kentucky Science & Engineering Foundation through a R & D Excellence grant awarded to Yi-Tin Wang under Agreement No. KSEFKSEF-148-502-03-71. Additional support was provided by the Raymond Civil Engineering Graduate Fellowship of the University of Kentucky. The writers are grateful to Craig A. Cox for providing soil samples and Suwit Wuthisuthimethavee for performing the partial sequencing of the 16S rDNA gene.
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Received: Feb 24, 2004
Accepted: Feb 3, 2005
Published online: Sep 1, 2005
Published in print: Sep 2005
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