TECHNICAL PAPERS
Apr 1, 2005

Factors Affecting Hydrogen Production from Food Wastes by Clostridium-Rich Composts

Publication: Journal of Environmental Engineering
Volume 131, Issue 4

Abstract

This study used the technique of response surface approach to analyze the combined effects of heat-shocking temperature and time on anaerobic grass composts. Results indicate that the grass composts under heat-shocking temperature and time of 80°C and 3h , respectively, could yield high populations of hydrogen-producing microorganisms. Metabolic results demonstrate that the composts are reliable, having considerable hydrogen-producing Clostridia. The multivariate analysis with response surface by considering specific hydrogen-producing potential and rate simultaneously indicate that the cultural media with Fe2+=132mgL ; NH4+=537mgL ; and PO43=1,331mgL were optimal for the hydrogen-producing Clostridia-rich composts using high-solids food wastes. The specific hydrogen production potential and rate were 77±3mLH2gTVS and 520±20mLH2gTVSday , respectively. The former was 38% of theoretical hydrogen-producing potential of Clostridium sp. using glucose. Of these factors, ammonium and phosphate are nutrients for the hydrogen-producing Clostridia growth while iron exerts a synergistic influence on them in the conversion of the food wastes into hydrogen.

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Acknowledgment

The writers thank the Bureau of Energy Commissions, Ministry of Economic Affairs of the Republic of China, for financial support of this work.

References

American Public Health Association/American Water Works Association/Water Pollution Control Federation (APHA/AWA/WPCF). (1995). Standard methods for the examination of water and wastewater, 19th Ed., APHA, Washington, D.C.
Bahl, H., Gottwald, M., Kuhn, A., Rale, V., Andersch, W., and Gottschalk, G. (1986). “Nutritional factors affecting the ratio of solvents produced by Clostridium acetobutylicum.” Appl. Environ. Microbiol., 52, 169–172.
Box, G. E. P., Hunter, W. G., and Hunter, J. S. (1978). Statistics for experimenters, Wiley, New York.
Brosseau, J. D., Yan, J.-Y., and Lo, K. V. (1986). “The relationship between hydrogen gas and butanol production by Clostridium saccharoperbutylacetonicum.” Biotechnol. Bioeng., 28, 305–310.
Dabrock, B., Bahl, H., and Gottschalk, G. (1992). “Parameters affecting solvent production by Clostridium pasteurianum.” Appl. Environ. Microbiol., 58(4), 1233–1239.
Giallo, J., Gaudin, C., and Belaich, J.-P. (1985). “Metabolism and solubilization of cellulose by Clostridium cellulolyticum H10.” Appl. Environ. Microbiol., 49(5), 1216–1221.
Gray, C. T., and Gest, H. (1965). “Biological formation of molecular hydrogen.” Science, 148, 186–191.
Han, K., and Levenspiel, O. (1988). “Extended Monod kinetics for substrate, product, and cell inhibition.” Biotechnol. Bioeng., 32, 430–437.
Jones, D. T., and Woods, D. R. (1986). “Acetone-butanol fermentation revisited.” Microbiol. Rev., 50, 484–524.
Kim, B. H., Bellows, P., Datta, R., and Zeikus, J. G. (1984). “Control of carbon and electron flow in Clostridium acetobutylicum fermentation: Utilization of carbon monoxide to inhibit hydrogen production and to enhance butanol yield.” Appl. Environ. Microbiol., 43, 1434–1439.
Lay, J. J. (2000). “Modeling and optimization of anaerobic digested sludge converting starch to hydrogen.” Biotechnol. Bioeng., 68, 269–278.
Lay, J. J. (2001). “Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose.” Biotechnol. Bioeng., 74(4), 280–287.
Lay, J. J., Fan, K. S., Chang, J. I., and Ku, C. H. (2003). “Influence of chemical nature of organic wastes on their conversion to hydrogen by heat-shock digested sludge.” Int. J. Hydrogen Energy, 28, 1361–1367.
Lay, J. J., Lee, Y. J., and Noike, T. (1999). “Feasibility of biological hydrogen production from organic fraction of municipal solid waste.” Water Res., 33(11), 2579–2586.
Mitchell, W. J. (1998). “Physiology of carbohydrate to solvent conversion by Clostridia.” Adv. Microb. Physiol., 39, 31–130.
Morvan, B., Rieu-Lesme, F., Fonty, G., and Gouet, P. (1996). “In vitro interactions between rumen H2 -producing cellulolytic microorganisms and H2 -utilizing acetogenic and sulfate reducing bacteria.” Anaerobe, 2, 175–180.
Mosey, F. E., and Hughes, D. A. (1975). “Toxicity of heavy metal ions to anaerobic digestion.” Water Pollut. Control, 74, 18–39.
Owen, W. F., Stuckey, D. C., Healy, J. B., Jr., Young, L. Y., and McCarty, P. L. (1979). “Bioassay for monitoring biochemical methane potential and anaerobic toxicity.” Water Res., 13, 485–493.
Roychowdhury, S., Cox, D., and Levandowsky, M. (1988). “Production of hydrogen by microbial fermentation.” Int. J. Hydrogen Energy, 13(7), 407–410.
Sparling, R., Risbey, D., and Poggi-Varaldo, H. M. (1997). “Hydrogen production from inhibited anaerobic composters.” Int. J. Hydrogen Energy, 22(6), 563–566.
Van Ginkel, S., Sung, S., and Lay, J. J. (2001). “Biohydrogen production as a function of pH and substrate concentration.” Environ. Sci. Technol., 35, 4726–4730.

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Published In

Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 131Issue 4April 2005
Pages: 595 - 602

History

Received: Jan 21, 2003
Accepted: May 17, 2004
Published online: Apr 1, 2005
Published in print: Apr 2005

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Authors

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Jiunn-Jyi Lay [email protected]
Energy Research Centre, Dept. of Safety, Health and Environmental Engineering, National Kaohsiung First Univ. of Science and Technology, 1 Univ. Rd., Yanchau, Kaohsiung, Taiwan, R.O.C. E-mail: [email protected]
Kuo-Shuh Fan
Dept. of Safety, Health and Environmental Engineering, National Kaohsiung First Univ. of Science and Technology, 1 Univ. Rd., Yanchau, Kaohsiung, Taiwan, R.O.C.
Jieng-I Hwang
Dept. of Safety, Health and Environmental Engineering, National Kaohsiung First Univ. of Science and Technology, 1 Univ. Rd., Yanchau, Kaohsiung, Taiwan, R.O.C.
James-I Chang
Dept. of Safety, Health and Environmental Engineering, National Kaohsiung First Univ. of Science and Technology, 1 Univ. Rd., Yanchau, Kaohsiung, Taiwan, R.O.C.
Ping-Chi Hsu
Dept. of Safety, Health and Environmental Engineering, National Kaohsiung First Univ. of Science and Technology, 1 Univ. Rd., Yanchau, Kaohsiung, Taiwan, R.O.C.

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