Effect of Nonsteady state Tests on Oxygen Transfer Rate in Enhanced Biological Phosphorus Removal Process
Publication: Journal of Environmental Engineering
Volume 131, Issue 12
Abstract
A laboratory scale enhanced biological phosphorus removal process was operated in the University of Cape Town configuration to study the variations in alpha and oxygen transfer efficiency under different process conditions. As part of this investigation, process oxygen transfer parameters were determined using the steady state oxygen uptake rate (OUR) and the nonsteady state hydrogen peroxide addition (HPA) methods, as per the American Society of Civil Engineers guidelines. The results indicated that the oxygen transfer parameters [volumetric mass transfer coefficient , oxygen transfer rate , and )] were higher when both methods were applied on the same day, compared to the subsequent period, when only the steady state OUR method was employed, under similar operating conditions. The difference in the oxygen transfer parameters appears to be due to the addition of that generates reactive oxygen species in the nonsteady state HPA test. Based on the findings, it was concluded that the HPA test was not a suitable technique to measure oxygen transfer under process conditions. Further, a conceptual model hypothesizing the impacts of addition on activated sludge process is presented.
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Acknowledgments
This work was financially supported by the Natural Sciences and Engineering Research Council (NSERC) of Canada. The writers also wish to acknowledge the excellent technical backup provided by Susan Harper and Paula Parkinson of the UBC Environmental Engineering Laboratory. This research was conducted at the University of British Columbia as part of the doctoral dissertation of the first writer (V.M.).
References
American Public Health Association, American Water Works Association, and Water Environment Federation (APHA/AWWA/WEF). (1992). Standard methods for the examination of water and wastewater, 18th Ed., APHA, Washington, D.C.
American Society of Civil Engineers (ASCE). (1992). “A standard for the measurement of oxygen transfer in clean water.” Standards No. 88-001, ASCE Oxygen Standards Transfer Committee, ASCE, New York.
American Society of Civil Engineers (ASCE). (1997). “Standard guidelines for in-oxygen transfer testing.” Standards No. 96-018, ASCE Oxygen Transfer Standards Subcommittee, ASCE, New York.
Baburin, L. A., Shvinka, J. E., and Viesturs, U. E. (1981). “Equilibrium oxygen concentration in fermentation fluids.” Eur. J. Appl. Microbiol. Biotechnol., 13, 15–18.
Beckman, K. B., and Ames, B. N. (1998). ”The free radical theory of aging matures.” Psychol. Rev., 78(2), 547–581.
Cabiscol, E., Tamarit, J., and Ros, J. (2000). “Oxidative stress in bacteria and protein damage by reactive oxygen species.” Int. Microbiol., 3, 3–8.
Cannio, R., Fiorentino, G., Morana, A., Rossi, M., and Bartolucci, A. N. (2000). “Oxygen: Friend or foe? Archaeal superoxide dismutases in of intra- and extracelluar oxidative stress.” Front. Biosci., 5, d768–779.
Chamarro, E., Marco, A., and Esplugas, S. (2001). “Use of Fenton reagent to improve organic chemical biodegradability.” Water Res., 35(4), 1047–1051.
Farr, S. B., and Kogoma, T. (1991). “Oxidative stress responses in Escherichia coli and Salmonella typhimurium.” Microbiol. Rev., 55, 561–583.
Flecha, B. G., and Demple, B. (1997). “Homeostatic regulation of intracellular hydrogen peroxide concentration in aerobically grown Escherichia coli.” J. Bacteriol., 179(2), 382–388.
Flecha, B. G., and Demple, B. (1995). “Metabolic sources of hydrogen peroxide in aerobically growing Escherichia coli.” J. Biochem. (Tokyo), 270(23), 13681–13687.
Flecha, B. G., and Demple, B. (1999). ”Role of oxyR gene in regulation of intracellular hydrogen peroxide in Escherichia coli.” J. Bacteriol., 181(12), 3833–3836.
Fridovich, I. (1978). “The biology of oxygen radicals.” Science, 201, 875–880.
Galhardo, R. S., Almeida, C. E. B., Leitao, A. C., and Cabral-neto, J. B. (2000). “Repair of DNA lesions induced by hydrogen peroxide in the presence of iron chelators in Escherichia coli: Participation of endonuclease IV and Fpg.” J. Bacteriol., 182(7), 1964–1968.
Gates-Anderson, D. D., Siegrist, R. L., and Cline, S. R. (2001). “Comparison of potassium permanganate and hydrogen peroxide as chemical oxidants for organically contaminated soils.” J. Environ. Eng., 127(4), 337–347.
Gerschman, K., Gilbert, L., Nye, S. W., Dwyer, P., and Fenn, W. O. (1954). “Oxygen poisoning and X-irradiation: Amechanism in common.” Science, 11, 623–626.
Grady, C. P. L., Jr., Daigger, G. T., and Lim, H. C. (1999). Biological wastewater treatment, 2nd Ed., Marcel Dekker, New York.
Halliwell, B. (1984). “Oxygen radicals: A commonsense look at their nature and medical importance.” Med. Biol., 62, 71–77.
Harper, W. F., Jr. (2003). “The effect of an initial anaerobic zone on the kinetics and stoichiometry of acetate removal during nutrient-limiting conditions.” Water SA, 29(4), 443–450.
Houtmeyers, J., Poffe, R., and Verachtert, H. (1977). “Hydrogen peroxide as a supplemental oxygen for activated sludge: Microbiological investigations.” Eur. J. Appl. Microbiol., 4, 295–305.
Imlay, J. A., and Linn, S. (1988). “DNA damage and oxygen radical toxicity.” Science, 240, 1302–1309.
Jakubovics, N. S., and Jenkinson, H. F. (2001). ”Out of the iron age: New insights into the critical role of manganese homeostasis in bacteria.” Microbiology, 147, 1709–1718.
Jormakka, M., Byrne, B., and Iwata, S. (2003). “Proton motive force generation by redox loop mechanism.” FEBS Lett., 545, 25–30.
Kayser, R. (1979). “Measurements of oxygen transfer in clean and process water conditions.” Prog. Water Technol., 11(3), 23–36.
Konz, J. O., King, J., and Cooney, C. L. (1998). “Effects of oxygen on recombinant protein expression.” Biotechnol. Prog., 14, 393–409.
Lee, A., Ma, J., and Stenstrom, M. K. (2000). “ASCE DO parameter estimation program (DO PAR).” Dept. of Civil and Environmental Engineering, Univ. of California at Los Angeles, Los Angeles, ⟨http://fields.seas.ucla.edu/research/dopar⟩ accessed December 10, 2000.
Lin, S. S., and Gurol, M. D. (1998). “Catalytic decomposition of hydrogen peroxide on iron oxide: Kinetics, mechanism, and implications.” Environ. Sci. Technol., 32, 1417–1423.
Madigan, M. T., Martinko, J. M., and Parker, J. (1997). Brock-biology of microorganisms, 8th Ed., Prentice-Hall, Englewood Cliffs, N.J.
Mahendraker, V. (2003). ”Development of a unified theory of oxygen transfer in activated sludge processes—the concept of net respiration rate flux.” PhD thesis, Environmental Engineering Program, Dept. of Civil Engineering, Univ. of British Columbia, Vancouver, B.C., Canada.
Mahendraker, V., Mavinic, D. S., and Rabinowitz, B. (2002). “Impact of hydrogen peroxide oxygen transfer tests on the performance of the biological nutrient removal process.” Environ. Technol., 23, 149–162.
Neidhart, F. C., Ingraham, J. L., and Schaechter, M. (1990). Physiology of the bacterial cell—a molecular approach, Sinauer Associates Inc., Mass.
Riondet, C., Cachon, R., Wache, Y., Alcaraz, G., and Divies, C. (1999). “Changes in proton-motive force in Escherichia coli in response to external oxidation-reduction potential.” Eur. J. Biochem. 262, 695–599.
Russell, J. B., and Cook, G. M. (1995). “Energetics of bacterial cell growth: Balance of anabolic and catabolic reactions.” Microbiol. Rev., 59(1), 48–62.
Schafer, F. Q., and Buettner, G. R. (2001). ”Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple.” Free Radic Biol. Med., 30(11), 1191–1212.
SPSS Inc. (1999). SYSTAT, 9th Ed., Chicago, ⟨http:/www.spss.com⟩.
Tran, Q. H., and Unden, G. (1998). “Changes in proton potential and the cellular energetics of Escherichia coli during growth by aerobic and anaerobic respiration or fermentation.” Eur. J. Biochem., 251, 538–543.
United States Environmental Protection Agency (USEPA). (1989a). “Design manual—fine pore aeration systems.” EPA/625/1-89/023, USEPA, Washington, D.C.
United States Environmental Protection Agency (USEPA). (1989b). “Summary report: Fine pore (fine bubble) aeration systems.” EPA-625-8-85-010, USEPA, Washington, D.C.
Waddel, J. P., and Mayer, G. C. (2003). ”Effects of Fenton reagent and potassium permanganate applications on indigenous subsurface microbiota: A literature review.” Proc., 2003 Georgia Water Resources Conf., University of Georgia, Athens, Ga.
Walling, C. W. (1975). Fenton’s reagent revisited. Acc. Chem. Res., 8, 125–131.
Wiseman, H., and Halliwell, B. (1996). “Damage to DNA by reactive oxygen and nitrogen species: Role in inflammatory disease and progression of cancer.” Biochem. J. 313, 17–29.
Wojtaszek, P. (1997). ”Oxidative burst: An early plant response to pathogen infection.” Biochem. J. 322, 681–692.
Wu, Y., Mundy, C. J., Colvin, M. E., and Car, R. (2004). ”On the mechanism of OH radical induced DNA-base damage: A comparative quantum chemical and Car-Parrinello molecular dynamics study.” J. Phys. Chem., 108, 2922–2929.
Xu, S., and Hasselblad, S. (1996). “A simple biological method to estimate the readily biodegradable organic matter in wastewater.” Water Res., 30(4), 1023–1025.
Zhang, Y., Marcillat, O., Giulivi, C., Ernster, L., and Davies, K. J. A. (1990). “The oxidative inactivation of mitochondrial electron transport chain components and ATPase.” J. Biol. Chem., 265(27), 16330–16336.
Zilberstein, D., Agmon, V., Schuldiner, S., and Padn, E. (1984). “Escherichia coli intracellular pH, membrane potential, and cell growth.” J. Bacteriol., 158(1), 246–252.
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Information
Published In
Journal of Environmental Engineering
Volume 131 • Issue 12 • December 2005
Pages: 1684 - 1697
Copyright
© 2005 ASCE.
History
Received: Sep 5, 2003
Accepted: Feb 17, 2005
Published online: Dec 1, 2005
Published in print: Dec 2005
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