TECHNICAL PAPERS
Dec 1, 2001

Assessing Multicomponent DNAPL Biostabilization Potential. II: Aroclor 1242

Publication: Journal of Environmental Engineering
Volume 127, Issue 12

Abstract

Aroclors are dense nonaqueous-phase liquids (DNAPLs) composed of polychlorinated biphenyls, which are common subsurface contaminants. Because complete remediation of Aroclor is very difficult, biostabilization may offer an alternative where risk reduction can be achieved without destruction of the DNAPL mass. The potential for aerobic in situ biostabilization of Aroclor 1242 was evaluated using laboratory protocols similar to those described in the companion paper. Total microbial concentrations increased and stabilized in both mixed and unmixed systems, while the respiring cells did not stabilize in either system. After 100 days, the DNAPL in mixed biometers was depleted in dichlorobiphenyls; the DNAPL composition in unmixed biometers did not change significantly. The total aqueous polychlorinated biphenyl concentration was lower in the unmixed than mixed biometers; both were below the predicted equilibrium concentration. After 100 days, the chronic toxicity of the aqueous phase to Cerodaphnia was greater in the biotreated systems than in the unbiotreated systems. The results indicate that aerobic microbiological activity may be insufficient to fully stabilize Aroclor in the subsurface, in contrast to the clear biostabilization potential of coal tar.

Get full access to this article

View all available purchase options and get full access to this article.

References

1.
Alder, A., Haggblom, M., Oppenheimer, S., and Young, L. ( 1993). “Reductive dechlorination of polychlorinated biphenyls in anaerobic sediments.” Environ. Sci. and Technol., 27(3), 530–538.
2.
American Public Health Association (APHA). ( 1995). Standard methods for the examination of water and wastewater, 19th Ed., A. D. Eaton, L. S. Clesceri, and A. E. Greenberg, eds., Washington, D.C.
3.
Bedard, D., Unterman, R., Bopp, L. H., Brennan, M. J., Haberl, M. L., and Johnson, C. ( 1986). “Rapid assay for screening and characterizing microorganisms for the ability to degrade polychlorinated biphenyl.” Appl. Envir. Microbiol., 51(4), 761–768.
4.
Bedard, D. L., Wagner, R. E., Brennan, M. J., Haberl, M. L., and Brown, J. F. Jr. ( 1987). “Extensive degradation of aroclors and environmentally transformed polychlorinated biphenyls by alcaligenes eutrophus H850.” Appl. Envir. Microbiol., 53(5), 1094–1102.
5.
Bhupathiraju, V. K., Hernandez, M., Krauter, P., and Alvarez-Cohen, L. ( 1999a). “A new direct microscopy based method for evaluating in-stu bioremediation.” J. Haz. Mat., B67, 299–312.
6.
Bhupathiraju, V. K., Hernandez, M., Landfear, D., and Alvarez-Cohen, L. ( 1999b). “Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria.” J. Microbiology Methods, 37, 231–243.
7.
Chu, S., He, Y., and Xu, X. ( 1997). “Determination of acute toxicity of polychlorinated biphenyls to photobacterium phosphoreum.” Bull. Envir. Contaminant Toxicology, 58, 263–267.
8.
Dillon, T. M., and Burton, W. D. S. ( 1991). “Acute Toxicity of PCB Congeners to Daphnia magna and Pimephales promelas.” Bull. Envir. Contaminant Toxicology, 46, 208–215.
9.
Erickson, M. D. ( 1997). Analytical chemistry of PCBs, Lewis, Boca Raton, Fla.
10.
Ghoshal, S., Ramaswami, A., and Luthy, R. G. ( 1996). “Biodegradation of naphthalene from coal tar and heptamethylnonane in mixed batch systems.” Envir. Sci. and Technol., 30(4), 1282–1289.
11.
Harding, G. C., and Addison, R. F. ( 1986). “Accumulation and effects of PCBs in marine invertebrates and vertebrates.” PCBs and the environment, Volume II, J. S. Waid, ed., CRC, Boca Raton, Fla.
12.
Hayes, M. A. ( 1987). “Carcinogenic and mutagenic effects of PCBs.” Environmental toxin series 1, polychlorinated biphenyls (PCBs): Mammalian and environmental toxicology, S. Safe and O. Hutzinger, eds., Springer, New York.
13.
Hutzinger, O., Safe, S., and Zitko, V. ( 1980). The chemistry of PCBs, CRC, Boca Raton, Fla.
14.
Johansen, P. ( 1996). “Biodegradation and toxicity assessment of coal-tar: Potential for biostabilization.” MS thesis, Colorado School of Mines, Golden, Colo.
15.
Luthy, R. G., Dzombak, D. A., Shannon, M. J. R., Unterman, R., and Smith, J. R. ( 1997). “Dissolution of PCB congeners from an Aroclor and an Aroclor/hydraulic oil mixture.” Water Res., 31(3), 561–573.
16.
Montgomery, J. H., and Welkom, L. W. ( 1990). Groundwater chemicals desk reference, Lewis, Chelsea, Md., 447–450.
17.
Mullin, M. D., Pochini, C. M., McCrindle, S., Romkes, M., Safe, S. H., and Safe, L. M. ( 1984). “High-resolution PCB analysis: Synthesis and chromatographic properties of all 209 PCB congeners.” Envir. Sci. and Technol., 18(6), 468–476.
18.
Nies, L., and Vogel, T. M. ( 1990). “Effects of organic substrate on dechlorination of Aroclor 1242 in anaerobic sediments.” Appl. Envir. Microbiology, 56(9), 2612–2617.
19.
Pratt, J. R., and Kepner, R. L. ( 1994). “Use of fluorochromes for direct enumeration of total bacteria in environmental samples: Past and present.” Microbiology Rev., 58, 603–615.
20.
Quensen, J. F., Mousa, M. A., Boyd, S. A., Sanderson, J. T., Froese, K. L., and Giesy, J. P. ( 1998). “Reduction of aryl hydrocarbon receptor-mediated activity of polychlorinated biphenyl mixtures due to anaerobic microbial dechlorination.” Envir. Toxicology Chem., 17, 806–813.
21.
Ramaswami, A., Johansen, P. J., Isleyen, M., Bielefeldt, A., and Illangasekare, T. (2001). “Assessing multicomponent DNAPL biostabilization. I: Coal tar.”J. Envir. Engrg., ASCE, 127(12), 1065–1072.
22.
Schultz, D. E., Petrick, G., and Duinker, J. C. ( 1989). “Complete characterization of polychlorinated biphenyl congeners in commercial Aroclor and Clophen mixtures by multidimensional gas chromatography-electron capture detection.” Envir. Sci. and Technol., 23(7), 852–859.
23.
EPA. ( 1990). “Guidance on remedial actions for superfund sites with PCB contamination.” EPA/540/G-90/007 Rep., Office of Emergency and Remedial Response, Washington, D.C.

Information & Authors

Information

Published In

Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 127Issue 12December 2001
Pages: 1073 - 1079

History

Received: Jul 18, 2000
Published online: Dec 1, 2001
Published in print: Dec 2001

Permissions

Request permissions for this article.

Authors

Affiliations

Asst. Prof., Dept. of Civ., Envir., and Arch. Engrg., Univ. of Colorado, Boulder, CO 80309-0428 (corresponding author). E-mail: bielefel @stripe.colorado.edu
Envir. Engr., Cameron-Cole, LLC, 5777 Central Ave., Ste. 100, Boulder, CO 80301.
Asst. Prof., Dept. of Civ. Engrg., Univ. of Colorado, Denver, CO 80217.
Prof., Envir. Sci. and Engrg., Colorado School of Mines, Golden, CO 80401-1887.

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share