Thermophilic Aerobic Wastewater Treatment in Continuous-Flow Bioreactors
Publication: Journal of Environmental Engineering
Volume 126, Issue 8
Abstract
Thermophilic aerobic biological wastewater treatment was investigated at 55°C in continuous-flow bioreactors using a synthetic wastewater containing gelatin and α-lactose as principal organic constituents. By operating multiple continuous-flow bioreactors at different hydraulic residence times (HRTs), the maintenance constant (kd) was calculated to be 0.046 day−1, a value approximately 10-fold lower than that previously reported for thermophilic processes but similar to that for mesophilic treatment. Investigation into the bacterial community structure by denaturing gradient gel electrophoresis of polymerase chain reaction-amplified 16S ribosomal RNA genes revealed that the dominant phylotypes in the bioreactors changed as a function of HRT. Cell reactivity, measured as the specific content of protein and RNA, declined as HRT increased. Catabolic enzyme activities specific to the principal organics in the synthetic wastewater, however, increased at higher HRTs. In conclusion, the performance of thermophilic aerobic biological wastewater treatment processes was similar to that of conventional mesophilic processes with respect to maintenance requirements (i.e., cell yields) as well as bacterial community shifts, cell reactivity, and cell activity.
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References
1.
American Public Health Association, Water Environment Federation, and American Water Works Association. ( 1995). Standard methods for the examination of water and wastewater, 19th Ed., Washington, D.C.
2.
Biebl, H., and Pfennig, N. ( 1981). “Isolation of members of the family Rhodospirillacaea.” The prokaryotes, M. P. Starr, H. Stolp, H. G. Truper, A. Balows, and H. G. Schlegel, eds., Springer, Berlin, 267–273.
3.
Cometta, S., Sonnleitner, B., and Fiechter, A. (1982). “The growth behaviour of Thermus aquaticus in continuous cultivation.” Eur. J. Appl. Microbiol. Biotechnol., Berlin, Germany, 15(2), 69–74.
4.
Couillard, D., and Zhu, S. (1993). “Thermophilic aerobic process for the treatment of slaughterhouse effluents with protein recovery.” Envir. Pollution, London, 79(2), 121–126.
5.
Eichner, C. A., Erb, R. W., Timmis, K. N., and Wagner-Döbler, I. (1999). “Thermal gradient gel electrophoresis analysis of bioprotection from pollutant shocks in the activated sludge microbial community.” Appl. Envir. Microbiology, 65(1), 102–109.
6.
Ferris, M. J., and Ward, D. M. (1997). “Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis.” Appl. Envir. Microbiology, 63(4), 1375–1381.
7.
Harder, W., and Dijkhuizen, L. (1983). “Physiological responses to nutrient limitation.” Annu. Rev. Microbiol., 37, 1–23.
8.
Hartee, E. F. (1972). “Determination of protein: A modification of the Lowry method that gives a linear photometric response.” Analytical Biochem., 48, 422–427.
9.
Herbert, D., Phipps, R. J., and Strange, R. E. (1971). “Chemical analysis of microbial cells.” Methods Microbiol., 5B, 209–234.
10.
Jackson, M. L. (1983). “Thermophilic treatment of a high-biochemical oxygen demand wastewater: Laboratory, pilot-plant and design.” Proc., 37th Purdue Industrial Waste Conf., Ann Arbor Science Publishers, Ann Arbor, Mich., 753–763.
11.
Konopka, A., Zakharova, T., Oliver, L., and Turco, R. F. (1977). “Microbial biodegradation of organic wastes containing surfactants in a continuous-flow reactor.” J. Industrial Microbiol. Biotechnol., Hampshire, U.K., 18(4), 235–240.
12.
Konopka, A., Zakharova, T., Oliver, L., Paseuth, E., and Turco, R. F. (1998). “Physiological state of a microbial community in a biomass recycle reactor.” J. Industrial Microbiol. Biotechnol., Hampshire, U.K., 20(3–4), 232–237.
13.
Konopka, A., Zakharova, T., and LaPara, T. M. (1999). “Bacterial function and community structure in reactors treating biopolymers and surfactants at mesophilic and thermophilic temperatures.” J. Industrial Microbiol. Biotechnol., Hampshire, U.K., 23(2), 127–132.
14.
Lane, D. J. ( 1991). “16S/23S rRNA sequencing.” Nucleic acid techniques in bacterial systematics, E. Stackebrandt and M. Goodfellow, eds., Wiley, New York, 115–175.
15.
LaPara, T. M., and Alleman, J. E. (1999). “Thermophilic aerobic biological wastewater treatment.” Water Res., 33(4), 895–908.
16.
Lawrence, A. W., and McCarty, P. L. (1970). “Unified basis for biological treatment design and operation.”J. Sanit. Engrg. Div., ASCE, 96(3), 757–778.
17.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). “Protein measurement with the Folin phenol reagent.” J. Biol. Chem., 193, 265–275.
18.
Marr, A. G., Nilson, E. H., and Clark, D. J. (1963). “The maintenance requirement of Escherichia coli.” Ann. N.Y. Acad. Sci., 102, 536–548.
19.
Matin, A. ( 1979). “Microbial regulatory mechanisms at low nutrient concentrations as studied in chemostat.” Strategies of microbial life in extreme environments, Shilo, M., ed., Verlag Chemie, Weinheim, Germany, 323–339.
20.
Muyzer, G., Dewaal, E. C., and Uitterlinden, A. G. (1993). “Profiling of complex microbial populations by denaturing gradient gel electrophoresis of polymerase chain reaction-amplified genes coding for 16S rRNA.” Appl. Envir. Microbiology, 59(3), 695–700.
21.
Neidhardt, F. C., Ingraham, J. L., and Schaechter, M. (1990). Physiology of the bacterial cell, Sinaeuer, Sunderland, Mass.
22.
Pennock, J., and Tempest, D. W. (1988). “Metabolic and energetic aspects of the growth of Bacillus stearothermophilus in glucose-limited and glucose-sufficient chemostat culture.” Archives of Microbiology, 133, 300–302.
23.
Powell, E. O. ( 1969). “Transient changes in the growth rate of microorganisms.” Continuous cultivation of microorganisms, I. Malek, ed., Academia, Prague, 275–284.
24.
Rozich, A. F., and Colvin, R. J. (1997). “Design and operational considerations for thermophilic aerobic reactors treating high strength wastes and sludges.” Proc., 52nd Purdue Industrial Waste Conf., Ann Arbor Press, Ann Arbor, Mich., 1–6.
25.
Sonnleitner, B., Cometta, S., and Fiechter, A. (1982). “Growth kinetics of Thermus thermophilus.” Eur. J. Appl. Microbiol. Biotechnol., 15(2) 75–82.
26.
Sorensen, T. (1948). “A method of establishing groups of equal amplitude in plant sociology based on similarity of species content.” Biol. Skr., 4, 1–34.
27.
Sürücü, G. A., Chian, E. S. K., and Engelbrecht, R. S. (1976). “Aerobic thermophilic treatment of high strength wastewaters.” J. Water Pollution Control Fedn., 48(4), 669–679.
28.
Stover, E. L. (1999). “Aerobic autoheated thermophilic treatment process for high strength industrial waste residuals.” Proc., Industrial Wastes Tech. Conf., W. J. Librizzi, and J. M. Shoemake, eds., Water Environment Federation, Alexandria, Va.
29.
Tchobanoglous, G., and Burton, F. L., eds. (1991). Wastewater engineering: Treatment, disposal, and reuse, 3rd Ed., McGraw-Hill, New York.
30.
Tempest, D. W., Neijssel, O. M., and Zevenboom, W. (1983). “Properties and performances in laboratory cultures; their relevance to growth in natural ecosystems.” Symp. Soc. Gen. Microbiol., 34, 119–152.
31.
U.S. EPA. (1990). “Environmental regulation and technology: Autothermal thermophilic aerobic digestion of municipal wastewater sludge.” EPA/625/10-90/007, Cincnnati, Ohio.
32.
Watanabe, K., Teramoto, M., and Harayama, S. (1999). “An outbreak of nonflocculating catabolic populations caused the breakdown of a phenol-digesting activated-sludge process.” Appl. Envir. Microbiology, 65(7), 2813–2819.
Information & Authors
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Published In
Journal of Environmental Engineering
Volume 126 • Issue 8 • August 2000
Pages: 739 - 744
History
Received: Sep 20, 1999
Published online: Aug 1, 2000
Published in print: Aug 2000
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