TECHNICAL PAPERS
Jul 1, 2000

Bacterial Transport in NAPL-Contaminated Porous Media

Publication: Journal of Environmental Engineering
Volume 126, Issue 7

Abstract

To understand the effect of a non-aqueous-phase liquid (NAPL) on bacterial transport, column experiments in the presence and absence of a NAPL were conducted using two bacterial strains and two different porous media. The presence of a NAPL (tetrachloroethene) decreased the retention of Pseudomonas fluorescens P17 in quartz and soil columns (factor of 2 for quartz and 1.6 for the soil). In contrast, there was little change in the overall transport of P. putida KT2442 in soil columns in the presence of a NAPL. To understand how a NAPL phase might affect bacterial transport, several different mechanisms of particle removal by a NAPL were hypothesized, and a filtration model was modified to test each hypothesis. Only one of the five models was consistent with the increased transport of P17 in the presence of the NAPL suggesting that the NAPL produced large, immobile zones of water. The presence of immobile water zones would decrease overall porosity, reduce the number of packing grains available for particle filtration, and increase water velocity, resulting in increased bacterial transport in the presence of a NAPL.

Get full access to this article

View all available purchase options and get full access to this article.

References

1.
Albinger, O., Biesemeyer, B. K., Arnold, R. G., and Logan, B. E. (1994). “Effect of bacterial heterogeneity on adhesion to uniform collectors by monoclonal populations.” FEMS Microbiol. Lett., 124, 321–326.
2.
Camesano, T. A., and Logan, B. E. (1998). “Influence of fluid velocity and cell concentration on the transport of motile and non-motile bacteria in porous media.” Envir. Sci. and Technol., 32, 1699–1708.
3.
Fletcher, M., and Floodgate, G. D. (1973). “An electron-microscopic demonstration of an acid polysaccharide involved in the adhesion of a marine bacterium to solid surfaces.” J. Gen. Microbiol., 74, 325– 334.
4.
Gannon, J., Tan, Y., Baveye, P., and Alexander, M. (1991). “Effect of sodium chloride on transport of bacteria in a saturated aquifer material.” Appl. Envir. Microbiology, 57, 2497–2501.
5.
Gerba, C. P., and Bitton, G. ( 1984). “Microbial pollutants, their survival and transport pattern to groundwater.” Groundwater pollution microbiology, C. P. Gerba, ed., Wiley, New York, 53–67.
6.
Gross, M. J., and Logan, B. E. (1995). “Influence of different chemical treatments on transport of Alcaligenes paradoxus in porous media.” Appl. Envir. Microbiology, 61, 1750–1756.
7.
Hobbie, J. E., Daley, R. J., and Jasper, S. (1977). “Use of nucleopore filters for counting bacteria by fluorescence microscopy.” Appl. Envir. Microbiology, 33, 1225–1228.
8.
Hunt, J. R., Sitar, N., and Udell, K. S. (1988). “Nonaqueous phase liquid transport and cleanup. 1. Analysis of mechanisms.” Water Resour. Res., 24, 1247–1258.
9.
Jewett, D. G., Hilbert, T. A., Logan, B. E., Arnold, R. G., and Bales, R. C. (1995). “Bacterial transport in columns and filters: Influence of ionic strength and pH on collision efficiency.” Water Res., 29, 1673–1680.
10.
Jin, M., et al. (1995). “Partitioning tracer test for the detection, estimation, and remediation performance assessment of subsurface nonaqueous phase liquids.” Water Resour. Res., 31, 1201–1211.
11.
Johnson, W. P., Martin, M. J., Gross, M. J., and Logan, B. E. (1996). “Facilitation of bacterial transport through porous media by changes in solution and surface properties.” Colloids and Surfaces A: Physiochem. Engrg. Aspects, 107, 263–271.
12.
Jucker, B. A., Zehnder, A. J. B., and Harms, H. (1998). “Quantification of polymer interactions in bacterial adhesion.” Envir. Sci. and Technol., 32, 2909–2915.
13.
Kinoshita, T., Bales, R. C., Yahya, M. T., and Gerba, C. P. (1993). “Bacterial transport in a porous medium: Retention of Bacillus and Pseudomonas on silica surfaces.” Water Res., 27, 1295–1301.
14.
Li, Q., and Logan, B. E. (1999). “Enhancing bacterial transport for bioaugmentation of aquifers using low ionic strength solutions and surfactants.” Water Res., 33, 1090–1100.
15.
Logan, B. E. (1999). Environmental transport processes, Wiley, New York.
16.
Logan, B. E., Jewett, D. G., Arnold, R. G., Bouwer, E. J., and O'Melia, C. R. (1995). “Clarification of clean-bed filtration models.”J. Envir. Engrg., ASCE, 121(12), 869–873.
17.
McCaulou, D. R., Bales, R. C., and Arnold, R. G. (1995). “Effect of temperature-controlled motility on transport of bacteria and microspheres through saturated sediment.” Water Resour. Res., 31, 271– 280.
18.
Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982). Molecular cloning: A laboratory manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19.
Marlow, H. J., Duston, K. L., Wiesner, M. R., Thompson, M. T., Wilson, J. T., and Ward, C. H. (1991). “Microbial transport through porous media: The effects of hydraulic conductivity and injection velocity.” J. Haz. Mat., 28, 65–74.
20.
Martin, M. J., Logan, B. E., Johnson, W. P., Jewett, D. G., and Arnold, R. G. (1996). “Scaling bacterial filtration rates in different sized porous media.”J. Envir. Engrg., ASCE, 122(5), 407–415.
21.
Nüßlein, K., Maris, D., Timmis, K., and Dwyer, D. F. (1992). “Expression and transfer of engineered catabolic pathways harbored by Pseudomonas spp. introduced into activated sludge microcosms.” Appl. Envir. Microbiology, 58, 3380–3386.
22.
Pennell, K. D., Jin, M., Abriola, L. M., and Pope, G. A. (1994). “Surfactant enhanced remediation of soil columns contaminated by residual tetrachloroethylene.” J. Contam. Hydrol., 16, 35–53.
23.
Rajagopalan, R., and Tien, C. (1976). “Trajectory analysis of deep-bed filtration with the sphere-in-cell porous media model.” AIChE J., 22, 523–533.
24.
Rogers, B. ( 1997). “Bacterial transport through a NAPL contaminated porous media.” MS thesis, Dept. of Chemical and Envir. Engrg., University of Arizona, Tucson, Ariz.
25.
Rosenberg, M. (1984). “Bacterial adherence to hydrocarbons: A useful technique for studying cell surface hydrophobicity.” FEMS Microbiol. Lett., 22, 289–295.
26.
Runkel, R. L. (1996). “Solution of the advection-dispersion equation: Continuous load of finite duration.”J. Envir. Engrg., ASCE, 122(9), 830–832.
27.
Scholl, M. A., and Harvey, R. W. (1992). “Laboratory investigations on the roles of sediment surface and groundwater chemistry on the attachment of bacteria to representative aquifer material.” J. Contam. Hydrol., 6, 321–336.
28.
Sharma, M. M., Chang, Y. I., and Yen, T. F. (1985). “Reversible and irreversible surface charge modification of bacteria for facilitating transport through porous media.” Colloids Surf., 16, 193–206.
29.
Shonnard, D. R., Taylor, R. T., Hanna, M. L., Boro, C. O., and Duba, A. G. (1994). “Injection-attachment of Methylosinus trichosporium OB3b in a two-dimensional miniature sand-filled aquifer simulator.” Water Resour. Res., 30, 25–35.
30.
Stenström, T. A. (1989). “Bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles.” Appl. Envir. Microbiology, 55, 142–147.
31.
van Loosdrecht, M. C. M., Lyklema, J., Norde, W., Schraa, G., and Zehnder, A. J. B. (1987a). “The role of bacterial cell wall hydrophobicity in adhesion.” Appl. Envir. Microbiology, 53, 1893–1897.
32.
van Loosdrecht, M. C. M., Lyklema, J., Norde, W., Schraa, G., and Zehnder, A. J. B. (1987b). “Electrophoretic mobility and hydrophobicity as a measure to predict the initial steps of bacterial adhesion.” Appl. Envir. Microbiology, 53, 1898–1901.
33.
Wagner-Dobler, I., Pipke, R., Timmis, K. N., and Dwyer, D. F. (1992). “Evaluation of aquatic sediment microcosms and their use in assessing possible effects on introduced microorganisms on ecosystem parameters.” Appl. Envir. Microbiology, 58, 1249–1258.
34.
Yao, K. M., Habibibian, T., and O'Melia, C. R. (1971). “Water and waste water filtration: Concepts and applications.” Envir. Sci. and Technol., 5, 1105–1112.
35.
Yates, M. V. (1988). “Modeling microbial fate in the subsurface environment.” Crit. Rev. Envir. Cont., 17, 307–344.

Information & Authors

Information

Published In

Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 126Issue 7July 2000
Pages: 657 - 666

History

Received: Jul 23, 1999
Published online: Jul 1, 2000
Published in print: Jul 2000

Permissions

Request permissions for this article.

Authors

Affiliations

Member, ASCE
Res. Assoc., Dept. of Chem. and Envir. Engrg., Univ. of Arizona, Tucson, AZ 85721; currently, URS Greiner Woodward Clyde, 7878 North 16th St., Ste. 200, Phoenix, AZ 85020.
Prof., Dept. of Civ. and Envir. Engrg., Pennsylvania State Univ., University Park, PA 16802; Corresponding author E-mail: [email protected]

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share