Soluble Microbial Products in ABR Treating Low-Strength Wastewater
This article has been corrected.
VIEW CORRECTIONPublication: Journal of Environmental Engineering
Volume 126, Issue 3
Abstract
The chemical composition, molecular weight (MW) distribution, and biodegradability (both aerobic and anaerobic) of soluble microbial products (SMPs) in an anaerobic baffled reactor (ABR) treating low-strength wastewater were investigated. The effect of various process parameters on the production of SMPs was also examined. Results indicated that high MW (>300 kDa) compounds were produced in the middle compartments of the reactor and formed 22% of the effluent chemical oxygen demand (COD). This fraction was found to be 86% degradable under aerobic conditions but only 4% under anaerobic conditions. Low MW (<1 kDa) material represented the highest portion (36%) of the effluent COD and was mainly found in the first compartment of the ABR and in the effluent. This fraction was more easily degraded under anaerobic conditions (33%) than aerobic conditions (17%). Analysis of a hydrolyzed sample of the high MW fraction revealed the presence of several sugars and volatile fatty acids. Therefore, it was concluded that the high MW material contains heteropolysaccharides. Nuclear magnetic resonance analysis of the low MW fraction revealed the possible presence of alcohol, carboxylate, and aromatic chemical groups. SMP production increased with increasing hydraulic retention time (HRT), probably due to enhanced biomass decay at high HRTs, and also increased with decreasing temperature, probably due to increased stress on the biomass and a reduced metabolism of the SMP at low temperatures. Finally, SMP production in an ABR containing higher levels of initial biomass concentration was greater than for an ABR operating at the same conditions but with lower levels of initial biomass.
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References
1.
Akitt, J. W. (1992). NMR and chemistry: An introduction to modern NMR spectroscopy, 3rd ed. Chapham and Hall, London.
2.
Amy, G. L., Collins, M. R., Kuo, C. J., and King, P. H. (1987). “Comparing gel permeation and ultrafiltration for the molecular weight characterization of aquatic organic matter.” J. AWWA, 79(1), 43–49.
3.
Barber, W. P., and Stuckey, D. C. (1999). “The use of the anaerobic baffled reactor (ABR) for wastewater treatment: A review.” Water Res., 33(7), 1557–1578.
4.
Barker, D. J., Mannucchi, G., Salvi, S. M. L., and Stuckey, D. C. (1999). “Characterisation of soluble residual chemical oxygen demand (COD) in anaerobic wastewater treatment effluents.” Water Res., 33(11), 2499–2510.
5.
Barker, D. J., and Stuckey, D. C. (1999). “A review of soluble microbial products (SMP) in wastewater treatment systems.” Water Res., 33(14), 3063–3082.
6.
Bunch, R. L., Barth, E. F., and Ettinger, M. B. (1961). “Organic materials in secondary effluents.” J. Water Pollution Control Federation, 33(2), 122–126.
7.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956). “Colorimetric method for determination of sugars and related substances.” Analytical Chemistry, 28, 350–356.
8.
Hejzlar, J., and Chudoba, J. (1986a). “Microbial polymers in the aquatic environment. I: Production by activated sludge microorganisms under different conditions.” Water Res., 20(10), 1209–1216.
9.
Hejzlar, J., and Chudoba, J. (1986b). “Microbial polymers in the aquatic environment. II: Isolation from biologically non-purified and purified municipal waste water analysis.” Water Res., 20(10), 1217–1221.
10.
Kato, M. T., Field, J. A., Kleerebezem, R., and Lettinga, G. (1994). “Treatment of low strength soluble wastewaters in UASB reactors.” J. Fermentation Bioengrg., 77(6), 679–686.
11.
Kato, M. T., Field, J. A., and Lettinga, G. (1997). “The anaerobic treatment of low strength wastewaters in UASB and EGSB reactors.” Water Sci. Technol., 36(6–7), 375–382.
12.
Kenne, L., and Lindberg, B. ( 1983). “Bacterial polysaccharides,” The Polysaccharides, Vol. 2, G. O. Aspinall, ed., Academic Press, New York, 287–363.
13.
Kida, K., Tanemura, K., Ohno, A., and Sonoda, Y. (1991). “Comparison of performance among four different processes for the anaerobic treatment of wastewater with a low concentration of organic matter.” Envir. Technol., 12, 497–502.
14.
Kobayashi, H. A., Stenstrom, M. K., and Mah, R. A. (1983). “Treatment of low strength domestic wastewater using the anaerobic filter.” Water Res., 17(8), 903–909.
15.
Kuo, W. C., and Parkin, G. F. (1996). “Characterization of soluble microbial products from anaerobic treatment by molecular weight distribution and nickel-chelating properties.” Water Res., 30(4), 915–922.
16.
Kuo, W. C., Sneve, M. A., and Parkin, G. F. (1996). “Formation of soluble microbial products during anaerobic treatment.” Water Envir. Res., 68, 279–285.
17.
Langenhoff, A. A. M., Intrachandra, N., and Stuckey, D. C. (2000). “The anaerobic treatment of dilute soluble and colloidal wastewater using an anaerobic baffled reactor: Effects of short hydraulic retention.” Water Res., 30(4), 1304–1317.
18.
Lettinga, G., Hobma, S. W., Hulshoff Pol, L. W., de Zeeuw, W., de Jong, P., and Roersma, R. (1983). “Design, operation and economy of anaerobic treatment.” Water Sci. Technol., 15(8–9), 177–195.
19.
Nachaiyasit, S., and Stuckey, D. C. (1997). “Effect of low temperatures on the performance of an anaerobic baffled reactor (ABR).” J. Chem. Technol. Biotech., 69, 276–284.
20.
Noguera, D. R., Araki, N., and Rittmann, B. E. (1994). “Soluble microbial products (SMP) in anaerobic chemostats.” Biotech. Bioengrg., 44, 1040–1047.
21.
Ohnishi, S. T., and Barr, J. K. (1978). “A simplified method of quantitating proteins using the biuret and phenol reagents.” Analytical Biochemistry, 86, 193–200.
22.
Owen, W. F., Stuckey, D. C., Healy, J. B. Jr., Young, L. Y., and McCarty, P. L. (1979). “Bioassays for monitoring biochemical methane potential and anaerobic toxicity.” Water Res., 13, 485–492.
23.
Painter, H. A. (1973). “Organic compounds in solution in sewage effluents.” Chem. and Industry, London, September, 818–822.
24.
Schiener, P., Nachaiyasit, S., and Stuckey, D. C. (1998). “Production of soluble microbial products (SMP) in an anaerobic baffled reactor: Composition, biodegradability and the effect of process parameters.” Envir. Technol., 19, 391–400.
25.
Sollfrank, U., Kappeler, J., and Gujer, W. (1992). “Temperature effects on wastewater characterisation and the release of soluble inert organic material.” Water Sci. Technol., 25(6), 33–41.
26.
Speece, R. E. (1996). Anaerobic biotechnology for industrial wastewaters. Archae Press, Nashville, Tenn.
27.
Standard methods for the examination of water and wastewater, 18th ed. (1992). American Public Health Association, Washington, D.C.
28.
Urbain, V., Mobarry, B., de Silva, V., Stahl, D. A., Rittmann, B. E., and Manem, J. (1998). “Integration of performance, molecular biology and modeling to describe the activated sludge process,” Water Sci. Technol., 37, 223–229.
29.
Voolapalli, R. K. ( 1999). “Stability enhancement of anaerobic digestion through intermediate product regulation,” PhD thesis, Imperial College of Science, Technology and Medicine, London.
Information & Authors
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Published In
Journal of Environmental Engineering
Volume 126 • Issue 3 • March 2000
Pages: 239 - 249
History
Received: Dec 8, 1998
Published online: Mar 1, 2000
Published in print: Mar 2000
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