Chromium(VI) Reduction by Pseudomonas fluorescens LB300 in Fixed-Film Bioreactor
Publication: Journal of Environmental Engineering
Volume 123, Issue 8
Abstract
The potential for Cr(VI) reduction in a bench-scale, fixed-film bioreactor system was investigated using a Cr(VI) reducing bacterial species, Pseudomonas fluorescens LB300. The bench-scale reactor was packed by glass beads and was operated to steady-state conditions with near complete removal of Cr(VI) under a range of influent Cr(VI) concentrations (30–100 mg/L) and hydraulic retention times (3.6–14.2 min). Cr(VI) overloadings were observed when the bioreactor system was operated at 200 mg Cr(VI)/L under a 14.2 min hydraulic retention time and at 50 mg Cr(VI)/L under a 3.6 min hydraulic retention time. The system recovered in both cases after reducing Cr(VI) loadings by lowering influent Cr(VI) concentration to 10 mg/L. Cr(VI) reduction was carried out by biological mechanisms through both attached and suspended biomass. Control studies using Cr(VI) showed that abiotic Cr(VI) reduction in the bioreactor was insignificant. Nearly all the influent Cr(VI) reduced in the reactor was recovered in the form of Cr(III) in the effluent.
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References
1.
Bopp, L. H., Chakrabbarty, A. M., and Ehrlich, H. L.(1983). “Chromate resistance in Pseudomonas fluorescens.”J. Bacteriology, 155(3), 1105–1109.
2.
Bopp, L. H., and Ehrlich, H. L.(1988). “Chromate resistance and reduction in Pseudomonas fluorescens strain LB300.”Archives of Microbiol., 150, 426–431.
3.
Caldwell, D. E., and Lawrence, J. R.(1986). “Growth kinetics of Pseudomonas fluorescens microcolonies within the hydrodynamic boundary layers of surface microenvironments.”Microbial Ecology, 12(3), 299–312.
4.
Chang, S. Y., Huang, J. C., and Liu, Y. C.(1986). “Effects of Cd(II) and Cu(II) on a biofilm system.”J. Envir. Engrg., ASCE, 112(1), 94–104.
5.
Chirwa, E. M. N., and Wang, Y. T.(1997). “Hexavalent chromium reduction by Bacillus sp. in a packed-bed bioreactor.”Envir. Sci. and Technol., 31(5), 1446–1451.
6.
Chuan, M. C., and Liu, J. C.(1996). “Release behavior of chromium from tannery sludge.”Water Res., 30(4), 932–938.
7.
“Determination of chromium by ion chromatography.” (1991). TN 24, LPN 034350-015M 7/91, Dionex Corp., Sunnydale, Calif.
8.
Fujii, E., Toda, K., and Ohtake, H.(1990). “Bacterial removal of toxic hexavalent chromium using a fed-batch culture of Enterobacter cloacae strain HO1.”J. Fermentation and Bioengrg., 69(6), 365–367.
9.
Hodge, J. E., and Hofreiter, B. T. (1962). “Determination of reducing sugars and carbohydrates.”Methods in carbohydrate chemistry, R. L. Whistler, ed., Vol. I, Academic Press, Inc., New York, N.Y., 392–393.
10.
Horitsu, H., Futo, S., Miyazawa, Y., Ogai, S., and Kawai, K.(1987). “Enzymatic reduction of hexavalent chromium by hexavalent chromium tolerant Pseudomonas ambigua G-1.”Agric. Biol. and Chem., 51(9), 2417–2420.
11.
Ishibashi, Y., Cervantes, C., and Silver, S.(1990). “Chromium reduction in Pseudomonas putida.”Appl. and Envir. Microbiol., 56(7), 2268–2270.
12.
Keith, L. H., and Tilliard, W. A.(1979). “Priority pollutants I—a perspective review.”Envir. Sci. and Technol., 13(4), 416–423.
13.
Levenspiel, O. (1972). Chemical Reaction Engineering, 2nd Ed., John Wiley & Sons, Inc., New York, N.Y., 253–317.
14.
Mazierski, J.(1994). “Effect of chromium(VI) on the growth of denitrifying bacteria.”Water Res., 28(9), 1981–1985.
15.
Mearns, A. J., Oshida, P. S., Sherwood, M. J., Young, D. R., and Reish, D. J.(1976). “Chromium effects on coastal organisms.”J. Water Pollution Control Fed., 48(8), 1929–1939.
16.
Nishioka, H.(1975). “Mutagenic activities of metal compounds in bacteria.”Mutation Res., 31, 185–190.
17.
Pettrilli, F. L., and DeFlora, S.(1977). “Toxicity and mutagenicity of hexavalent chromium on Salmonella typhimuruim.”Appl. and Envir. Microbiol., 33(4), 805–809.
18.
Shen, H., and Wang, Y. T.(1993). “Characterization of enzymatic reduction of hexavalent chromium by Escherichia coli ATCC 33456.”Appl. and Envir. Microbiol., 59(11), 3771–3777.
19.
Shen, H., and Wang, Y. T.(1994). “Modeling hexavalent chromium reduction in Escherichia coli ATCC 33456.”Biotechnol. and Bioengrg., 43(4), 293–300.
20.
Shen, H., and Wang, Y. T.(1995). “Hexavalent chromium removal in a two-stage bioreactor system.”J. Envir. Engrg., ASCE, 121(11), 798–804.
21.
“Standard methods for the examination of water and wastewater.” (1992). 18th Ed., 3-5, 3-59, 5-2, and 9-35, American Public Health Association (APHA) Publication Office, Washington, D.C.
22.
Suzuki, Y., and Fukuda, K.(1990). “Reduction of hexavalent chromium by ascorbic acid and glutathione with special reference to the rat lung.”Archives of Toxicology, 64(3), 169–176.
23.
Wang, Y. T., and Shen, H.(1995). “Bacterial reduction of hexavalent chromium: A review.”J. Industrial Microbiol., 14(2), 154–163.
24.
Wang, Y. T., and Xiao, C.(1995). “Factors affecting hexavalent chromium reduction in pure cultures of bacteria.”Water Res., 29(11), 2467–2474.
25.
Yassi, A., and Nieboer, E. (1988). Chromium in natural and human environments, J. O. Nriagu and E. Nieboer, eds., Wiley-Interscience, New York, N.Y., 443–495.
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Copyright © 1997 American Society of Civil Engineers.
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Published online: Aug 1, 1997
Published in print: Aug 1997
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