Soil Gas Oxygen Tension and Pentachlorophenol Biodegradation
Publication: Journal of Environmental Engineering
Volume 123, Issue 4
Abstract
Laboratory tests were conducted to determine the effect of soil gas oxygen concentration on the degradation and mineralization of spiked 14C-pentachlorophenol and nonlabeled pentachlorophenol (PCP) present in soil taken from a prepared-bed land treatment unit at the Champion International Superfund Site in Libby, Mont. This soil was contaminated with wood preserving wastes including creosote and PCP. Degradation rates of 14C-PCP and nonlabeled PCP were found to be enhanced under soil gas oxygen concentrations between 2 and 21% in the contaminated soil. Between 48 and 64% of 14C-PCP spiked onto the soil was mineralized after 70 days at soil gas oxygen levels between 2 and 21%. No statistically significant mineralization of PCP was found to occur at 0% oxygen concentrations. Mineralization of 14C-PCP in contaminated soil poisoned with mercuric chloride was determined to be less than 0.2%. Degradation of indigenous nonradiolabeled PCP in the nonpoisoned soil was statistically significantly greater than in poisoned soil. These results indicated that degradation of PCP was biological and would occur under low oxygen concentrations. Soil gas oxygen concentrations necessary for PCP biodegradation (2–5%) could be maintained, for example, using bioventing technology in order to achieve continued treatment of buried lifts of soil while new lifts are added, thus decreasing the total time for soil remediation of the prepared bed.
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References
1.
Bollag, J.-M., and Bollag, W. B.(1990). “A model for enzymatic binding of pollutants in the soil.”Int. J. Envir. Anal. Chem., 39(1), 147–157.
2.
Crawford, R. L., and Mohn, W. W.(1985). “Microbial removal of pentachlorophenol from soil using a Flavobacterium.”Enzyme Microb. Technol., 7(12), 617–620.
3.
Crosby, D. G.(1981). “Environmental chemistry of pentachlorophenol.”Pure Appl. Chem., 53(5), 1051–1080.
4.
Edgehill, R. U., and Finn, R. K.(1983). “Microbial treatment of soil to remove pentachlorophenol.”Appl. Envir. Microbiology, 45(3), 1122–1125.
5.
Hendriksen, H. V., Larsen, S., and Ahring, B. K.(1992). “Influence of a supplemental carbon source on anaerobic dechlorination of pentachlorophenol in granular sludge.”Appl. Envir. Microbiology, 58(1), 365–370.
6.
Huling, S. G., Pope, D. F., Matthews, J. E., Sims, J. L., Sims, R. C., and Sorensen, D. L. (1995). “Land treatment and the toxicity response of soil contaminated with wood preserving waste.”Remediation (Spring), 41–55.
7.
Kaufmen, D. D. (1978). Degradation of pentachlorophenol in soil, and by soil microorganisms. Plenum Publishing Corp, New York, N.Y.
8.
Kinzell, L. H., McKenzie, R. M., Olson, B. A., Kirsch, D. G., and Shull, L. R.(1979). “Priority pollutants. I: a perspective view.”Envir. Sci. Technol., 13(4), 416–423.
9.
Kuwatsuka, S., and Igarashi, M.(1975). “Degradation of pentachlorophenol in soils. II: The relationship between the degradation of PCP and the properties of soils, and the identification of the degradation products of PCP.”Soil Sci. Plant Nutr., 21(4), 405–414.
10.
Lamar, R. T., and Dietrich, D. M.(1990). “In situ depletion of pentachlorophenol from contaminated soil by Phenerochaete spp.”Appl. Envir. Microbiology, 56(10), 3093–3100.
11.
Lee, M. D. (1986). “Biodegradation of organic contaminants in the subsurface of hazardous waste sites,” PhD thesis, Rice Univ., Houston, Tex.
12.
Lovley, D. R.(1991). “Dissimilatory Fe III and Mn IV reduction.”Microbiology Rev., 55(2), 259–287.
13.
Madsen, T., and Licht, D.(1992). “Isolation and characterization of an anaerobic chlorophenol-transforming bacterium.”Appl. Envir. Microbiology, 58(9), 2874–2878.
14.
McConnachie, P. R., and Zahalsky, A. C.(1991). “Immunological consequences of exposure to pentachlorophenol.”Arc. of Envir. Health, 46(4), 249–253.
15.
McFarland, M. J., and Sims, R. C.(1991). “Thermodynamic framework for evaluating PAH degradation in the subsurface.”Ground Water, 29(6), 884–896.
16.
Mikesell, M. D., and Boyd, S. A.(1986). “Complete reductive dechlorination and mineralization of pentachlorophenol by anaerobic microorganisms.”Appl. Envir. Microbiology, 52(4), 861–865.
17.
Penman, H. L.(1940). “Gas and vapor movements in the soil.”J. Agric. Sci., 30, 570–581.
18.
Pope, D. F., and Matthews, J. E. (1993). “Bioremediation using the land treatment concept.”EPA 600/R-93/164, U.S. Envir. Protection Agency, Washington, D.C.
19.
Ramaprasad, A. (1994). “Limitations on biotransformation of pentachlorophenol in soil,” MSc thesis, Utah State Univ., Logan, Utah.
20.
Rao, K. R. (1978). Pentachlorophenol: chemistry, pharmacology, and environmental toxicology. Plenum Publishing Corp, New York, N.Y.
21.
Ruckdeschel, G., Renner, G., and Schwarz, K.(1987). “Effects of pentachlorophenol and some of its known an possible metabolites on different species of bacteria.”Appl. Envir. Microbiology, 53(11), 2689–2692.
22.
Sextone, A. J., Reusbech, N. P., Parkin, T., and Tiedje, J. M.(1985). “Direct measurement of oxygen profiles and denitrification rates in soil aggregates.”Soil Sci. Soc. Am. J., 49(3), 645–651.
23.
Sims, J. L., Sims, R. C., and Matthews, J. E. (1989). “Bioremediation of contaminated surface soils.”EPA 600/9-89/073, U.S. Envir. Protection Agency, Washington, D.C.
24.
Sims R. C. et al. (1986). Contaminated surface soils in-place treatment techniques. Noyes Publications, Park Ridge, N.J.
25.
Sims, R. C., Sims, J. L., Sorensen, D. L., and McLean, J. E. (1995). “Champion International Superfund site, Libby, Montana: bioremediation field performance evaluation of the prepared bed land treatment system.”EPA-600/R-95/156, U.S. Envir. Protection Agency, Robert S. Kerr Envir. Res. Lab., Ada, Okla.
26.
Stetzenbach, L. D. A. (1986). “The degradation and utilization of polycyclic aromatic hydrocarbons by indigenous soil bacteria,” PhD thesis, Univ. of Arizona, Tucson, Ariz.
27.
Stolzey, L. H., and Letey, J.(1964). “Characterizing soil oxygen conditions with platinum electrode.”Adv. in Agronomy, 16, 249–279.
28.
U.S. Environmental Protection Agency (EPA). (1978). “Report of the ad hoc study group on pentachlorophenol contaminants.”EPA/SAB/78/001, Washington, D.C.
29.
U.S. Environmental Protection Agency (EPA). (1986). “Ambient aquatic life water quality criteria for pentachlorophenol.”EPA/440/586/009, Off. of Res. and Devel., Washington, D.C.
30.
U.S. Environmental Protection Agency (EPA). (1986). “Test methods for evaluating solid waste, physical/chemical methods.”SW-846, Washington, D.C.
31.
U.S. Environmental Protection Agency (EPA). (1990). “Subsurface contamination reference guide.”EPA/540/2-90/011, Ofc. of Emergency and Remedial Response, Washington, D.C.
32.
Valo R. J., Haggblom, and M. M., and Salkinoja-Salonen(1990). “Bioremediation of chlorophenol containing simulated groundwater by immobilized bacteria.”Water Res., 24(2), 253–258.
33.
Watanabe, I.(1977). “Pentachlorophenol-decomposing and PCP-tolerant bacteria in field soil treated with PCP.”Soil Biol. and Biochemistry, 9(1), 99–103.
34.
Watanabe, I.(1978). “Pentachlorophenol-decomposing activity of field soils treated annually with PCP.”Soil Biol. and Biochemistry, 10(1), 71–75.
35.
Wolf, D. C., Dao, T. H., Scott, H. D., and Lavy, T. L.(1989). “Influence of sterilization methods on selected microbiological, physical, and chemical properties.”J. Envir. Quality, 18(1), 39–44.
Information & Authors
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Published In
Journal of Environmental Engineering
Volume 123 • Issue 4 • April 1997
Pages: 364 - 370
Copyright
Copyright © 1997 American Society of Civil Engineers.
History
Published online: Apr 1, 1997
Published in print: Apr 1997
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