Model for Treatment of Trichloroethylene by Methanotrophic Biofilms
Publication: Journal of Environmental Engineering
Volume 120, Issue 2
Abstract
A biofilm model for the cometabolic degradation of trichloroethylene (TCE) by methane oxidizing (methanotrophic) bacteria is derived. Methane utilization and TCE transformation were modeled using diffusive mass transport, Monod kinetics, competitive inhibition, TCE transformation product toxicity, and growth, decay and inactivation of the methanotrophic bacteria. Reported low rates of TCE degradation by biofilms were found to be compatible with the high rates found in dispersed growth studies. The slower rates result from phenomena inherent in biofilms, and not necessarily from a difference in performance characteristics of the organisms. The possibility that biofilms may not be copper‐limited is also considered. Other model predictions include an optimum methane concentration that maximizes TCE flux. Also, survival of a biofilm should only occur when the methane concentration is above a certain minimum value , which is linearly related to TCE concentration. The model is general and can be applied to other primary substrates and chlorinated aliphatic hydrocarbons (CAHs).
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References
1.
Alvarez‐Cohen, L. M. (1991). “Cometabolic biotransformation of trichloroethylene and chloroform by methanotrophs—experimental studies and modeling of toxicity and sorption effects.” Ph.D. dissertation, Stanford University, Stanford, Calif.
2.
Alvarez‐Cohen, L. M., and McCarty, P. L. (1991a). “A cometabolic biotransformation model for halogenated aliphatic compounds exhibiting product toxicity.” Envir. Sci. Tech., 25(8), 1381–1387.
3.
Alvarez‐Cohen, L. M., and McCarty, P. L. (1991b). “Effects of toxicity, aeration and reductant supply on trichloroethylene transformation by a mixed methanotrophic culture.” Appl. Envir. Microbiology, 57(1), 228–235.
4.
Alvarez‐Cohen, L. M., and McCarty, P. L. (1991c). “Product toxicity and cometabolic competitive inhibition modeling of chloroform and trichloroethylene transformation by methanotrophic resting cells.” Appl. Envir. Microbiology, 57(4), 1031–1037.
5.
Alvarez‐Cohen, L. M., and McCarty, P. L. (1991d). “Two‐stage dispersed‐growth treatment of halogenated aliphatic compounds by cometabolism.” Envir. Sci. Tech., 25(8), 1387–1393.
6.
Arvin, E. (1991). “Biodegradation kinetics of chlorinated aliphatic hydrocarbons with methane oxidizing bacteria in an aerobic fixed biofilm reactor.” Water Res., 25(7), 873–881.
7.
Bilbo, C. M., Arvin, E., Holst, H., and Spliid, H. (1992). “Modelling the growth of methane oxidizing bacteria in a fixed biofilm.” Water Res., 26(3), 301–309.
8.
Broholm, K., Christensen, T. H., and Jensen, B. K. (1992). “Modelling TCE degradation by a mixed culture of methane‐oxidizing bacteria.” Water Res., 26(9), 1177–1185.
9.
Brusseau, G. A., Tsien, H.‐C., Hanson, R. S., and Wackett, L. P. (1991). “Optimization of trichloroethylene oxidation by methanotrophs and the use of a colorimetric assay to detect soluble methane monooxygenase activity.” Biodegradation, 1(1), 19–30.
10.
DiSpirito, A. A., Gulledge, J., Shiemke, A. K., Murrell, J. C., Lidstrom, M. E., and Krema, C. L. (1992). “Trichloroethylene oxidation by the membrane‐associated methane monooxygenase in type I, type II, and type X methanotrophs.” Biodegradation, 2(3), 151–164.
11.
Fan, A. M. (1988). “Trichloroethylene: water contamination and health risk assessment.” Reviews of environmental contamination and toxicology. Springer‐Verlag, New York, N.Y.
12.
Fogel, M. M., Taddeo, A. R., and Fogel, S. (1986). “Biodegradation of chlorinated ethenes by a methane‐utilizing mixed culture.” Appl. Envir. Microbiology, 51(4), 720–724.
13.
Gear, C. W. (1971). Numerical initial value problems in ordinary differential equations. Prentice‐Hall, Englewood Cliffs, N.J.
14.
Henry, S. M., and Grbic‐Galic, D. (1990). “Effect of mineral media on trichloroethylene oxidation by aquifer methanotrophs.” Microbial Ecology, 20, 151–169.
15.
Henry, S. M., and Grbic‐Galic, D. (1991). “Influence of endogenous and exogenous electron donors and trichloroethylene oxidation toxicity on trichloroethylene oxidation by methanotrophic cultures from a groundwater aquifer.” Appl. Envir. Microbiology, 57(1), 236–244.
16.
Kissel, J. C., McCarty, P. L., and Street, R. L. (1984). “Numerical simulation of mixed‐culture biofilm.” J. Envir. Engrg., ASCE, 110(2), 393–411.
17.
Leahy, M. C., Findlay, M., and Fogel, S. (1989). “Biodegradation of chlorinated aliphatics by a methanotrophic consortium in a biological reactor.” Biotreatment: the use of microorganisms in the treatment of hazardous materials and hazardous wastes; Proc., 2nd Nat. Conf., Hazardous Materials Control Research Institute, Silver Spring, Md.
18.
Little, C. D., Palumbo, A. V., Herbes, S. E., Lidstrom, M. E., Tyndall, R. L., and Gilmer, P. J. (1988). “Trichloroethylene biodegradation by a methane‐oxidizing bacterium.” Appl. Envir. Microbiology, 54(4), 951–956.
19.
Matter‐Müller, C., Gujer, W., Giger, W., and Stumm, W. (1980). “Non‐biological elimination mechanisms in a biological sewage treatment plant.” Prog. Water Tech., 12(6), 299–314.
20.
McCarty, P. L. (1975). “Stoichiometry of biological reactions.” Prog. Water Tech., 7(1), 157–172.
21.
McFarland, M. J., Vogel, C. M., and Spain, J. C. (1992). “Methanotrophic cometabolism of trichlorethylene (TCE) in a two stage bioreactor system.” Water Res., 26(2), 259–265.
22.
Namkung, E., and Rittmann, B. E. (1987). “Estimating volatile organic compound emissions from publicly owned treatment works.” J. Water Pollution Control Fed., 59(7), 670–678.
23.
Oldenhuis, R., Oedzes, J. Y., van der Waarde, J. J., and Janssen, D. B. (1991). “Kinetics of chlorinated hydrocarbon degradation by Methylosinus trichosporium OB3b and toxicity of trichloroethylene.” Appl. Envir. Microbiology, 57(1), 7–14.
24.
Oldenhuis, R., Vink, R. L. J. M., Janssen, D. B., and Witholt, B. (1989). “Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase.” Appl. Envir. Microbiology, 55(11), 2819–2826.
25.
Phelps, T. J., Niedzielski, J. J., Schram, R. M., Herbes, S. E., and White, D. C. (1990). “Biodegradation of trichloroethylene in continuous‐recycle expanded‐bed bioreactors.” Appl. Envir. Microbiology, 56(6), 1702–1709.
26.
Rittmann, B. E., and McCarty, P. L. (1981). “Substrate flux into biofilms of any thickness.” J. Envir. Engrg. Div., ASCE, 107(4), 831–849.
27.
Selvakumar, A., and Hsieh, H.‐N. (1988). “Correlation of compound properties with biosorption of organic compounds.” J. Envir. Sci. Health, A23(6), 543–557.
28.
Semprini, L., Hopkins, G. D., Roberts, P. V., Grbic‐Galic, D., and McCarty, P. L. (1991). “A field evaluation of in‐situ biodegradation of chlorinated ethenes. Part 3: Studies of competitive inhibition.” Ground Water, 29(2), 239–250.
29.
Semprini, L., and McCarty, P. L. (1992). “Comparison between model simulations and field results for in‐situ biorestoration of chlorinated aliphatics. Part 2: cometabolic transformations.” Ground Water, 30(1), 37–44.
30.
Semprini, L., Roberts, P. V., Hopkins, G. D., and McCarty, P. L. (1990). “A field evaluation of in‐situ biodegradation of chlorinated ethenes. Part 2: results of biostimulation and biotransformation experiments.” Ground Water, 28(5), 715–727.
31.
Sinclair, C. G., and Topiwala, J. J. (1970). “Model for continuous culture which considers the viability concept.” Biotech. Bioengrg., 12(6), 1069–1079.
32.
Smith, L. H., and McCarty, P. L. (1991). Abstr. Annu. Meet. Am. Soc. Microbiol., Dallas, Texas, Q113.
33.
Speitel, G. E. Jr., and Leonard, J. M. (1992). “A sequencing biofilm reactor for the treatment of chlorinated solvents using methanotrophs.” Water Envir. Res., 64(5), 712–719.
34.
Steen, W. C., and Karickhoff, S. W. (1981). “Biosorption of hydrophobic organic pollutants by mixed microbial populations.” Chemosphere, 10, 27–32.
35.
Strand, S. E., Bjelland, M. D., and Stensel, H. D. (1990). “Kinetics of chlorinated hydrocarbon degradation by suspended cultures of methane‐oxidizing bacteria.” Res. J. Water Pollut. Control Fed., 62(2), 124–129.
36.
Strand, S. E., Seamons, R. M., Bjelland, M. D., and Stensel, H. D. (1988). “Kinetics of methane‐oxidizing biofilms for degradation of toxic organics.” Water Sci. Tech., 20(11/12), 167–173.
37.
Strand, S. E., Wodrich, J. V., and Stensel, H. D. (1991). “Biodegradation of chlorinated solvents in a sparged, methanotrophic biofilm reactor.” Res. J. Water Pollut. Control Fed., 63(6), 859–867.
38.
Strandberg, G. W., Donaldson, T. L., and Farr, L. L. (1989). “Degradation of trichloroethylene and trans‐1,2‐dichloroethylene by a methanotrophic consortium in a fixed‐film, packed‐bed bioreactor.” Envir. Sci. Tech., 23(11), 1422–1425.
39.
Suidan, M. T., and Wang, Y.‐T. (1985). “Unified analysis of biofilm kinetics.” J. Envir. Engrg., ASCE, 111(5), 634–646.
40.
Tsien, H.‐C., Brusseau, G. A., Hanson, R. S., and Wackett, L. P. (1989). “Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b.” Appl. Envir. Microbiology, 55(12), 3155–3161.
41.
Tzesos, M., and Bell, J. P. (1989). “Comparison of the biosorption and desorption of hazardous organic pollutants by live and dead biomass.” Water Res., 23(5), 561–568.
42.
Vannelli, T., Logan, M., Arciero, D. M., and Hooper, A. B. (1990). “Degradation of halogenated aliphatic compounds by the ammonia‐oxidizing bacterium Nitrosomonas europaea.” Appl. Envir. Microbiology, 56(4), 1169–1171.
43.
Wackett, L. P., Brusseau, G. A., Householder, S. R., and Hanson, R. S. (1989). “Survey of microbial oxygenases: trichloroethylene degradation by propane‐oxidizing bacteria.” Appl. Envir. Microbiology, 55(11), 2960–2964.
44.
Wackett, L. P., and Gibson, D. T. (1988). “Degradation of trichloroethylene by toluene dioxygenase in whole‐cell studies with Pseudomonas putida F1.” Appl. Envir. Microbiology, 54(7), 1703–1708.
45.
Wackett, L. P., and Householder, S. R. (1989). “Toxicity of trichloroethylene to Pseudomonas putida F1 is mediated by toluene dioxygenase.” Appl. Envir. Microbiology, 55(10), 2723–2725.
46.
Westrick, J. J., Mello, J. W., and Thomas, R. F. (1984). “The groundwater supply survey.” J. Am. Water Works Assoc., 5(5), 52–59.
47.
Wilke, C. R., and Chang, P. (1955). “Correlation of diffusion coefficients in dilute solutions.” AIChE J., 1(2), 264–270.
48.
Wilkinson, T. G., Topiwala, J. J., and Hamer, G. (1974). “Interactions in a mixed bacterial population growing on methane in continuous culture.” Biotech. Bioengrg., 16(1), 41–59.
49.
Williamson, K., and McCarty, P. L. (1976a). “A model of substrate utilization by bacterial films.” J. Water Pollut. Control Fed., 48(1), 9–24.
50.
Williamson, K., and McCarty, P. L. (1976b). “Verification studies of the biofilm model for bacterial substrate utilization.” J. Water Pollut. Control Fed., 48(2), 281–296.
51.
Wilson, B. H., and White, M. V. (1986). “A fixed‐film bioreactor to treat trichloroethylene‐laden waters from interdiction wells.” Proc., 6th Nat. Symp. and Exposition on Aquifer Restoration and Groundwater Monitoring, National Water Well Assoc., Columbus, Ohio.
52.
Wilson, J. T., and Wilson, B. H. (1985). “Biotransformation of trichloroethylene in soil.” Appl. Envir. Microbiology, 49(1), 242–243.
53.
Winter, R. B., Yen, K.‐M., and Ensley, B. D. (1989). “Efficient degradation of trichloroethylene by a recombinant Escherichia coli.” Bio/Technol., 7(3), 282–285.
Information & Authors
Information
Published In
Journal of Environmental Engineering
Volume 120 • Issue 2 • March 1994
Pages: 379 - 400
Copyright
Copyright © 1994 American Society of Civil Engineers.
History
Received: May 11, 1992
Published online: Mar 1, 1994
Published in print: Mar 1994
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