Vinyl Chloride Biodegradation with Methanotrophic Attached Films
Publication: Journal of Environmental Engineering
Volume 119, Issue 5
Abstract
Methanotrophic degradation of vinyl chloride (VC) is investigated using a laboratory‐scale methanotrophic attached‐film expanded‐bed (MAFEB) bioreactor. This study provides a basis for applying a microbial cometabolizing reaction to practical treatment of toxic chlorinated compounds. The MAFEB reactor was operated at 20°C with influent VC concentrations ranging from 1,800 to 9,600 μg/L and bed hydraulic retention times ranging from 3.7 to 7.6 h. VC effluent concentrations during steady continuous operation ranged from 3 to 140 μg/L, with most values less than 26 μg/L, resulting in removal efficiencies of 96.3% to 99.8%. The maximum continuous‐flow VC degradation rate observed at 20°C was 2.5 mg VC per gram volatile solids (VS) per day [2.5 mg VC/(g VS d)] or 30 mg VC per liter expanded bed per day ), under substrate‐limited conditions. During semibatch runs at 35°C, vinyl chloride degradation rates up to 60 mg VC/ (g VS d) or were observed. Degradation rates increased with temperature between 20°C and 35°C, approximately doubling every 10°C. Dissolved methane concentrations above 0.5 mg/L inhibited VC degradation, with no VC degradation observed with 8 mg/L dissolved methane. The methane consumed during VC degradation was about . Toxic effects were observed after prolonged exposure of the methanotrophic culture to high concentrations of VC.
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References
1.
Alvarez‐Cohen, L., and McCarty, P. L. (1991). “Effects of toxicity, aeration, and reductant supply on trichloroethylene transformation by a mixed methanotrophic culture.” Appl. Envir. Microbiol., 57(1), 228–235.
2.
“Ambient water quality criteria for vinyl chloride.” (1980). EPA/440/5‐80/029, U.S. Environmental Protection Agency, Washington, D.C.
3.
Barrio‐Lage, G. A., Parsons, F. Z., Narbaitz, R. M., Lorenzo, P. A., and Archer, H. E. (1990). “Enhanced anerobic biodegradation of vinyl chloride in ground water.” Environ. Toxicology. and Chem., 9, 403–415.
4.
Brusseau, G. A., Tsien, H‐C., Hanson, R. S., and Wackett, L. P. (1990). “Optimization of trichloroethylene oxidation by methanotrophs and the use of a colorimetric assay to detect soluble methane monooxygenase activity.” Biodegradation, 1, 19–29.
5.
Carter, S. R. (1991). “Low temperature biodegradation of tetrachloroethylene in an anaerobic attached film process,” M.S. thesis, Cornell Univ., Ithaca, N.Y.
6.
Chaudhry, G. R., and Chapalamadugu, S. (1991). “Biodegradation of halogenated organic compounds.” Microbiological Reviews, 55(1), 59–79.
7.
Chu, K‐H. (1991). “Treatment of tetrachloroethylene and trichloroethylene with an anaerobic attached film process,” M.S. thesis, Cornell Univ., Ithaca, N.Y.
8.
Clarkson, W. W. (1986). “Fermentation of particulate organic matter to methane in a thermophillic anaerobic attached film expanded bed reactor,” PhD thesis, Cornell Univ., Ithaca, N.Y.
9.
Craun, G. F. (1984). “Health aspects of ground water pollution.” Ground water pollution microbiology, G. Bitton and C. P. Gerba, eds., John Wiley and Sons, New York, N.Y.
10.
Davis, J. W., and Carpenter, C. L. (1990). “Aerobic biodegradation of vinyl chloride in ground water samples.” Appl. Environ. Microbiol. 56(12), 3878–3880.
11.
Dooley‐Danna, M., Fogel, S., and Findlay, M. (1989). “The sequential anaerobic/aerobic biodegradation of chlorinated ethenes in an aquifer simulator.” Proc., 1989 ASM Meeting. American Society of Microbiology.
12.
Fathepure, B. Z., Nengu, J. P., and Boyd, S. A. (1987). “Anaerobic bacteria that declorinate perchloroethene.” Appl. Environ. Microbiol. 53(11), 2671–2674.
13.
Fennell, D. E., Underhill, S. E., and Jewell, W. J. (1992). “Methanotrophic attached‐film expanded‐bed reactor: system development and biofilm characteristics.” Biotechnol. and Bioengrg., 40(11), 1218–1232.
14.
Fogel, M. M., Taddeo, A. R., and Fogel, S. (1986). “Biodegradation of chlorinated ethenes by a methane‐utilizing mixed culture.” J. Appl. Environ. Microbiol., 51(4), 720–724.
15.
Fogel, S. (1990). “Sequential anaerobic/aerobic biodegradation of chlorinated ethenes.” Proc., 199th ACS Meeting, Div. of Environ. Chem., American Chemical Society, Washington, D.C.
16.
Fogel, S., Findlay, M., Moore, A., and Leahy, M. (1987). “Biodegradation of chlorinated chemicals in ground water by methane oxidizing bacteria.” Nat. Water Well Assn. Conf. on Petroleum Hydrocarbons and Organic Chemicals in Ground Water: Prevention, Detection and Restoration.
17.
Freedman, D. L., and Gossett, J. M. (1989). “Biological reductive dechlorination of tetrachloroethylene and trichloroethylene to ethylene under methanogenic conditions.” Appl. Environ. Microbiol., 55(9), 2144–2151.
18.
Gossett, J. M. (1987). “Measurement of Henry's law constants for C1 and C2 chlorinated hydrocarbons.” Envir. Sci. and Tech., 21, 202–208.
19.
Hartmans, S., de Bont, J. A. M., Tramper, J., and Luyben, K. Ch. A. M. (1985). “Bacterial degradation of vinyl chloride.” Biotech. Letters, 7(6), 383–388.
20.
Henry, S. M., and Grbic‐Galic, D. (1991). “Inhibition of trichloroethylene oxidation by the transformation intermediate carbon monoxide.” Appl. Environ. Microbiol., 57(6), 1770–1776.
21.
Infante, P. F., and Tsongas, T. A. (1982). “Mutagenic and oncogenic effects of chloromethanes, chloroethanes and halogenated analogues of vinyl chloride.” Envir. Sci. Res., 25, 301–327.
22.
Jewell, W. J., Fennell, D. E., Nelson, Y. M., Underhill, S. E., White, T. E., and Wilson, M. S. (1990). “Methanotrophs and methanogens for pollution control: PCE and TCE removal from ground water and macro nutrient removals from wastewater.” Res. Rep., Gas Research Institute and Radian Corp., Chicago, Ill.
23.
Leak, D. J., and Dalton, H. (1986). “Growth yields of methanotrophs: 1. effect of copper on the energetics of methane oxidation.” Appl. Microbiol. Biotechnol., 23, 470–476.
24.
Lee, C. C., Bhandari, J. C., Winston, J. M., House, W. B., Dixon, R. L., and Woods, J. S. (1978). “Carcinogenicity of vinyl chloride and vinylidene chloride.” J. Toxic. Envir. Health, 4, 15–30.
25.
Love, O. T., and Eilers, R. G. (1982). “Treatment of drinking water containing trichloroethylene and related industrial solvents.” J. Amer. Water Works Assn., 74, 413–425.
26.
Maltoni, C. (1977). “Vinyl chloride carcinogenicity: an experimental model for carcinogenicity studies.” Origins of human cancer. H. H. Hiatt, J. D. Watson, and J. A. Winsten, eds., Cold Spring Harbor, N.Y., 119–146.
27.
Milde, G., Nerger, M., and Mergler, R. (1988). “Biological degradation of volatile chlorinated hydrocarbons in ground water.” Water Sci. Technol., 20(3), 67–73.
28.
Molton, P. M., Hallen, R. T., and Pyne, J. W. (1987). “Study of vinyl chloride formation at landfill sites in California.” BNWL‐2311206978, Batelle Pacific North‐west Laboratories, Richland, Wash.
29.
Oldenhuis, R., Oedzes, J. Y., van der Waarde, J. J., and Janssen, D. B. (1991). “Kinetics of chlorinated hydrocarbon degradation by methylosinus trichosporium OB3b and toxicity of trichloroethylene.” Appl. Environ. Microbiol., 57(1), 7–14.
30.
Parsons, F., Barrio‐Lage, G., and Rice, R. (1985). “Biotransformation of chlorinated organic solvents in static microcosms.” Environ. Toxic. Chem., 4, 739–742.
31.
Parsons, F., Wood, P. R., and DeMarco, J. (1984). “Transformations of tetra‐chloroethylene and trichloroethylene in microcosms and ground water.” J. Amer. Waterworks Assn., 76, 56–59.
32.
Roberts, P. V., Simprini, L., Hopkins, G. D., Grbic‐Galic, D., McCarty, P. L., and Reinhard, M. (1989). “In‐situ aquifer restoration of chlorinated aliphatics by methanotrophic bacteria.” U.S. EPA Rep. #600/2‐89/033, U.S. Environmental Protection Agency, Washington, D.C.
33.
Standard methods for the examination of water and wastewater. (1985). 16th Ed., Am. Pub. Health Assn., Am. Water Works Assn., Water Poll. Contr. Fed., Washington, D.C.
34.
Tsien, H.‐C., Brusseau, G. A., Hanson, R. S., and Wacket, L. P. (1989). “Biodegradation of trichloroethylene by methylosinus trichosporium OB3b.” Appl. Environ. Microbiol., 55(12), 3155–3161.
35.
Vary, P. S., and Johnson, M. J. (1967). “Cell yield of bacteria grown on methane.” Appl. Microbiol., 15(6), 1473–1478.
36.
Vogel, T. M., and McCarty, P. L. (1985). “Biotransformation of tetrachloroethylene to trichloroethylene, dichloroethylene, vinyl chloride and carbon dioxide under methanogenic conditions.” Appl. Environ. Microbiol., 49(5), 1080–1083.
37.
Vogel, T. M., Criddle, C. S., and McCarty, P. L. (1987). “Transformations of halogenated aliphatic compounds.” Environ. Sci. Technol., 21(8), 722–736.
38.
Westrick, J. J., Mello, J. W., and Thomas, R. F. (1984). “The ground water supply survey.” J. Amer. Water Works Assoc., 76, 52–59.
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Copyright © 1993 American Society of Civil Engineers.
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Received: Feb 24, 1992
Published online: Sep 1, 1993
Published in print: Sep 1993
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