TECHNICAL PAPERS
Oct 1, 1990

Comparison of Serum Bottle Toxicity Test with OECD Method

Publication: Journal of Environmental Engineering
Volume 116, Issue 6

Abstract

Serum bottle (SB) activated sludge toxicity test procedure is compared with the OECD procedure. The purpose of this study is to compare and contrast the two testing procedures so that one can complement the other. If the toxicity measurements obtained from the two testing procedures are comparable, users can select either procedure based on time constraint and equipment availability. Seventeen organic toxicants are selected for the comparison. The results show: (1) For chemicals with low volatility, the OECD procedure and SB procedure give comparable results for obtaining the IC50 of organic toxicants; and (2) for volatile chemicals, the SB procedure can be reliably used whereas the OECD procedure cannot because aeration in an open vessel strips out the chemical of interest and the culture is subjected to a concentration decrease with time. Four phenolic compounds are used for studying the effect on the IC50 results due to the origin of the activated sludge. Lab‐activated sludge grown on a complex, defined organic medium and sewage activated sludge gives comparable IC50 results.

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References

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Blum, D. J. W. (1989). “Chemical toxicity to environmental bacteria: Quantitative structure activity relationships and interspecies correlations and comparisons,” thesis presented to Drexel University, at Philadelphia, Pa., in partial fulfillment of the requirement for the degree of Doctor of Philosophy.
2.
Hine, J., and Mookerjee, P. K. (1975). “The intrinsic hydrophilic character of organic compounds: Correlations in terms of structural contributions.” J. Organic Chemistry, 40(3), 292–298.
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Nirmalakhandan, N. (1988). “Prediction of aqueous solubility and Henry's constant from molecular structure,” thesis presented to Drexel University, at Philadelphia, Pa., in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
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OECD guideline for testing of chemicals: Activated sludge, respiration inhibition test. (1984). Organization for Economic Cooperation and Development, Paris, France.
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OECD. (1987). “The use of biological tests for water pollution assessment and control.” Environment Monographs No. 11, Organization for Economic Cooperation and Development, Paris, France.
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Owen, W. F., et al. (1979). “Bioassay for monitoring biochemical methane potential and anaerobic toxicity.” Water Res., 13(6), 485–492.

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Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 116Issue 6October 1990
Pages: 1076 - 1084

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Published online: Oct 1, 1990
Published in print: Oct 1990

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Authors

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Ning H. Tang
Prof., Dept. of Civ. Engrg., Univ. of Puerto Rico, Mayaguez, PR 00708
Diane J. W. Blum
Private Consultant, 112 Clwyd Rd., Bala Cynwyd, PA 19004
Richard E. Speece
Centennial Prof., Civ. and Envir. Engrg. Dept., Vanderbilt Univ., Nashville, TN 37235

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