Abstract
Dairy wastewater (DWW) is nutritionally rich and extremely hazardous to the environment if discharged untreated. The conventional treatment is time-, labor-, and energy-intensive. A tailor-made microbial consortium converted DWW into a biofertilizer with the ability to enhance biomass and yield in mung bean (Vigna radiata var. MEHA). The consortium produced ammonia from DWW at a rate of within 16 h of incubation in a biofilm bioreactor at 37°C with highest production of demonstrating 41.83% nitrate and 45.83% phosphate removal. The scalability was tested at ambient temperature in a 72-L bioreactor with an ammonia production rate of . Irrigation using the treated effluent resulted in a 1.7-fold increase in biomass, 49-fold decrease in root nodulation, and 2.6-fold increase in seed yield in mung bean while providing protection from aphid (Aphis craccivora Koch) infestation. The total wastewater was converted into biofertilizer for use as a substitute for chemical fertilizer and fresh water for irrigation. This approach makes DWW management not only a zero-discharge process but also a self-sustainable one.
Get full access to this article
View all available purchase options and get full access to this article.
Acknowledgments
We acknowledge the financial assistance from the Ministry of Human Resource Development, Government of India (GOI) under the Frontier Area of Science and Technology Scheme; Department of Biotechnology, GOI under the Twinning project; and the University Grant Commission–Department of Atomic Energy for student fellowship. We acknowledge Tripura University for providing the laboratory infrastructure and field trial facility for conducting the experiments, and Maulana Abul Kalam Azad University of Technology, West Bengal for the bioreactor setup. We thank Mr. Nandan Prabhuna, Professor Amitava Mukherjee, and Professor Ashoke Ranjan Thakur for the scientific discussion during the course of the work.
References
Asri, M., S. Elabed, S. I. Koraichi, and N. E. Ghachtouli. 2018. “Biofilm-based systems for industrial wastewater treatment.” In Handbook of environmental materials management, edited by C. Hussain, 1–21. Cham, Switzerland: Springer.
Azizi, S., A. Valipour, and T. Sithebe. 2013. “Evaluation of different wastewater treatment processes and development of a modified attached growth bioreactor as a decentralized approach for small communities.” Sci. World J. 2013: 1–8. https://doi.org/10.1155/2013/156870.
Belancon, D., M. C. Fuzzato, D. R. S. Gomes, G. C. V. Cichello, S. C. D. Pinho, R. Ribeiro, and G. Tommaso. 2010. “A comparison of two bench-scale anaerobic systems used for the treatment of dairy effluents.” Int. J. Dairy Technol. 63 (2): 290–296. https://doi.org/10.1111/j.1471-0307.2010.00569.x.
Biswas, T., D. Chatterjee, S. Barman, A. Chakraborty, N. Halder, S. Banerjee, and S. Ray Chaudhuri. 2019. “Cultivable bacterial community analysis of dairy activated sludge for value addition to dairy wastewater.” Microbiol. Biotechnol. Lett.
Britz, T. J., C. V. Schalkwyk, and Y. T. Hung. 2006. “Treatment of dairy processing wastewaters.” In Waste treatment in the food processing industry, 1–28. Boca Raton, FL: Taylor & Francis.
Budavari, S. 1996. The Merck index: An encyclopedia of chemicals, drugs, and biological. 12th ed. Whitehouse Station, NJ: Merck.
Busaidi, A. A., and M. Ahmed. 2017. “Maximum use of treated wastewater in agriculture.” In Water resources in arid areas: The way forward, 371–382. Cham, Switzerland: Springer.
Cataldo, D. A., M. Maroon, L. E. Schrader, and V. L. Youngs. 1975. “Rapid colorimetric determination of nitrate in plant tissues by nitration of salicylic acid.” Commun. Soil Sci. Plant Anal. 6 (1): 71–80. https://doi.org/10.1080/00103627509366547.
Dague, R. R., C. E. Habben, and S. R. Pidaparti. 1992. “Initial studies on the anaerobic sequencing batch reactor.” Water Sci. Technol. 26 (9–11): 2429–2432. https://doi.org/10.2166/wst.1992.0754.
Das, S., I. Mukherjee, M. Sudarshan, T. P. Sinha, A. R. Thakur, and S. Ray Chaudhuri. 2012. “Bacterial isolates of marine coast as commercial producers of protease.” Online J. Biol. Sci. 12 (3): 96–107. https://doi.org/10.3844/ojbsci.2012.96.107.
Deepak, M., J. S. Sudarasan, V. T. Deeptha, and G. Baskar. 2012. “Low cost DWW treatment using constructed wetland.” J. Inst. Public Health Eng. 3: 55–60.
Deniges, G. 1920. “Reaction de coloration extremement sensible des phosphates et des arseniates. Ses applications.” C. R. Acad. Sci. Paris 171: 802–804.
Gautam, S. P. 2015. “Guide manual: Water and wastewater analysis. Central Pollution Control Board, Ministry of Environment and Forests, Government of India.” Accessed May 13, 2019. http://cpcb.nic.in/openpdffile.php?id=UmVwb3J0RmlsZXMvMjA0XzE1MjQ2NTA4OTNfbWVkaWFwaG90bzEyODI3LnBkZg.
Ghoshal, T., S. Ghosh, A. Saha, N. Haldar, A. R. Thakur, and S. Ray Chaudhuri. 2014. “Combination of conventional and in-silico approach for identifying an industrially important isolate of Aeromonas.” Online J. Biol. Sci. 14 (2): 70–83. https://doi.org/10.3844/ojbsci.2014.70.83.
Hao, R., S. Li, J. Li, and C. Meng. 2013. “Denitrification of simulated municipal wastewater treatment plant effluent using a three-dimensional biofilm-electrode reactor: Operating performance and bacterial community.” Bioresour. Technol. 143 (Sep): 178–186. https://doi.org/10.1016/j.biortech.2013.06.001.
Hawke, R. M., and S. A. Summers. 2006. “Effects of land application of farm dairy effluent on soil properties: A literature review.” N. Z. J. Agric. Res. 49 (3): 307–320. https://doi.org/10.1080/00288233.2006.9513721.
Jiao, Y., G. D. Cody, A. K. Harding, P. Wilmes, M. Schrenk, K. E. Wheeler, J. F. Banfield, and M. P. Thelen. 2010. “Characterization of extracellular polymeric substances from acidophilic microbial biofilms.” Appl. Environ. Microbiol. 76 (9): 2916–2922. https://doi.org/10.1128/AEM.02289-09.
Karadag, D., O. E. Koroglu, B. Ozkaya, M. Cakmakci, S. Heaven, C. Banks, and A. Serna-Maza. 2015. “Anaerobic granular reactors for the treatment of DWW: A review.” Int. J. Dairy Technol. 68 (4): 459–470. https://doi.org/10.1111/1471-0307.12252.
Kushwaha, J. P., V. C. Srivastava, and I. D. Mall. 2011. “An overview of various technologies for the treatment of dairy wastewaters.” Crit. Rev. Food Sci. Nutr. 51 (5): 442–452. https://doi.org/10.1080/10408391003663879.
Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. “Protein measurement with the Folin phenol reagent.” J. Biol. Chem. 193 (1): 265–275.
Marnellos, G., and M. Stoukides. 1998. “Ammonia synthesis at atmospheric pressure.” Science 282 (5386): 98–100. https://doi.org/10.1126/science.282.5386.98.
Martin, R., N. Soberon, M. Vaneechoutte, A. B. Florez, F. Vazquez, and J. E. Suarez. 2008. “Characterization of indigenous vaginal lactobacilli from healthy women as probiotic candidates.” Int. Microbiol. 11 (4): 261–266. https://doi.org/10.2436/20.1501.01.70.
Ministry of Environment, Forest and Climate Change, Government of India. 2016. “Re-Categorisation of Industries a landmark decision, new category of white industries will not require environmental clearance.” Accessed May 13, 2019. http://pib.nic.in/newsite/PrintRelease.aspx?relid=137373.
Mukherjee, I., A. Giri, and P. Barat. 2016a. “Quantitative characterization of sulphate reduction data obtained from a biofilm based bioreactor—Part II.” In Life science: Recent innovations and research, 103–120. New Delhi, India: International Research Publication House.
Mukherjee, I., A. Giri, C. Sen, R. Sebait, and P. Barat. 2016b. “Quantitative characterization of sulphate reduction data obtained from a biofilm based bioreactor—Part I.” In Life science: Recent innovations and research, 81–102. New Delhi, India: International Research Publication House.
National Dairy Development Board. 2019. “Milk production across countries.” Accessed May 13, 2019. https://www.nddb.coop/information/stats/across.
O’Neil, M. J. 2013. The Merck index: An encyclopedia of chemicals, drugs, and biologicals, 88. Cambridge, UK: Royal Society Chemistry.
Parkin, M. F., and K. R. Marshall. 1976. “Spray irrigation disposal of dairy factory effluent: A review of current practice in New Zealand.” N. Z. J. Dairy Sci. 11: 196–205.
Prazeres, A. R., F. Carvalho, J. Rivas, M. Patanita, and J. Dores. 2013. “Pretreated cheese whey wastewater management by agricultural reuse: Chemical characterization and response of tomato plants Lycopersicon esculentum Mill. under salinity conditions.” Sci. Total Environ. 463–464 (Oct): 943–951. https://doi.org/10.1016/j.scitotenv.2013.06.080.
Qasim, W., and A. V. Mane. 2013. “Characterization and treatment of selected food industrial effluents by coagulation and adsorption techniques.” Water Resour. Ind. 4 (Dec): 1–12. https://doi.org/10.1016/j.wri.2013.09.005.
Ramasamy, E. V., S. Gajalakshmi, R. Sanjeevi, M. N. Jithesh, and S. A. Abbasi. 2004. “Feasibility studies on the treatment of dairy wastewaters with upflow anaerobic sludge blanket reactors.” Bioresour. Technol. 93 (2): 209–212. https://doi.org/10.1016/j.biortech.2003.11.001.
Ray Chaudhuri, S., et al. 2016a. “Developing tailor made microbial consortium for effluent remediation.” In Nuclear material performance, edited by R. O. A. Rahman and H. E. D. M. Saleh, 17–35. Rijeka, Croatia: Intech.
Ray Chaudhuri, S., et al. 2016b. “Novel microbial system developed from low level radioactive waste treatment plant for environmental sustenance.” In Management of hazardous wastes, edited by H. E. D. M. Saleh and R. O. A. Rahman, 121–154. Rijeka, Croatia: Intech.
Ray Chaudhuri, S., et al. 2017. “Microbe-based strategy for plant nutrient management.” In Wastewater treatment and resource recovery, edited by R. Farooq and Z. Ahmed, 38–55. Rijeka, Croatia: Intech.
Ray Chaudhuri, S. 2019. “Scientific intervention for waste water treatment: Recycle and reuse.” In Conf. Proc. (Lead Papers) of River and Water Management for Sustainable Development with Policy Perspectives, 25–29. Tripura, India: Tripura Univ.
Ray Chaudhuri, S., I. Mukherjee, and A. R. Thakur. 2013. Microbial consortium for nitrate and phosphate sequestration for environmental sustenance. Indian Application No. 1179/KOL/2013.
Ray Chaudhuri, S., I. Mukherjee, and A. R. Thakur. 2014. Microbial consortium for nitrate and phosphate sequestration for environmental sustenance. International application published under The Patent Cooperation Treaty (PCT) No. WO 2015/056185 Al.
Rittmann, B. E., and P. E. McCarty. 2012. “Biofilm kinetics.” In Environmental biotechnology: Principles and applications, edited by G. Tchobanoglous, 207–260. New Delhi, India: Tata McGraw Hill Education.
Saha, A., et al. 2018. “Simultaneous sequestration of nitrate and phosphate from wastewater using a tailor made bacterial consortium in biofilm bioreactor.” J. Chem. Technol. Biotechnol. 93: (5): 1279–1289. https://doi.org/10.1002/jctb.5487.
Shete, B. S., and N. P. Shinkar. 2013. “Dairy industry wastewater sources, characteristics and its effects on environment.” Int. J. Curr. Eng. Technol. 3 (5): 1611–1615.
UNDESA (United Nations Department of Economic and Social Affairs). 2014. “International decade for action ‘Water of life 2005–2015’.” Accessed August 4, 2019. https://www.un.org/waterforlifedecade/scarcity.shtml.
Vourch, M., B. Balannec, B. Chaufer, and G. Dorange. 2005. “Nanofiltration and reverse osmosis of model process waters from the dairy industry to produce water for reuse.” Desalination 172 (3): 245–256. https://doi.org/10.1016/j.desal.2004.07.038.
Information & Authors
Information
Published In
Copyright
©2019 American Society of Civil Engineers.
History
Received: May 31, 2019
Accepted: Aug 26, 2019
Published online: Oct 22, 2019
Published in print: Jan 1, 2020
Discussion open until: Mar 22, 2020
Authors
Metrics & Citations
Metrics
Citations
Download citation
If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.